PMID- 24852167
OWN - NLM
STAT- MEDLINE
DCOM- 20151109
LR  - 20211021
IS  - 1539-7262 (Electronic)
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 55
IP  - 9
DP  - 2014 Sep
TI  - Spatiotemporal lipid profiling during early embryo development of Xenopus laevis 
      using dynamic ToF-SIMS imaging.
PG  - 1970-80
LID - 10.1194/jlr.D048660 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging has been used 
      for the direct analysis of single intact Xenopus laevis embryo surfaces, locating 
      multiple lipids during fertilization and the early embryo development stages with 
      subcellular lateral resolution ( approximately 4 mum). The method avoids the complicated sample 
      preparation for lipid analysis of the embryos, which requires selective chemical 
      extraction of a pool of samples and chromatographic separation, while preserving 
      the spatial distribution of biological species. The results show ToF-SIMS is 
      capable of profiling multiple components (e.g., glycerophosphocholine, SM, 
      cholesterol, vitamin E, diacylglycerol, and triacylglycerol) in a single X. 
      laevis embryo. We observe lipid remodeling during fertilization and early embryo 
      development via time course sampling. The study also reveals the lipid 
      distribution on the gamete fusion site. The methodology used in the study opens 
      the possibility of studying developmental biology using high resolution imaging 
      MS and of understanding the functional role of the biological molecules.
CI  - Copyright (c) 2014 by the American Society for Biochemistry and Molecular Biology, 
      Inc.
FAU - Tian, Hua
AU  - Tian H
AD  - Manchester Institute of Biotechnology, School of Chemical Engineering and 
      Analytical Science, University of Manchester, Manchester, UK.
FAU - Fletcher, John S
AU  - Fletcher JS
AD  - Manchester Institute of Biotechnology, School of Chemical Engineering and 
      Analytical Science, University of Manchester, Manchester, UK.
FAU - Thuret, Raphael
AU  - Thuret R
AD  - Faculty of Life Science, University of Manchester, Manchester, UK.
FAU - Henderson, Alex
AU  - Henderson A
AD  - Manchester Institute of Biotechnology, School of Chemical Engineering and 
      Analytical Science, University of Manchester, Manchester, UK.
FAU - Papalopulu, Nancy
AU  - Papalopulu N
AD  - Faculty of Life Science, University of Manchester, Manchester, UK.
FAU - Vickerman, John C
AU  - Vickerman JC
AD  - Manchester Institute of Biotechnology, School of Chemical Engineering and 
      Analytical Science, University of Manchester, Manchester, UK.
FAU - Lockyer, Nicholas P
AU  - Lockyer NP
AD  - Manchester Institute of Biotechnology, School of Chemistry, University of 
      Manchester, Manchester, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140522
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
SB  - IM
MH  - Animals
MH  - Embryonic Development
MH  - Female
MH  - Imaging, Three-Dimensional
MH  - *Lipid Metabolism
MH  - Male
MH  - Mass Spectrometry
MH  - Xenopus laevis/embryology/*metabolism
MH  - Zygote/metabolism
PMC - PMC4617352
OTO - NOTNLM
OT  - embryo
OT  - mass spectrometry imaging
OT  - oocyte
OT  - time-of-flight secondary ion mass spectrometry
EDAT- 2014/05/24 06:00
MHDA- 2015/11/10 06:00
PMCR- 2015/09/01
CRDT- 2014/05/24 06:00
PHST- 2014/05/24 06:00 [entrez]
PHST- 2014/05/24 06:00 [pubmed]
PHST- 2015/11/10 06:00 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - S0022-2275(20)35672-8 [pii]
AID - d048660 [pii]
AID - 10.1194/jlr.D048660 [doi]
PST - ppublish
SO  - J Lipid Res. 2014 Sep;55(9):1970-80. doi: 10.1194/jlr.D048660. Epub 2014 May 22.