PMID- 2485200
OWN - NLM
STAT- MEDLINE
DCOM- 19920511
LR  - 20191029
IS  - 0952-0600 (Print)
IS  - 0952-0600 (Linking)
VI  - 2
IP  - 3
DP  - 1989
TI  - Secretion of thromboxane B2 and prostaglandin E2 by guinea-pig lung cells 
      isolated by centrifugal elutriation and by macrophages obtained by 
      bronchoalveolar lavage.
PG  - 137-45
AB  - Guinea-pig lung cells were partially purified by an elutriation technique after 
      enzymatic digestion of the lung. Eight fractions were obtained and the cell 
      content of each fraction was analysed by staining and by electron microscopy. 
      After stimulation with selected agonists, the secretion of eicosanoids has been 
      measured in the supernatants of each elutriation fraction and in the supernatants 
      of alveolar macrophages obtained by bronchoalveolar lavage. The cell response was 
      characterised by a marked secretion of thromboxane B2 (TXB2) and prostaglandin E2 
      (PGE2) from the alveolar macrophages and a more discrete secretion of TXB2 from 
      the cells of the elutriated fractions. The secretion of TXB2 varied in the cell 
      fractions as a function of the cellular composition and the agonist used. 
      Platelet activating factor (PAF) and ethoxy PAF, phorbol myristate acetate (PMA) 
      and ionophore A-23187 stimulated most cell types although PMA produced a more 
      important stimulation of the fractions containing pneumocytes and eosinophils, 
      and ionophore A-23187 appeared to stimulate Foa-Kurloff cells. The chemotactic 
      peptide fMLP stimulated markedly the fractions containing the inflammatory cells 
      (mast cells, macrophages, neutrophils) and endothelial cells. Pretreatment of the 
      cells with cytochalasin B potentiated the release of TXB2 and PGE2 by macrophages 
      following stimulation with PAF, leukotriene D4 (LTD4) and 
      formyl-methionyl-leucyl-phenylalanine (fMLP) but not by mixed lung cells or by 
      elutriation fractions. In conclusion, the recovery of a large number of partially 
      purified lung cell types was made possible by mild protease digestion and 
      elutriation.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Pele, J P
AU  - Pele JP
AD  - Department of Pharmacology, Faculty of Medicine, University of Sherbrooke, 
      Canada.
FAU - Robidoux, C
AU  - Robidoux C
FAU - Sirois, P
AU  - Sirois P
LA  - eng
PT  - Journal Article
PL  - England
TA  - Pulm Pharmacol
JT  - Pulmonary pharmacology
JID - 9007551
RN  - 54397-85-2 (Thromboxane B2)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/*cytology
MH  - Centrifugation
MH  - Cytological Techniques
MH  - Dinoprostone/*metabolism
MH  - Guinea Pigs
MH  - In Vitro Techniques
MH  - Lung/cytology/*metabolism
MH  - Macrophages, Alveolar/*metabolism
MH  - Microscopy, Electron
MH  - Staining and Labeling
MH  - Stimulation, Chemical
MH  - Thromboxane B2/*metabolism
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 0952-0600(89)90038-0 [pii]
AID - 10.1016/0952-0600(89)90038-0 [doi]
PST - ppublish
SO  - Pulm Pharmacol. 1989;2(3):137-45. doi: 10.1016/0952-0600(89)90038-0.