PMID- 24850305
OWN - NLM
STAT- MEDLINE
DCOM- 20150914
LR  - 20140627
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 46
IP  - 7
DP  - 2014 Jul
TI  - Myeloma cells resistance to NK cell lysis mainly involves an HLA class 
      I-dependent mechanism.
PG  - 597-604
LID - 10.1093/abbs/gmu041 [doi]
AB  - The anti-multiple myeloma (MM) potential of natural killer (NK) cells has been of 
      rising interest in recent years. However, the molecular mechanism of NK cell 
      cytotoxicity to myeloma cells remains unclear. In the present study, we 
      investigated the expressions of human leukocyte antigen (HLA) class I and HLA-G 
      in patient myeloma cells, and determined their relevance in patient tumor-cell 
      susceptibility to NK cell cytotoxicity. Our results showed that patient myeloma 
      cells (n = 12) were relatively resistant to NK-92 cell lysis, compared with 
      myeloma cell lines (n = 7, P < 0.01). Gene expression profiling and flow 
      cytometry analysis showed that both mRNA and protein of HLA class I were highly 
      expressed in 12 patient myeloma cells. Interestingly, no or low HLA-G surface 
      expression was detected, although multiple HLA-G transcripts were detected in 
      these myeloma cells. NK cell function assay showed that down-regulating HLA class 
      I expression on patient cells by acid treatment significantly increased the 
      susceptibility of MM cells to NK-mediated lysis. Furthermore, we found that the 
      blocking of membrane-bound HLA class I rather than HLA-G using antibodies on 
      myeloma samples markedly increased their susceptibility to NK-mediated killing. 
      These results demonstrated that the resistance of patient MM cells to NK lysis 
      mainly involves an HLA class I-dependent mechanism, suggesting that HLA class I 
      may be involved in protecting MM cells from NK-mediated attack and contribute to 
      their immune escape in vivo.
CI  - (c) The Author 2014. Published by ABBS Editorial Office in association with Oxford 
      University Press on behalf of the Institute of Biochemistry and Cell Biology, 
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
FAU - Gao, Minjie
AU  - Gao M
AD  - Department of Hematology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, China.
FAU - Gao, Lu
AU  - Gao L
AD  - Department of Hematology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, China.
FAU - Yang, Guang
AU  - Yang G
AD  - Department of Hematology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, China.
FAU - Tao, Yi
AU  - Tao Y
AD  - Department of Hematology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, China.
FAU - Hou, Jun
AU  - Hou J
AD  - Department of Hematology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, China.
FAU - Xu, Hongwei
AU  - Xu H
AD  - Department of Internal Medicine, University of Iowa, Carver College of Medicine, 
      Iowa City, IA 52242, USA.
FAU - Hu, Xiaojing
AU  - Hu X
AD  - Department of Hematology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, China.
FAU - Han, Ying
AU  - Han Y
AD  - Department of Hematology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, China.
FAU - Zhang, Qianqiao
AU  - Zhang Q
AD  - Department of Hematology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, China.
FAU - Zhan, Fenghuang
AU  - Zhan F
AD  - Department of Internal Medicine, University of Iowa, Carver College of Medicine, 
      Iowa City, IA 52242, USA.
FAU - Wu, Xiaosong
AU  - Wu X
AD  - Department of Hematology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, China shijumei@hotmail.com 
      wuxiaosong@gmail.com.
FAU - Shi, Jumei
AU  - Shi J
AD  - Department of Hematology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, China shijumei@hotmail.com 
      wuxiaosong@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140521
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cytotoxicity, Immunologic
MH  - Down-Regulation
MH  - Flow Cytometry
MH  - Gene Expression Profiling
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - Killer Cells, Natural/*immunology
MH  - Multiple Myeloma/genetics/*immunology/pathology
OTO - NOTNLM
OT  - HLA class I
OT  - cytotoxicity
OT  - myeloma
OT  - natural killer cells
EDAT- 2014/05/23 06:00
MHDA- 2015/09/15 06:00
CRDT- 2014/05/23 06:00
PHST- 2014/05/23 06:00 [entrez]
PHST- 2014/05/23 06:00 [pubmed]
PHST- 2015/09/15 06:00 [medline]
AID - gmu041 [pii]
AID - 10.1093/abbs/gmu041 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2014 Jul;46(7):597-604. doi: 
      10.1093/abbs/gmu041. Epub 2014 May 21.