PMID- 24845419
OWN - NLM
STAT- MEDLINE
DCOM- 20150219
LR  - 20211021
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 392
IP  - 1-2
DP  - 2014 Jul 5
TI  - Dehydroepiandrosterone-induces miR-21 transcription in HepG2 cells through 
      estrogen receptor beta and androgen receptor.
PG  - 23-36
LID - S0303-7207(14)00149-X [pii]
LID - 10.1016/j.mce.2014.05.007 [doi]
AB  - Although oncomiR miR-21 is highly expressed in liver and overexpressed in 
      hepatocellular carcinoma (HCC), its regulation is uncharacterized. We examined 
      the effect of physiologically relevant nanomolar concentrations of 
      dehydroepiandrosterone (DHEA) and DHEA sulfate (DHEA-S) on miR-21 expression in 
      HepG2 human hepatoma cells. 10nM DHEA and DHEA-S increase pri-miR-21 
      transcription in HepG2 cells. Dietary DHEA increased miR-21 in vivo in mouse 
      liver. siRNA and inhibitor studies suggest that DHEA-S requires desulfation for 
      activity and that DHEA-induced pri-miR-21 transcription involves metabolism to 
      androgen and estrogen receptor (AR and ER) ligands. Activation of ERbeta and AR by 
      DHEA metabolites androst-5-ene-3,17-dione (ADIONE), androst-5-ene-3beta,17beta-diol 
      (ADIOL), dihydrotestosterone (DHT), and 5alpha-androstane-3beta,17beta-diol (3beta-Adiol) 
      increased miR-21 transcription. DHEA-induced miR-21 increased cell proliferation 
      and decreased Pdcd4 protein, a bona fide miR-21. Estradiol (E2) inhibited miR-21 
      expression via ERalpha. DHEA increased ERbeta and AR recruitment to the miR-21 promoter 
      within the VMP1/TMEM49 gene, with possible significance in hepatocellular 
      carcinoma.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - Teng, Yun
AU  - Teng Y
AD  - Department of Biochemistry & Molecular Biology, Center for Genetics and Molecular 
      Medicine, University of Louisville School of Medicine, Louisville, KY 40292, USA.
FAU - Litchfield, Lacey M
AU  - Litchfield LM
AD  - Department of Biochemistry & Molecular Biology, Center for Genetics and Molecular 
      Medicine, University of Louisville School of Medicine, Louisville, KY 40292, USA.
FAU - Ivanova, Margarita M
AU  - Ivanova MM
AD  - Department of Biochemistry & Molecular Biology, Center for Genetics and Molecular 
      Medicine, University of Louisville School of Medicine, Louisville, KY 40292, USA.
FAU - Prough, Russell A
AU  - Prough RA
AD  - Department of Biochemistry & Molecular Biology, Center for Genetics and Molecular 
      Medicine, University of Louisville School of Medicine, Louisville, KY 40292, USA.
FAU - Clark, Barbara J
AU  - Clark BJ
AD  - Department of Biochemistry & Molecular Biology, Center for Genetics and Molecular 
      Medicine, University of Louisville School of Medicine, Louisville, KY 40292, USA.
FAU - Klinge, Carolyn M
AU  - Klinge CM
AD  - Department of Biochemistry & Molecular Biology, Center for Genetics and Molecular 
      Medicine, University of Louisville School of Medicine, Louisville, KY 40292, USA. 
      Electronic address: carolyn.klinge@louisville.edu.
LA  - eng
GR  - R01 CA138410/CA/NCI NIH HHS/United States
GR  - T32 ES011564/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140517
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (AR protein, human)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (PDCD4 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Receptors, Androgen)
RN  - 459AG36T1B (Dehydroepiandrosterone)
SB  - IM
MH  - Animals
MH  - Apoptosis Regulatory Proteins/genetics/metabolism
MH  - Base Sequence
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects/genetics
MH  - Dehydroepiandrosterone/*pharmacology
MH  - Estrogen Receptor beta/antagonists & inhibitors/*metabolism
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Gene Knockdown Techniques
MH  - Hep G2 Cells
MH  - Humans
MH  - Male
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding/drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA-Binding Proteins/genetics/metabolism
MH  - Receptors, Androgen/*metabolism
MH  - Transcription, Genetic/*drug effects
MH  - Up-Regulation/drug effects/genetics
PMC - PMC4074919
MID - NIHMS600479
OTO - NOTNLM
OT  - Androgen receptor
OT  - DHEA
OT  - Estrogen receptor
OT  - HepG2 cells
OT  - microRNA
EDAT- 2014/05/23 06:00
MHDA- 2015/02/20 06:00
PMCR- 2015/07/05
CRDT- 2014/05/22 06:00
PHST- 2014/01/17 00:00 [received]
PHST- 2014/05/02 00:00 [revised]
PHST- 2014/05/09 00:00 [accepted]
PHST- 2014/05/22 06:00 [entrez]
PHST- 2014/05/23 06:00 [pubmed]
PHST- 2015/02/20 06:00 [medline]
PHST- 2015/07/05 00:00 [pmc-release]
AID - S0303-7207(14)00149-X [pii]
AID - 10.1016/j.mce.2014.05.007 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2014 Jul 5;392(1-2):23-36. doi: 10.1016/j.mce.2014.05.007. 
      Epub 2014 May 17.