PMID- 24849431
OWN - NLM
STAT- MEDLINE
DCOM- 20150909
LR  - 20220408
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 34
IP  - 5
DP  - 2014 May
TI  - [Advanced oxidation protein products induce epithelial-to-mesenchymal transition 
      in cultured human proximal tubular epithelial cells via oxidative stress].
PG  - 659-63
AB  - OBJECTIVE: To investigate the effects of advanced oxidation protein products 
      (AOPP) on epithelial-to-mesenchymal transition (EMT) in cultured human proximal 
      tubular epithelial cells (HK-2) and explore the mechanism. METHODS: HK-2 cells 
      treated with 50, 100, 200, and 400 microg/ml AOPP or 50 microg/m bovine serum albumin 
      (BSA) for 24 h, or with 200 microg/ml AOPP for 0.5, 1, 3, 6, 12, and 24 h were 
      examined for the protein expression of alpha-SMA and E-cadherin. In cells pretreated 
      with diphenyleneiodonium (DPI) or cytoplasmic superoxide dismutase (C-SOD), the 
      effects of 50 microg/ml BSA and 200 microg/ml AOPP were assessed on the expressions of 
      alpha-SMA and E-cadherin, malondialdehyde (MDA) level, superoxide dismutase (SOD) 
      activity, catalase (CAT) activity, and glutathione peroxidase (GSH-px) activity. 
      RESULTS: AOPP treatment up-regulated alpha-SMA expression and down-regulated 
      E-cadherin expression in a dose- and time-dependent fashion. AOPP exposure of the 
      cells resulted in increased MDA level and lowered activities of SOD, CAT and 
      GSH-PX. DPI and C-SOD partially attenuated the effects of AOPP on alpha-SMA, 
      E-cadherin, MDA, SOD, CAT and GSH-px. CONCLUSION: AOPP can induce EMT in cultured 
      HK-2 cells via oxidative stress, and this effect can be attenuated by inhibiting 
      the activation of NADPH oxidase and using antioxidants to delay the progression 
      of renal interstitial fibrosis.
FAU - Zhang, Jun
AU  - Zhang J
AD  - Department of Nephrology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou 510282, China. E-mail: gzh_zj@qq.com.
FAU - Qiu, Minzi
AU  - Qiu M
FAU - Ma, Yaqiong
AU  - Ma Y
FAU - Bu, Yang
AU  - Bu Y
FAU - Yang, Lei
AU  - Yang L
FAU - Tang, Xun
AU  - Tang X
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Actins)
RN  - 0 (Advanced Oxidation Protein Products)
RN  - 0 (Antigens, CD)
RN  - 0 (Antioxidants)
RN  - 0 (CDH1 protein, human)
RN  - 0 (Cadherins)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.6.3.- (NADPH Oxidases)
SB  - IM
MH  - Actins/metabolism
MH  - *Advanced Oxidation Protein Products
MH  - Antigens, CD
MH  - Antioxidants/metabolism
MH  - Cadherins/metabolism
MH  - Catalase/metabolism
MH  - Cell Line
MH  - Cells, Cultured
MH  - Down-Regulation
MH  - Epithelial Cells/*cytology
MH  - *Epithelial-Mesenchymal Transition
MH  - Glutathione Peroxidase/metabolism
MH  - Humans
MH  - Malondialdehyde/metabolism
MH  - NADPH Oxidases/metabolism
MH  - *Oxidative Stress
MH  - Superoxide Dismutase/metabolism
MH  - Up-Regulation
EDAT- 2014/05/23 06:00
MHDA- 2015/09/10 06:00
CRDT- 2014/05/23 06:00
PHST- 2014/05/23 06:00 [entrez]
PHST- 2014/05/23 06:00 [pubmed]
PHST- 2015/09/10 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2014 May;34(5):659-63.