PMID- 24844661
OWN - NLM
STAT- MEDLINE
DCOM- 20150713
LR  - 20231213
IS  - 1364-3703 (Electronic)
IS  - 1464-6722 (Print)
IS  - 1364-3703 (Linking)
VI  - 15
IP  - 9
DP  - 2014 Dec
TI  - Manipulation of two alpha-endo-beta-1,4-glucanase genes, AtCel6 and GmCel7, reduces 
      susceptibility to Heterodera glycines in soybean roots.
PG  - 927-39
LID - 10.1111/mpp.12157 [doi]
AB  - Plant endo-beta-1,4-glucanases (EGases) include cell wall-modifying enzymes that are 
      involved in nematode-induced growth of syncytia (feeding structures) in 
      nematode-infected roots. EGases in the alpha- and beta-subfamilies contain signal 
      peptides and are secreted, whereas those in the gamma-subfamily have a 
      membrane-anchoring domain and are not secreted. The Arabidopsis alpha-EGase 
      At1g48930, designated as AtCel6, is known to be down-regulated by beet cyst 
      nematode (Heterodera schachtii) in Arabidopsis roots, whereas another alpha-EGase, 
      AtCel2, is up-regulated. Here, we report that the ectopic expression of AtCel6 in 
      soybean roots reduces susceptibility to both soybean cyst nematode (SCN; 
      Heterodera glycines) and root knot nematode (Meloidogyne incognita). Suppression 
      of GmCel7, the soybean homologue of AtCel2, in soybean roots also reduces the 
      susceptibility to SCN. In contrast, in studies on two gamma-EGases, both ectopic 
      expression of AtKOR2 in soybean roots and suppression of the soybean homologue of 
      AtKOR3 had no significant effect on SCN parasitism. Our results suggest that 
      secreted alpha-EGases are likely to be more useful than membrane-bound gamma-EGases in 
      the development of an SCN-resistant soybean through gene manipulation. 
      Furthermore, this study provides evidence that Arabidopsis shares molecular 
      events of cyst nematode parasitism with soybean, and confirms the suitability of 
      the Arabidopsis-H. schachtii interaction as a model for the soybean-H. glycines 
      pathosystem.
CI  - (c) 2014 BSPP AND JOHN WILEY & SONS LTD.
FAU - Woo, Mi-Ok
AU  - Woo MO
AD  - Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, 20705, 
      USA.
FAU - Beard, Hunter
AU  - Beard H
FAU - MacDonald, Margaret H
AU  - MacDonald MH
FAU - Brewer, Eric P
AU  - Brewer EP
FAU - Youssef, Reham M
AU  - Youssef RM
FAU - Kim, Hyunsoon
AU  - Kim H
FAU - Matthews, Benjamin F
AU  - Matthews BF
LA  - eng
SI  - GENBANK/AW310136
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140707
PL  - England
TA  - Mol Plant Pathol
JT  - Molecular plant pathology
JID - 100954969
RN  - 9004-34-6 (Cellulose)
SB  - IM
MH  - Animals
MH  - Cellulose/*genetics
MH  - Disease Resistance
MH  - Disease Susceptibility
MH  - Female
MH  - Gene Expression Regulation, Plant
MH  - *Genes, Plant
MH  - Molecular Sequence Data
MH  - Plant Diseases/immunology/*parasitology
MH  - Plant Roots/genetics/*parasitology
MH  - Plants, Genetically Modified
MH  - Polymerase Chain Reaction
MH  - Glycine max/*enzymology/genetics/immunology/*parasitology
MH  - Tylenchoidea/*physiology
PMC - PMC6638630
OTO - NOTNLM
OT  - Arabidopsis
OT  - EGase
OT  - Heterodera glycines
OT  - Meloidogyne incognita
OT  - soybean
EDAT- 2014/05/23 06:00
MHDA- 2015/07/15 06:00
PMCR- 2014/07/07
CRDT- 2014/05/22 06:00
PHST- 2014/05/22 06:00 [entrez]
PHST- 2014/05/23 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
PHST- 2014/07/07 00:00 [pmc-release]
AID - MPP12157 [pii]
AID - 10.1111/mpp.12157 [doi]
PST - ppublish
SO  - Mol Plant Pathol. 2014 Dec;15(9):927-39. doi: 10.1111/mpp.12157. Epub 2014 Jul 7.