PMID- 24852305
OWN - NLM
STAT- MEDLINE
DCOM- 20150630
LR  - 20140917
IS  - 1421-976X (Electronic)
IS  - 0008-6568 (Linking)
VI  - 48
IP  - 5
DP  - 2014
TI  - Molecular studies of the structural ecology of natural occlusal caries.
PG  - 451-60
LID - 10.1159/000357920 [doi]
AB  - Microbiological studies of occlusal dental biofilms have hitherto been hampered 
      by inaccessibility to the sampling site and demolition of the original biofilm 
      architecture. This study shows for the first time the spatial distribution of 
      bacterial taxa in vivo at various stages of occlusal caries, applying a molecular 
      methodology involving preparation of embedded hard dental tissue slices for 
      fluorescence in situ hybridization (FISH) and confocal microscopy. Eleven freshly 
      extracted teeth were classified according to their occlusal caries status. The 
      teeth were fixed, embedded, sectioned and decalcified before FISH was performed 
      using oligonucleotide probes for selected abundant species/genera associated with 
      occlusal caries including Streptococcus, Actinomyces, Veillonella, Fusobacterium, 
      Lactobacillus and Bifidobacterium. The sites showed distinct differences in the 
      bacterial composition between different ecological niches in occlusal caries. 
      Biofilm observed along the entrance of fissures showed an inner layer of 
      microorganisms organized in palisades often identified as Actinomyces, covered by 
      a more loosely structured bacterial layer consisting of diverse genera, similar 
      to supragingival biofilm. Biofilm within the fissure proper seemed less 
      metabolically active, as judged by low fluorescence signal intensity and presence 
      of material of non-bacterial origin. Bacterial invasion (often Lactobacillus and 
      Bifidobacterium spp.) into the dentinal tubules was seen only at advanced stages 
      of caries with manifest cavity formation. It is concluded that the molecular 
      methodology represents a valuable supplement to previous methods for the study of 
      microbial ecology in caries by allowing analysis of the structural composition of 
      the undisturbed biofilm in caries lesions in vivo.
CI  - (c) 2014 S. Karger AG, Basel.
FAU - Dige, Irene
AU  - Dige I
AD  - Section for Dental Pathology, Operative Dentistry and Endodontics, Department of 
      Dentistry, Aarhus University, Aarhus, Denmark.
FAU - Gronkjaer, Lene
AU  - Gronkjaer L
FAU - Nyvad, Bente
AU  - Nyvad B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140515
PL  - Switzerland
TA  - Caries Res
JT  - Caries research
JID - 0103374
SB  - IM
MH  - Actinomyces/cytology
MH  - Bacteria/classification/*cytology
MH  - Bifidobacterium/cytology
MH  - *Biofilms
MH  - Dental Caries/*microbiology
MH  - Dental Enamel/microbiology
MH  - Dental Fissures/microbiology
MH  - Dentin/microbiology/ultrastructure
MH  - Fusobacterium/cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lactobacillus/cytology
MH  - Microbial Consortia
MH  - Microscopy, Confocal
MH  - Molecular Biology
MH  - Plastic Embedding
MH  - Streptococcus/classification/cytology
MH  - Streptococcus mitis/cytology
MH  - Streptococcus mutans/cytology
MH  - Veillonella/cytology
EDAT- 2014/05/24 06:00
MHDA- 2015/07/01 06:00
CRDT- 2014/05/24 06:00
PHST- 2013/10/08 00:00 [received]
PHST- 2013/12/08 00:00 [accepted]
PHST- 2014/05/24 06:00 [entrez]
PHST- 2014/05/24 06:00 [pubmed]
PHST- 2015/07/01 06:00 [medline]
AID - 000357920 [pii]
AID - 10.1159/000357920 [doi]
PST - ppublish
SO  - Caries Res. 2014;48(5):451-60. doi: 10.1159/000357920. Epub 2014 May 15.