PMID- 24858687
OWN - NLM
STAT- MEDLINE
DCOM- 20140921
LR  - 20211108
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 450
IP  - 1
DP  - 2014 Jul 18
TI  - The Fe-heme structure of met-indoleamine 2,3-dioxygenase-2 determined by X-ray 
      absorption fine structure.
PG  - 25-9
LID - S0006-291X(14)00926-7 [pii]
LID - 10.1016/j.bbrc.2014.05.054 [doi]
AB  - Multiple-scattering (MS) analysis of EXAFS data on met-indoleamine 
      2,3-dioxygenase-2 (IDO2) and analysis of XANES have provided the first direct 
      structural information about the axial donor ligands of the iron center for this 
      recently discovered protein. At 10K, it exists in a low-spin bis(His) form with 
      Fe-Np(av)=1.97A, the Fe-NIm bond lengths of 2.11A and 2.05A, which is in 
      equilibrium with a high-spin form at room temperature. The bond distances in the 
      low-spin form are consistent with other low-spin hemeproteins, as is the XANES 
      spectrum, which is closer to that of the low-spin met-Lb than that of the 
      high-spin met-Mb. The potential physiological role of this spin equilibrium is 
      discussed.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Aitken, Jade B
AU  - Aitken JB
AD  - School of Chemistry, The University of Sydney, NSW 2006, Australia; Australian 
      Synchrotron, Clayton, Victoria 3168, Australia; Institute of Materials Structure 
      Science, KEK, Tsukuba, Ibaraki 305-0801, Japan.
FAU - Austin, Christopher J D
AU  - Austin CJ
AD  - School of Chemistry, The University of Sydney, NSW 2006, Australia; Department of 
      Pathology and Bosch Institute, The University of Sydney, Camperdown, NSW 2006, 
      Australia.
FAU - Hunt, Nicholas H
AU  - Hunt NH
AD  - Department of Pathology and Bosch Institute, The University of Sydney, 
      Camperdown, NSW 2006, Australia.
FAU - Ball, Helen J
AU  - Ball HJ
AD  - Department of Pathology and Bosch Institute, The University of Sydney, 
      Camperdown, NSW 2006, Australia.
FAU - Lay, Peter A
AU  - Lay PA
AD  - School of Chemistry, The University of Sydney, NSW 2006, Australia. Electronic 
      address: peter.lay@sydney.edu.au.
LA  - eng
GR  - P41RR001209/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140522
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (IDO2 protein, human)
RN  - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
RN  - 42VZT0U6YR (Heme)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Computer Simulation
MH  - Heme/*chemistry
MH  - Indoleamine-Pyrrole 2,3,-Dioxygenase/*chemistry/*ultrastructure
MH  - Iron/*chemistry
MH  - *Models, Chemical
MH  - *Models, Molecular
MH  - Protein Conformation
OTO - NOTNLM
OT  - EXAFS
OT  - Heme environment
OT  - Indoleamine 2,3-dioxygenase-2 (IDO2)
OT  - Mixed-spin species
OT  - X-ray absorption fine structure
EDAT- 2014/05/27 06:00
MHDA- 2014/09/23 06:00
CRDT- 2014/05/27 06:00
PHST- 2014/05/07 00:00 [received]
PHST- 2014/05/14 00:00 [accepted]
PHST- 2014/05/27 06:00 [entrez]
PHST- 2014/05/27 06:00 [pubmed]
PHST- 2014/09/23 06:00 [medline]
AID - S0006-291X(14)00926-7 [pii]
AID - 10.1016/j.bbrc.2014.05.054 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2014 Jul 18;450(1):25-9. doi: 
      10.1016/j.bbrc.2014.05.054. Epub 2014 May 22.