PMID- 24842370
OWN - NLM
STAT- MEDLINE
DCOM- 20140820
LR  - 20221109
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 211
IP  - 6
DP  - 2014 Jun 2
TI  - Lymph node stromal cells acquire peptide-MHCII complexes from dendritic cells and 
      induce antigen-specific CD4(+) T cell tolerance.
PG  - 1153-66
LID - 10.1084/jem.20132000 [doi]
AB  - Dendritic cells (DCs), and more recently lymph node stromal cells (LNSCs), have 
      been described to tolerize self-reactive CD8(+) T cells in LNs. Although LNSCs 
      express MHCII, it is unknown whether they can also impact CD4(+) T cell 
      functions. We show that the promoter IV (pIV) of class II transactivator (CIITA), 
      the master regulator of MHCII expression, controls endogenous MHCII expression by 
      LNSCs. Unexpectedly, LNSCs also acquire peptide-MHCII complexes from DCs and 
      induce CD4(+) T cell dysfunction by presenting transferred complexes to naive 
      CD4(+) T cells and preventing their proliferation and survival. Our data reveals 
      a novel, alternative mechanism where LN-resident stromal cells tolerize CD4(+) T 
      cells through the presentation of self-antigens via transferred peptide-MHCII 
      complexes of DC origin.
CI  - (c) 2014 Dubrot et al.
FAU - Dubrot, Juan
AU  - Dubrot J
AD  - Department of Pathology and Immunology, University of Geneva Medical School, 1211 
      Geneva, Switzerland.
FAU - Duraes, Fernanda V
AU  - Duraes FV
AD  - Department of Pathology and Immunology, University of Geneva Medical School, 1211 
      Geneva, Switzerland.
FAU - Potin, Lambert
AU  - Potin L
AD  - Laboratory of Lymphatic and Cancer Bioengineering, Institute of Bioengineering 
      and Swiss Institute for Experimental Cancer Research (ISREC), School of Life 
      Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 Lausanne, 
      Switzerland.
FAU - Capotosti, Francesca
AU  - Capotosti F
AD  - Laboratory of Lymphatic and Cancer Bioengineering, Institute of Bioengineering 
      and Swiss Institute for Experimental Cancer Research (ISREC), School of Life 
      Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 Lausanne, 
      Switzerland.
FAU - Brighouse, Dale
AU  - Brighouse D
AD  - Department of Pathology and Immunology, University of Geneva Medical School, 1211 
      Geneva, Switzerland.
FAU - Suter, Tobias
AU  - Suter T
AD  - Department of Neurology, Section of Neuroimmunology and MS Research, University 
      Hospital Zurich, 8091 Zurich, Switzerland.
FAU - LeibundGut-Landmann, Salome
AU  - LeibundGut-Landmann S
AD  - Institute of Microbiology, Swiss Federal Institute of Technology Zurich, 8093 
      Zurich, Switzerland.
FAU - Garbi, Natalio
AU  - Garbi N
AD  - Institute of Molecular Medicine and Institute of Experimental Immunology, 
      University of Bonn, 53105 Bonn, Germany Institute of Molecular Medicine and 
      Institute of Experimental Immunology, University of Bonn, 53105 Bonn, Germany.
FAU - Reith, Walter
AU  - Reith W
AD  - Department of Pathology and Immunology, University of Geneva Medical School, 1211 
      Geneva, Switzerland.
FAU - Swartz, Melody A
AU  - Swartz MA
AD  - Laboratory of Lymphatic and Cancer Bioengineering, Institute of Bioengineering 
      and Swiss Institute for Experimental Cancer Research (ISREC), School of Life 
      Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 Lausanne, 
      Switzerland ISREC, SV, EPFL, 1015 Lausanne, Switzerland Stephanie.hugues@unige.ch 
      melody.swartz@epfl.ch.
FAU - Hugues, Stephanie
AU  - Hugues S
AD  - Department of Pathology and Immunology, University of Geneva Medical School, 1211 
      Geneva, Switzerland Stephanie.hugues@unige.ch melody.swartz@epfl.ch.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140519
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (MHC class II transactivator protein)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Peptides)
RN  - 0 (Trans-Activators)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Antigens/*immunology
MH  - CD4-Positive T-Lymphocytes/*immunology/metabolism
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/metabolism
MH  - Flow Cytometry
MH  - Gene Expression/drug effects/immunology
MH  - Histocompatibility Antigens Class II/genetics/immunology/metabolism
MH  - Immune Tolerance/*immunology
MH  - Interferon-gamma/immunology/pharmacology
MH  - Lymph Nodes/cytology/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Nuclear Proteins/genetics/immunology/metabolism
MH  - Peptides/immunology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stromal Cells/*immunology/metabolism
MH  - Trans-Activators/genetics/immunology/metabolism
PMC - PMC4042642
EDAT- 2014/05/21 06:00
MHDA- 2014/08/21 06:00
PMCR- 2014/12/02
CRDT- 2014/05/21 06:00
PHST- 2014/05/21 06:00 [entrez]
PHST- 2014/05/21 06:00 [pubmed]
PHST- 2014/08/21 06:00 [medline]
PHST- 2014/12/02 00:00 [pmc-release]
AID - jem.20132000 [pii]
AID - 20132000 [pii]
AID - 10.1084/jem.20132000 [doi]
PST - ppublish
SO  - J Exp Med. 2014 Jun 2;211(6):1153-66. doi: 10.1084/jem.20132000. Epub 2014 May 
      19.