PMID- 24854856
OWN - NLM
STAT- MEDLINE
DCOM- 20140729
LR  - 20220409
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 55
IP  - 6
DP  - 2014 May 22
TI  - Monitoring retinal morphologic and functional changes in mice following optic 
      nerve crush.
PG  - 3766-74
LID - 10.1167/iovs.14-13895 [doi]
AB  - PURPOSE: We characterized the morphologic and functional changes in optic nerve 
      crushed mice and evaluated electroretinogram (ERG) responses as tools to monitor 
      retinal ganglion cell (RGC) dysfunction. METHODS: We performed optic nerve crush 
      (ONC) unilaterally in adult BALB/cJ mice. The neuronal loss in the RGC layer 
      (GCL) and superior colliculus (SC) was determined by Nissl staining. Retinal 
      thickness was assessed by spectral-domain optical coherence tomography (SD-OCT) 
      imaging. Retinal function was determined by pattern ERG and full-field flash ERG. 
      Responses of pattern ERG, positive scotopic threshold response (pSTR), scotopic 
      oscillatory potentials (OPs), and photopic negative response (PhNR) were 
      analyzed. RESULTS: The ONC induced progressive neuronal loss in GCL and 
      contralateral SC starting from 7 and 28 days following ONC, respectively. A 
      linear correlation was observed between combined thickness of nerve fiber layer 
      (NFL), GCL, and inner plexiform layer (IPL) imaged by SD-OCT and cell numbers in 
      GCL. Only half of the normal BALB/cJ mice exhibited pattern ERG response, which 
      was smaller and later compared to C57BL/6J mice. The ONC reduced pattern ERG and 
      pSTR, but the reduction of pattern ERG was earlier than pSTR, preceding the 
      anatomical cell loss in the GCL. The PhNR and scotopic OPs were not affected by 
      ONC. CONCLUSIONS: The SD-OCT and ERG can be used to monitor noninvasively retinal 
      morphologic and functional changes induced by ONC. Pattern ERG and pSTR are able 
      to detect early RGC dysfunction, but pattern ERG exhibits higher sensitivity. Our 
      results support the use of these tools in studies using the mouse ONC model.
CI  - Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
FAU - Liu, Yang
AU  - Liu Y
AD  - North Texas Eye Research Institute and Department of Cell Biology and Immunology, 
      University of North Texas Health Science Center, Fort Worth, Texas, United 
      States.
FAU - McDowell, Colleen M
AU  - McDowell CM
AD  - North Texas Eye Research Institute and Department of Cell Biology and Immunology, 
      University of North Texas Health Science Center, Fort Worth, Texas, United 
      States.
FAU - Zhang, Zhang
AU  - Zhang Z
AD  - North Texas Eye Research Institute and Department of Cell Biology and Immunology, 
      University of North Texas Health Science Center, Fort Worth, Texas, United 
      States.
FAU - Tebow, Holly E
AU  - Tebow HE
AD  - North Texas Eye Research Institute and Department of Cell Biology and Immunology, 
      University of North Texas Health Science Center, Fort Worth, Texas, United 
      States.
FAU - Wordinger, Robert J
AU  - Wordinger RJ
AD  - North Texas Eye Research Institute and Department of Cell Biology and Immunology, 
      University of North Texas Health Science Center, Fort Worth, Texas, United 
      States.
FAU - Clark, Abbot F
AU  - Clark AF
AD  - North Texas Eye Research Institute and Department of Cell Biology and Immunology, 
      University of North Texas Health Science Center, Fort Worth, Texas, United 
      States.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140522
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Disease Models, Animal
MH  - Electroretinography
MH  - Female
MH  - Follow-Up Studies
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Monitoring, Physiologic/*methods
MH  - Nerve Crush/*adverse effects
MH  - Optic Nerve/pathology/*physiopathology
MH  - Optic Nerve Injuries/complications/diagnosis/*physiopathology
MH  - Retina/*pathology/physiopathology
MH  - Retinal Ganglion Cells/physiology
MH  - Scotoma/diagnosis/etiology/*physiopathology
MH  - Tomography, Optical Coherence
OTO - NOTNLM
OT  - electroretinogram
OT  - optic nerve crush
OT  - retinal ganglion cells
EDAT- 2014/05/24 06:00
MHDA- 2014/07/30 06:00
CRDT- 2014/05/24 06:00
PHST- 2014/05/24 06:00 [entrez]
PHST- 2014/05/24 06:00 [pubmed]
PHST- 2014/07/30 06:00 [medline]
AID - iovs.14-13895 [pii]
AID - 10.1167/iovs.14-13895 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2014 May 22;55(6):3766-74. doi: 10.1167/iovs.14-13895.