PMID- 24856853
OWN - NLM
STAT- MEDLINE
DCOM- 20140922
LR  - 20151119
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 45
IP  - 8
DP  - 2014 Aug
TI  - Diagnostic utility of NCOA2 fluorescence in situ hybridization and Stat6 
      immunohistochemistry staining for soft tissue angiofibroma and morphologically 
      similar fibrovascular tumors.
PG  - 1588-96
LID - S0046-8177(14)00149-X [pii]
LID - 10.1016/j.humpath.2013.12.022 [doi]
AB  - Soft tissue angiofibroma (STA), a recently suggested new histologic entity, is a 
      benign fibrovascular soft tissue tumor composed of bland spindle-shaped tumor 
      cells with abundant collagenous to myxoid stroma and branching small vessels. The 
      lesion has a characteristic AHRR-NCOA2 fusion gene derived from chromosomal 
      translocation of t(5;8)(p15;q13). However, morphologically similar tumors 
      containing abundant fibrovascular and myxoid stroma can complicate diagnosis. We 
      designed an original DNA probe for detecting NCOA2 split signals on fluorescence 
      in situ hybridization (FISH) and estimated its utility with 20 fibrovascular 
      tumors: 4 each of STAs, solitary fibrous tumors (SFTs), and cellular 
      angiofibromas and 3 each of low-grade myxofibrosarcomas, myxoid liposarcomas, and 
      low-grade fibromyxoid sarcomas. We also performed FISH for 13q14 deletion and 
      immunohistochemistry (IHC) staining for estrogen receptor, progesterone receptor, 
      retinoblastoma protein, and MUC-4 expression. Furthermore, IHC for Stat6 was 
      conducted in the 20 cases analyzed by FISH and in an additional 26 SFTs. We found 
      moderate to strong nuclear Stat6 expression in all SFTs but no expression in the 
      other tumors. Both estrogen receptor and progesterone receptor expressions were 
      observed in STAs, SFTs, and cellular angiofibromas. Expression of retinoblastoma 
      protein was found in less than 10% of cells in all tumor types except myxoid 
      liposarcoma. The low-grade fibromyxoid sarcomas were strongly positive for MUC-4. 
      All STAs showed NCOA2 split signals on FISH. All tumors, regardless of histologic 
      type, had 13q14 deletion. The NCOA2 FISH technique is a practical method for 
      confirming STA diagnosis. The combination of NCOA2 FISH and Stat6 IHC proved 
      effective for the differential diagnosis of STA, even when using small biopsy 
      specimens.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Sugita, Shintaro
AU  - Sugita S
AD  - Department of Surgical Pathology, Sapporo Medical University School of Medicine, 
      Sapporo 060-8543, Japan.
FAU - Aoyama, Tomoyuki
AU  - Aoyama T
AD  - Department of Surgical Pathology, Sapporo Medical University School of Medicine, 
      Sapporo 060-8543, Japan.
FAU - Kondo, Kei
AU  - Kondo K
AD  - Department of Surgical Pathology, Sapporo Medical University School of Medicine, 
      Sapporo 060-8543, Japan.
FAU - Keira, Yoshiko
AU  - Keira Y
AD  - Department of Surgical Pathology, Sapporo Medical University School of Medicine, 
      Sapporo 060-8543, Japan.
FAU - Ogino, Jiro
AU  - Ogino J
AD  - Department of Surgical Pathology, Sapporo Medical University School of Medicine, 
      Sapporo 060-8543, Japan.
FAU - Nakanishi, Katsuya
AU  - Nakanishi K
AD  - Department of Surgical Pathology, Sapporo Medical University School of Medicine, 
      Sapporo 060-8543, Japan.
FAU - Kaya, Mitsunori
AU  - Kaya M
AD  - Department of Orthopedic Surgery, Sapporo Medical University School of Medicine, 
      Sapporo 060-8543, Japan.
FAU - Emori, Makoto
AU  - Emori M
AD  - Department of Orthopedic Surgery, Sapporo Medical University School of Medicine, 
      Sapporo 060-8543, Japan.
FAU - Tsukahara, Tomohide
AU  - Tsukahara T
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo 
      060-8543, Japan.
FAU - Nakajima, Hisaya
AU  - Nakajima H
AD  - Department of Orthopedic Surgery, St Marianna University School of Medicine, 
      Kawasaki 216-8511, Japan.
FAU - Takagi, Masayuki
AU  - Takagi M
AD  - Department of Diagnostic Pathology, St Marianna University School of Medicine, 
      Kawasaki 216-8511, Japan.
FAU - Hasegawa, Tadashi
AU  - Hasegawa T
AD  - Department of Surgical Pathology, Sapporo Medical University School of Medicine, 
      Sapporo 060-8543, Japan. Electronic address: hasetada@sapmed.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20140418
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (NCOA2 protein, human)
RN  - 0 (Nuclear Receptor Coactivator 2)
RN  - 0 (STAT6 Transcription Factor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Angiofibroma/*diagnosis/genetics/metabolism
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 13
MH  - Diagnosis, Differential
MH  - Female
MH  - Fibrosarcoma/diagnosis/genetics/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Liposarcoma, Myxoid/diagnosis/genetics/metabolism
MH  - Male
MH  - Middle Aged
MH  - Nuclear Receptor Coactivator 2/genetics/*metabolism
MH  - STAT6 Transcription Factor/*metabolism
MH  - Soft Tissue Neoplasms/*diagnosis/genetics/metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - Immunohistochemistry
OT  - NCOA2 split signal
OT  - Soft tissue angiofibroma
OT  - Stat6
EDAT- 2014/05/27 06:00
MHDA- 2014/09/23 06:00
CRDT- 2014/05/27 06:00
PHST- 2013/09/19 00:00 [received]
PHST- 2013/12/18 00:00 [revised]
PHST- 2013/12/27 00:00 [accepted]
PHST- 2014/05/27 06:00 [entrez]
PHST- 2014/05/27 06:00 [pubmed]
PHST- 2014/09/23 06:00 [medline]
AID - S0046-8177(14)00149-X [pii]
AID - 10.1016/j.humpath.2013.12.022 [doi]
PST - ppublish
SO  - Hum Pathol. 2014 Aug;45(8):1588-96. doi: 10.1016/j.humpath.2013.12.022. Epub 2014 
      Apr 18.