PMID- 24857910
OWN - NLM
STAT- MEDLINE
DCOM- 20150106
LR  - 20211021
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 15
IP  - 5
DP  - 2014 May 5
TI  - Huperzine A ameliorates cognitive deficits in streptozotocin-induced diabetic 
      rats.
PG  - 7667-83
LID - 10.3390/ijms15057667 [doi]
AB  - The present study was designed to probe the effects of Huperzine A (HupA) on 
      diabetes-associated cognitive decline (DACD) using a streptozotocin 
      (STZ)-injected rat model. Diabetic rats were treated with HupA (0.05 and 0.1 
      mg/kg) for seven weeks. Memory functions were evaluated by the water maze test. 
      Nissl staining was selected for detecting neuronal loss. Protein and mRNA levels 
      of brain-derived neurotrophic factor (BDNF) were analyzed by ELISA and real-time 
      PCR, respectively. The activities of choline acetylase (ChAT), 
      Acetylcholinesterase (AChE), malondialdehyde (MDA), superoxide dismutase (SOD), 
      glutathione peroxidase (GSH-Px), catalase (CAT), NF-kappaB p65 unit, TNF-alpha, IL-1beta, 
      IL-6 and caspase-3 were measured using corresponding kits. After seven weeks, 
      diabetic rats exhibited remarkable reductions in: body weight, percentage of time 
      spent in target quadrant, number of times crossing the platform, ChAT and BDNF 
      levels, SOD, GSH-Px and CAT accompanied with increases in neuronal damage, plasma 
      glucose levels, escape latency, mean path length, AChE, MDA level as well as CAT, 
      NF-kappaB p65 unit, TNF-alpha, IL-1beta, IL-6 and caspase-3 in cerebral cortex and 
      hippocampus. Supplementation with HupA significantly and dose-dependently 
      reversed the corresponding values in diabetes. It is concluded that HupA 
      ameliorates DACD via modulating BDNF, oxidative stress, inflammation and 
      apoptosis.
FAU - Mao, Xiao-Yuan
AU  - Mao XY
AD  - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, 
      Central South University, Changsha 410078, China. maoxiaoyuan2011@163.com.
FAU - Cao, Dan-Feng
AU  - Cao DF
AD  - Department of Genetics, Institute of Medical Biology, Chinese Academy of Medical 
      Sciences & Peking Union Medical College, Kunming 650118, China. 
      danfengcao2014@163.com.
FAU - Li, Xi
AU  - Li X
AD  - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, 
      Central South University, Changsha 410078, China. xilidr@163.com.
FAU - Yin, Ji-Ye
AU  - Yin JY
AD  - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, 
      Central South University, Changsha 410078, China. jiyeyindr2014@163.com.
FAU - Wang, Zhi-Bin
AU  - Wang ZB
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China. 
      zhibinwang2014@163.com.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, 
      Central South University, Changsha 410078, China. yingzhang2014@163.com.
FAU - Mao, Chen-Xue
AU  - Mao CX
AD  - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, 
      Central South University, Changsha 410078, China. chenxuemao2014@163.com.
FAU - Zhou, Hong-Hao
AU  - Zhou HH
AD  - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, 
      Central South University, Changsha 410078, China. honghaozhoudr@163.com.
FAU - Liu, Zhao-Qian
AU  - Liu ZQ
AD  - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, 
      Central South University, Changsha 410078, China. liuzhaoqian63@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140505
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Alkaloids)
RN  - 0 (Blood Glucose)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cytokines)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sesquiterpenes)
RN  - 0111871I23 (huperzine A)
RN  - 5W494URQ81 (Streptozocin)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Alkaloids/chemistry/*pharmacology/therapeutic use
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Body Weight/drug effects
MH  - Brain-Derived Neurotrophic Factor/analysis/genetics/metabolism
MH  - Caspase 3/metabolism
MH  - Cognition Disorders/complications/drug therapy
MH  - Cytokines/metabolism
MH  - Diabetes Mellitus, Experimental/complications/metabolism/pathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Hippocampus/drug effects/metabolism
MH  - Male
MH  - Memory/*drug effects
MH  - Neuroprotective Agents/chemistry/*pharmacology/therapeutic use
MH  - Oxidative Stress/drug effects
MH  - Oxidoreductases/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sesquiterpenes/chemistry/*pharmacology/therapeutic use
MH  - Streptozocin/toxicity
PMC - PMC4057698
EDAT- 2014/05/27 06:00
MHDA- 2015/01/07 06:00
PMCR- 2014/05/01
CRDT- 2014/05/27 06:00
PHST- 2014/02/24 00:00 [received]
PHST- 2014/04/15 00:00 [revised]
PHST- 2014/04/17 00:00 [accepted]
PHST- 2014/05/27 06:00 [entrez]
PHST- 2014/05/27 06:00 [pubmed]
PHST- 2015/01/07 06:00 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - ijms15057667 [pii]
AID - ijms-15-07667 [pii]
AID - 10.3390/ijms15057667 [doi]
PST - epublish
SO  - Int J Mol Sci. 2014 May 5;15(5):7667-83. doi: 10.3390/ijms15057667.