PMID- 2485257
OWN - NLM
STAT- MEDLINE
DCOM- 19940404
LR  - 20131121
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 3
IP  - 6
DP  - 1988 Dec
TI  - A selectable temperature-sensitive v-src Moloney retrovirus.
PG  - 687-9
AB  - We have cloned the v-src gene of ts339B77 RSV into a new Moloney-based retroviral 
      expression vector (YN). In the resulting construct (ts339YNsrc), the ts339 gene 
      is transcribed from the viral LTR, while a selectable resitance marker, the Tn5 
      neomycin phosphotransferase (neor) gene, is transcribed from an internal 
      thymidine kinase (Tk) promoter. G418 resistance and focus formation were induced 
      in NIH3T3 cells at comparable efficiencies within the permissive temperature 
      range (33-37 degrees C). At 39 degrees, on the other hand, focus induction was 
      reduced 15-fold with no corresponding decrease in expression of G418 resistance. 
      In cells infected with ts339YNsrc, phosphoproteins were elevated and similar in 
      pattern on SDS PAGE regardless of whether the cells were grown at the permissive 
      or restrictive temperature. The ts339YNsrc virus will be useful for the study of 
      effects of v-src expression, and may also be of help in identifying relevant 
      substrates of the v-src product.
FAU - Brady, G
AU  - Brady G
AD  - Ontario Cancer Institute, Toronto, Canada.
FAU - Williams, R L
AU  - Williams RL
FAU - Nordstrom, K
AU  - Nordstrom K
FAU - Vennstrom, B
AU  - Vennstrom B
FAU - Iscove, N N
AU  - Iscove NN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Oncogene Protein pp60(v-src))
SB  - IM
GS  - ts339
GS  - v-src
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Line
MH  - Chickens
MH  - *Genes, src
MH  - Mice
MH  - Moloney murine leukemia virus/*genetics/isolation & purification
MH  - Mutagenesis
MH  - Oncogene Protein pp60(v-src)/*biosynthesis/metabolism
MH  - Phosphotyrosine
MH  - Protein-Tyrosine Kinases/biosynthesis/metabolism
MH  - Restriction Mapping
MH  - Temperature
MH  - Transfection
MH  - Tyrosine/analogs & derivatives/analysis
EDAT- 1988/12/01 00:00
MHDA- 1988/12/01 00:01
CRDT- 1988/12/01 00:00
PHST- 1988/12/01 00:00 [pubmed]
PHST- 1988/12/01 00:01 [medline]
PHST- 1988/12/01 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1988 Dec;3(6):687-9.