PMID- 24858028
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20140721
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 46
IP  - 2
DP  - 2014 Oct
TI  - A novel lineage transcription factor based analysis reveals differences in T 
      helper cell subpopulation development in infected and intrauterine growth 
      restricted (IUGR) piglets.
PG  - 333-40
LID - S0145-305X(14)00141-4 [pii]
LID - 10.1016/j.dci.2014.05.005 [doi]
AB  - Research in mouse and human clearly identified subsets of T helper (Th) cells 
      based on nuclear expression of specific lineage transcription factors. In swine, 
      however, transcription factor based detection of functional subpopulations of 
      porcine Th cells by flow cytometry is so far limited to regulatory T cells via 
      Foxp3. T-bet and GATA-3 are the transcription factors that regulate commitment to 
      Th1 or Th2 cells, respectively. In this study we prove GATA-3 and T-bet 
      expression in porcine CD4(+) cells polarized in vitro. Importantly, GATA-3 and 
      T-bet expressing cells were detectable in pigs infected with pathogens associated 
      with Th2 and Th1 immune responses. Increased frequencies of GATA-3 positive 
      CD4(+) cells are found in vivo in pigs experimentally infected with the nematode 
      Trichuris suis, whereas porcine reproductive and respiratory syndrome virus 
      (PRRSV) infection elicited T-bet positive CD4(+) T cells. Analysing the immune 
      status of pre-weaning piglets with intrauterine growth restriction (IUGR) we 
      found an increased expression of Foxp3, T-bet and GATA-3 in CD4(+) and 
      CD4(+)CD8(+) double-positive T cells in systemic and intestinal compartments of 
      IUGR piglets. Hence, we established the detection of porcine Th1 and Th2 cells 
      via T-bet and GATA-3 and show that the porcine lineage transcription factors are 
      differentially regulated very early in life depending on the developmental 
      status.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Ebner, F
AU  - Ebner F
AD  - Institute of Immunology, Department of Veterinary Medicine, Freie Universitat 
      Berlin, Robert-von-Ostertag-Strasse 7-13, 14163 Berlin, Germany.
FAU - Rausch, S
AU  - Rausch S
AD  - Institute of Immunology, Department of Veterinary Medicine, Freie Universitat 
      Berlin, Robert-von-Ostertag-Strasse 7-13, 14163 Berlin, Germany.
FAU - Scharek-Tedin, L
AU  - Scharek-Tedin L
AD  - Institute of Animal Nutrition, Department of Veterinary Medicine, Freie 
      Universitat Berlin, Konigin-Luise-Strasse 49, 14195 Berlin, Germany.
FAU - Pieper, R
AU  - Pieper R
AD  - Institute of Animal Nutrition, Department of Veterinary Medicine, Freie 
      Universitat Berlin, Konigin-Luise-Strasse 49, 14195 Berlin, Germany.
FAU - Burwinkel, M
AU  - Burwinkel M
AD  - Institute of Virology, Department of Veterinary Medicine, Freie Universitat 
      Berlin, Robert-von-Ostertag-Strasse 7-13, 14163 Berlin, Germany.
FAU - Zentek, J
AU  - Zentek J
AD  - Institute of Animal Nutrition, Department of Veterinary Medicine, Freie 
      Universitat Berlin, Konigin-Luise-Strasse 49, 14195 Berlin, Germany.
FAU - Hartmann, S
AU  - Hartmann S
AD  - Institute of Immunology, Department of Veterinary Medicine, Freie Universitat 
      Berlin, Robert-von-Ostertag-Strasse 7-13, 14163 Berlin, Germany. Electronic 
      address: Susanne.Hartmann@fu-berlin.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140521
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
SB  - IM
MH  - Animals
MH  - Birth Weight
MH  - Cell Lineage
MH  - Cell Polarity
MH  - Cells, Cultured
MH  - Fetal Growth Retardation/immunology/parasitology/*veterinary
MH  - GATA3 Transcription Factor/*metabolism
MH  - Sus scrofa
MH  - Swine
MH  - Swine Diseases/*immunology/parasitology
MH  - T-Box Domain Proteins/*metabolism
MH  - Th1 Cells/*immunology
MH  - Th2 Cells/*immunology
MH  - Trichuriasis/immunology/*veterinary
MH  - Trichuris/immunology
OTO - NOTNLM
OT  - GATA-3
OT  - Intrauterine growth restriction
OT  - Lineage commitment
OT  - Swine immunology
OT  - T-bet
OT  - Transcription factor
EDAT- 2014/05/27 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/05/27 06:00
PHST- 2014/02/04 00:00 [received]
PHST- 2014/05/07 00:00 [revised]
PHST- 2014/05/07 00:00 [accepted]
PHST- 2014/05/27 06:00 [entrez]
PHST- 2014/05/27 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - S0145-305X(14)00141-4 [pii]
AID - 10.1016/j.dci.2014.05.005 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2014 Oct;46(2):333-40. doi: 10.1016/j.dci.2014.05.005. Epub 
      2014 May 21.