PMID- 24859056
OWN - NLM
STAT- MEDLINE
DCOM- 20150409
LR  - 20140722
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 21
IP  - 2
DP  - 2014 Aug
TI  - Enhanced dendritic cell maturation by the B-chain of Korean mistletoe lectin 
      (KML-B), a novel TLR4 agonist.
PG  - 309-19
LID - S1567-5769(14)00182-9 [pii]
LID - 10.1016/j.intimp.2014.05.010 [doi]
AB  - Korean mistletoe lectin (KML) is composed of A and B sub-chains. The B-chain 
      binds to cell surfaces, whereas the A-chain hinders translation because it is a 
      RIP (ribosome inactivating protein) inducing apoptosis. Although KML has various 
      biological and immunological activities, its potential use in cancer therapy or 
      as an adjuvant therapy is limited by its toxicity to normal cells. This study was 
      conducted to determine whether the B-chain of KML (KML-B) has immunoadjuvant 
      activity and cytotoxicity activity. To evaluate the immunomodulatory activities 
      of B chain KML, in vitro experiments employing bone marrow-derived dendritic 
      cells (BMDCs) were performed. Dendritic cells (DCs) are a unique group of white 
      blood cells that are able to capture and process antigens for presentation to T 
      cells, which constitute primary immune response. In the present study, KML-B was 
      found to be non-cytotoxic to BMDCs. Furthermore, the expressions of 
      co-stimulatory molecules (CD40, CD80, CD86, and MHC II) and the secretions of 
      cytokines (IL-1beta, IL-6, IL-12p70, and TNF-alpha) were increased in BMDCs by KML-B. In 
      addition, other indicators (antigen-uptake and CCR7 expression) of BMDC 
      maturation were changed by KML-B, and the ability of KML-B to enhance various 
      functions by BMDCs was found to be dependent on TLR4 expression. Moreover, BMDCs 
      matured by KML-B induced naive CD4(+) T cell differentiation toward Th1 cells 
      directly and indirectly. These experiments confirm that KML-B exhibits potent 
      immunomodulatory properties and suggest that KML-B be considered a potential 
      dendritic cell-based cancer therapy and immunoadjuvant.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Kim, Jong-Jin
AU  - Kim JJ
AD  - Department of Biology, Sunchon National University, Suncheon, Republic of Korea.
FAU - Hwang, Yun-Ho
AU  - Hwang YH
AD  - Department of Pharmacy, Sunchon National University, Suncheon, Republic of Korea.
FAU - Kang, Kyung-Yun
AU  - Kang KY
AD  - Department of Pharmacy, Sunchon National University, Suncheon, Republic of Korea.
FAU - Kim, Inbo
AU  - Kim I
AD  - School of Life Science, Handong Global University, Pohang, Republic of Korea.
FAU - Kim, Jong-Bae
AU  - Kim JB
AD  - School of Life Science, Handong Global University, Pohang, Republic of Korea.
FAU - Park, Jong-Hwan
AU  - Park JH
AD  - Department of Biochemistry, College of Medicine, Konyang University, Daejeon, 
      Republic of Korea.
FAU - Yoo, Yung-Choon
AU  - Yoo YC
AD  - Department of Microbiology, College of Medicine, Konyang University, Daejeon, 
      Republic of Korea.
FAU - Yee, Sung-Tae
AU  - Yee ST
AD  - Department of Biology, Sunchon National University, Suncheon, Republic of Korea; 
      Department of Pharmacy, Sunchon National University, Suncheon, Republic of Korea. 
      Electronic address: sungtae@sunchon.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140521
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antigens, CD)
RN  - 0 (Interleukins)
RN  - 0 (Lectins)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Bone Marrow Cells/drug effects/metabolism
MH  - Cell Differentiation/drug effects
MH  - Dendritic Cells/*drug effects/metabolism
MH  - Female
MH  - Humans
MH  - Interleukins/metabolism
MH  - Lectins/*pharmacology
MH  - Lymphocyte Activation/*drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mistletoe/*chemistry
MH  - Th1 Cells/drug effects/metabolism
MH  - Toll-Like Receptor 4/*analysis
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - BMDCs (bone marrow-derived dendritic cells)
OT  - CD4(+) T cells
OT  - KML (Korean mistletoe lectin)
OT  - KML-B (B-chain of KML)
OT  - TLR4 (toll-liked receptor 4)
EDAT- 2014/05/27 06:00
MHDA- 2015/04/10 06:00
CRDT- 2014/05/27 06:00
PHST- 2014/03/20 00:00 [received]
PHST- 2014/04/24 00:00 [revised]
PHST- 2014/05/06 00:00 [accepted]
PHST- 2014/05/27 06:00 [entrez]
PHST- 2014/05/27 06:00 [pubmed]
PHST- 2015/04/10 06:00 [medline]
AID - S1567-5769(14)00182-9 [pii]
AID - 10.1016/j.intimp.2014.05.010 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2014 Aug;21(2):309-19. doi: 10.1016/j.intimp.2014.05.010. 
      Epub 2014 May 21.