PMID- 24850147
OWN - NLM
STAT- MEDLINE
DCOM- 20150129
LR  - 20240204
IS  - 0219-1032 (Electronic)
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 37
IP  - 5
DP  - 2014 May
TI  - Increase in hypotonic stress-induced endocytic activity in macrophages via ClC-3.
PG  - 418-25
LID - 10.14348/molcells.2014.0031 [doi]
AB  - Extracellular hypotonic stress can affect cellular function. Whether and how 
      hypotonicity affects immune cell function remains to be elucidated. Macrophages 
      are immune cells that play key roles in adaptive and innate in immune reactions. 
      The purpose of this study was to investigate the role and underlying mechanism of 
      hypotonic stress in the function of bone marrow-derived macrophages (BMDMs). 
      Hypotonic stress increased endocytic activity in BMDMs, but there was no 
      significant change in the expression of CD80, CD86, and MHC class II molecules, 
      nor in the secretion of TNF-alpha or IL-10 by BMDMs. Furthermore, the enhanced 
      endocytic activity of BMDMs triggered by hypotonic stress was significantly 
      inhibited by chloride channel-3 (ClC-3) siRNA. Our findings suggest that 
      hypotonic stress can induce endocytosis in BMDMs and that ClC-3 plays a central 
      role in the endocytic process.
FAU - Yan, Yutao
AU  - Yan Y
AD  - Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 4340030, China.
FAU - Ding, Yu
AU  - Ding Y
AD  - Department of Immunology, Tongji Medical College.
FAU - Ming, Bingxia
AU  - Ming B
FAU - Du, Wenjiao
AU  - Du W
FAU - Kong, Xiaoling
AU  - Kong X
FAU - Tian, Li
AU  - Tian L
FAU - Zheng, Fang
AU  - Zheng F
FAU - Fang, Min
AU  - Fang M
FAU - Tan, Zheng
AU  - Tan Z
FAU - Gong, Feili
AU  - Gong F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140516
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (Chloride Channels)
RN  - 0 (ClC-3 channel)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - B7-1 Antigen/metabolism
MH  - B7-2 Antigen/metabolism
MH  - Cells, Cultured
MH  - Chloride Channels/genetics/*metabolism
MH  - Female
MH  - Gene Expression
MH  - Interleukin-10/metabolism
MH  - Macrophages/*physiology
MH  - Mice, Inbred C57BL
MH  - Osmotic Pressure
MH  - *Pinocytosis
MH  - *Stress, Physiological
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC4044314
EDAT- 2014/05/23 06:00
MHDA- 2015/01/30 06:00
PMCR- 2014/05/16
CRDT- 2014/05/23 06:00
PHST- 2014/02/13 00:00 [received]
PHST- 2014/04/01 00:00 [accepted]
PHST- 2014/05/23 06:00 [entrez]
PHST- 2014/05/23 06:00 [pubmed]
PHST- 2015/01/30 06:00 [medline]
PHST- 2014/05/16 00:00 [pmc-release]
AID - S1016-8478(23)05326-8 [pii]
AID - molcell-37-5-418-9 [pii]
AID - 10.14348/molcells.2014.0031 [doi]
PST - ppublish
SO  - Mol Cells. 2014 May;37(5):418-25. doi: 10.14348/molcells.2014.0031. Epub 2014 May 
      16.