PMID- 24857506
OWN - NLM
STAT- MEDLINE
DCOM- 20150119
LR  - 20191210
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 40
DP  - 2014 Jul 1
TI  - In vitro and in vivo evaluation of a new nanocomposite, containing high density 
      polyethylene, tricalcium phosphate, hydroxyapatite, and magnesium oxide 
      nanoparticles.
PG  - 382-8
LID - S0928-4931(14)00210-0 [pii]
LID - 10.1016/j.msec.2014.04.018 [doi]
AB  - In this study, a new nanocomposite, which contained high density polyethylene 
      (HDPE), tricalcium phosphate (Ca3(PO4)2) nanoparticles (TCP NPs), hydroxyapatite 
      nanoparticles (HA NPs), and magnesium oxide nanoparticles (MgO NPs) was prepared. 
      As in vitro experiment, human osteoblasts (HOB) cells were exposed to pristine 
      HDPE and its nanocomposite for a period of 1, 4, and 7 days at 37  degrees C, and then 
      different assays were carried out, including osteoblast cell proliferation, 
      Trypan blue staining, cell viability, alkaline phosphatase (ALP), and cell 
      adhesion. Antibacterial property of pristine HDPE and its nanocomposite was 
      evaluated, and also their mechanical properties were measured after 2 and 4 
      months. As in vivo experiment, pristine HDPE and its nanocomposite were 
      separately implanted on calvarium bone of rabbits, and tissue inflammation and 
      osteogenesis were investigated after 2, 4, and 6 months. In case of HOB cells 
      treated with HDPE or nanocomposite, as incubation time was increased, cell 
      proliferation, live/dead ratio, and cell viability were decreased. But, the ALP 
      activity and cell adhesion of HOB cells which treated with nanocomposite were 
      raised after increase of incubation time. This study demonstrated that although 
      the mechanical properties of nanocomposite were similar to HDPE sheet, but their 
      antibacterial property was not similar. The in vivo experiment showed that both 
      pristine HDPE and its nanocomposite had same inflammation responses. 
      Interestingly, osteogenesis was observed after 2 months at bone/nanocomposite 
      interface, and was highly increased after 4 and 6 months. It must be noted that 
      such pattern was not seen at bone/HDPE interface.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Pourdanesh, Fereydoun
AU  - Pourdanesh F
AD  - Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti 
      University of Medical Sciences, Tehran 8916733754, Iran.
FAU - Jebali, Ali
AU  - Jebali A
AD  - Department of Medical Physics and Biomedical Engineering, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran. Electronic address: 
      alijebal2011@gmail.com.
FAU - Hekmatimoghaddam, Seyedhossein
AU  - Hekmatimoghaddam S
AD  - Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi 
      University of Medical Sciences, Yazd, Iran.
FAU - Allaveisie, Azra
AU  - Allaveisie A
AD  - Department of Genetics, Research and Clinical Center for Infertility, Shahid 
      Sadoughi University of Medical Sciences, Yazd, Iran.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140415
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bone Substitutes)
RN  - 0 (Calcium Phosphates)
RN  - 3A3U0GI71G (Magnesium Oxide)
RN  - 9002-88-4 (Polyethylene)
RN  - 91D9GV0Z28 (Durapatite)
RN  - K4C08XP666 (tricalcium phosphate)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/chemistry/pharmacology
MH  - Bone Substitutes/chemistry/pharmacology
MH  - Calcium Phosphates/chemistry
MH  - Cell Adhesion/*drug effects
MH  - Cell Line
MH  - Cell Proliferation/*drug effects
MH  - Cell Survival/drug effects
MH  - Durapatite/chemistry
MH  - Escherichia coli/drug effects
MH  - Humans
MH  - Inflammation/etiology
MH  - Magnesium Oxide/chemistry
MH  - Nanocomposites/chemistry/*toxicity
MH  - Nanoparticles/*chemistry
MH  - Osteogenesis/drug effects
MH  - Polyethylene/chemistry
MH  - Pseudomonas aeruginosa/drug effects
MH  - Rabbits
MH  - Staphylococcus aureus/drug effects
MH  - Temperature
MH  - Time Factors
OTO - NOTNLM
OT  - High density polyethylene
OT  - Hydroxyapatite
OT  - Magnesium oxide
OT  - Nanoparticles
OT  - Tricalcium phosphate
EDAT- 2014/05/27 06:00
MHDA- 2015/01/20 06:00
CRDT- 2014/05/27 06:00
PHST- 2013/07/18 00:00 [received]
PHST- 2014/01/22 00:00 [revised]
PHST- 2014/04/07 00:00 [accepted]
PHST- 2014/05/27 06:00 [entrez]
PHST- 2014/05/27 06:00 [pubmed]
PHST- 2015/01/20 06:00 [medline]
AID - S0928-4931(14)00210-0 [pii]
AID - 10.1016/j.msec.2014.04.018 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2014 Jul 1;40:382-8. doi: 
      10.1016/j.msec.2014.04.018. Epub 2014 Apr 15.