PMID- 24844151
OWN - NLM
STAT- MEDLINE
DCOM- 20150723
LR  - 20181202
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 32
IP  - 50
DP  - 2014 Nov 28
TI  - Proteome-wide screening of the European porcine reproductive and respiratory 
      syndrome virus reveals a broad range of T cell antigen reactivity.
PG  - 6828-37
LID - S0264-410X(14)00592-1 [pii]
LID - 10.1016/j.vaccine.2014.04.054 [doi]
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) is a rapidly 
      evolving and diversifying pathogen necessitating the development of improved 
      vaccines. Immunity to PRRSV is not well understood although there are data 
      suggesting that virus-specific T cell IFN-gamma responses play an important role. We 
      therefore aimed to better characterise the T cell response to genotype 1 
      (European) PRRSV by utilising a synthetic peptide library spanning the entire 
      proteome and a small cohort of pigs rendered immune to PRRSV-1 Olot/91 by 
      repeated experimental infection. Using an IFN-gamma ELISpot assay as a read-out, we 
      were able to identify 9 antigenic regions on 5 of the viral proteins and 
      determine the corresponding responder T cell phenotype. The diversity of the 
      IFN-gamma response to PRRSV proteins suggests that antigenic regions are scattered 
      throughout the proteome and no one single antigen dominates the T cell response. 
      To address the identification of well-conserved T cell antigens, we subsequently 
      screened groups of pigs infected with a closely related avirulent PRRSV-1 strain 
      (Lelystad) and a divergent virulent subtype 3 strain (SU1-Bel). Whilst T cell 
      responses from both groups were observed against many of the antigens identified 
      in the first study, animals infected with the SU1-Bel strain showed the greatest 
      response against peptides representing the non-structural protein 5. The 
      proteome-wide peptide library screening method used here, as well as the antigens 
      identified, warrant further evaluation in the context of next generation vaccine 
      development.
CI  - Crown Copyright (c) 2014. Published by Elsevier Ltd. All rights reserved.
FAU - Mokhtar, Helen
AU  - Mokhtar H
AD  - Virology Department, Animal Health and Veterinary Laboratories Agency (AHVLA), 
      Addlestone KT15 3NB, UK; Department of Microbial & Cellular Sciences, University 
      of Surrey, Guildford GU2 7XH, UK.
FAU - Eck, Melanie
AU  - Eck M
AD  - Institute of Virology and Immunology (IVI), Sensemattstrasse 293, CH-3147 
      Mittelhausern, Switzerland; Graduate School for Cellular and Biomedical Sciences, 
      University of Bern, Switzerland.
FAU - Morgan, Sophie B
AU  - Morgan SB
AD  - Virology Department, Animal Health and Veterinary Laboratories Agency (AHVLA), 
      Addlestone KT15 3NB, UK.
FAU - Essler, Sabine E
AU  - Essler SE
AD  - Department of Pathobiology, Institute of Immunology, University of Veterinary 
      Medicine Vienna, Veterinaerplatz 1, Vienna A-1210, Austria.
FAU - Frossard, Jean-Pierre
AU  - Frossard JP
AD  - Virology Department, Animal Health and Veterinary Laboratories Agency (AHVLA), 
      Addlestone KT15 3NB, UK.
FAU - Ruggli, Nicolas
AU  - Ruggli N
AD  - Institute of Virology and Immunology (IVI), Sensemattstrasse 293, CH-3147 
      Mittelhausern, Switzerland.
FAU - Graham, Simon P
AU  - Graham SP
AD  - Virology Department, Animal Health and Veterinary Laboratories Agency (AHVLA), 
      Addlestone KT15 3NB, UK. Electronic address: simon.graham@ahvla.gsi.gov.uk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140517
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antigens, Viral)
RN  - 0 (Proteome)
RN  - 0 (Viral Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/*immunology
MH  - Enzyme-Linked Immunospot Assay
MH  - Interferon-gamma/metabolism
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Proteome/*immunology
MH  - Swine
MH  - T-Lymphocytes/*immunology
MH  - Viral Proteins/*immunology
OTO - NOTNLM
OT  - Antigen identification
OT  - ELISpot assay
OT  - IFN-gamma
OT  - Porcine
OT  - Porcine reproductive and respiratory syndrome virus
OT  - T cell
EDAT- 2014/05/23 06:00
MHDA- 2015/07/24 06:00
CRDT- 2014/05/22 06:00
PHST- 2013/12/19 00:00 [received]
PHST- 2014/04/11 00:00 [revised]
PHST- 2014/04/17 00:00 [accepted]
PHST- 2014/05/22 06:00 [entrez]
PHST- 2014/05/23 06:00 [pubmed]
PHST- 2015/07/24 06:00 [medline]
AID - S0264-410X(14)00592-1 [pii]
AID - 10.1016/j.vaccine.2014.04.054 [doi]
PST - ppublish
SO  - Vaccine. 2014 Nov 28;32(50):6828-37. doi: 10.1016/j.vaccine.2014.04.054. Epub 
      2014 May 17.