PMID- 24862851
OWN - NLM
STAT- MEDLINE
DCOM- 20150302
LR  - 20200106
IS  - 1873-474X (Electronic)
IS  - 0736-5748 (Linking)
VI  - 36
DP  - 2014 Aug
TI  - Prenatal caffeine intake differently affects synaptic proteins during fetal brain 
      development.
PG  - 45-52
LID - S0736-5748(14)00071-9 [pii]
LID - 10.1016/j.ijdevneu.2014.04.006 [doi]
AB  - Caffeine is the psychostimulant most consumed worldwide. However, little is known 
      about its effects during fetal brain development. In this study, adult female 
      Wistar rats received caffeine in drinking water (0.1, 0.3 and 1.0 g/L) during the 
      active cycle in weekdays, two weeks before mating and throughout pregnancy. 
      Cerebral cortex and hippocampus from embryonic stages 18 or 20 (E18 or E20, 
      respectively) were collected for immunodetection of the following synaptic 
      proteins: brain-derived neurotrophic factor (BDNF), TrkB receptor, Sonic Hedgehog 
      (Shh), Growth Associated Protein 43 (GAP-43) and Synaptosomal-associated Protein 
      25 (SNAP-25). Besides, the estimation of NeuN-stained nuclei (mature neurons) and 
      non-neuronal nuclei was verified in both brain regions and embryonic periods. 
      Caffeine (1.0 g/L) decreased the body weight of embryos at E20. Cortical BDNF at 
      E18 was decreased by caffeine (1.0 g/L), while it increased at E20, with no major 
      effects on TrkB receptors. In the hippocampus, caffeine decreased TrkB receptor 
      only at E18, with no effects on BDNF. Moderate and high doses of caffeine 
      promoted an increase in Shh in both brain regions at E18, and in the hippocampus 
      at E20. Caffeine (0.3g/L) decreased GAP-43 only in the hippocampus at E18. The 
      NeuN-stained nuclei increased in the cortex at E20 by lower dose and in the 
      hippocampus at E18 by moderate dose. Our data revealed that caffeine transitorily 
      affect synaptic proteins during fetal brain development. The increased number of 
      NeuN-stained nuclei by prenatal caffeine suggests a possible acceleration of the 
      telencephalon maturation. Although some modifications in the synaptic proteins 
      were transient, our data suggest that caffeine even in lower doses may alter the 
      fetal brain development.
CI  - Copyright (c) 2014 ISDN. Published by Elsevier Ltd. All rights reserved.
FAU - Mioranzza, Sabrina
AU  - Mioranzza S
AD  - Laboratorio de Estudos sobre o Sistema Purinergico, Programa de Pos-Graduacao em 
      Ciencias Biologicas/Bioquimica, Porto Alegre, RS 90035-003, Brazil.
FAU - Nunes, Fernanda
AU  - Nunes F
AD  - Laboratorio de Estudos sobre o Sistema Purinergico, Programa de Pos-Graduacao em 
      Ciencias Biologicas/Bioquimica, Porto Alegre, RS 90035-003, Brazil.
FAU - Marques, Daniela M
AU  - Marques DM
AD  - Laboratorio de Estudos sobre o Sistema Purinergico, Programa de Pos-Graduacao em 
      Ciencias Biologicas/Bioquimica, Porto Alegre, RS 90035-003, Brazil.
FAU - Fioreze, Gabriela T
AU  - Fioreze GT
AD  - Laboratorio de Estudos sobre o Sistema Purinergico, Programa de Pos-Graduacao em 
      Ciencias Biologicas/Bioquimica, Porto Alegre, RS 90035-003, Brazil.
FAU - Rocha, Andreia S
AU  - Rocha AS
AD  - Laboratorio de Estudos sobre o Sistema Purinergico, Programa de Pos-Graduacao em 
      Ciencias Biologicas/Bioquimica, Porto Alegre, RS 90035-003, Brazil.
FAU - Botton, Paulo Henrique S
AU  - Botton PH
AD  - Laboratorio de Estudos sobre o Sistema Purinergico, Programa de Pos-Graduacao em 
      Ciencias Biologicas/Bioquimica, Porto Alegre, RS 90035-003, Brazil.
FAU - Costa, Marcelo S
AU  - Costa MS
AD  - Laboratorio de Estudos sobre o Sistema Purinergico, Programa de Pos-Graduacao em 
      Ciencias Biologicas/Bioquimica, Porto Alegre, RS 90035-003, Brazil.
FAU - Porciuncula, Lisiane O
AU  - Porciuncula LO
AD  - Laboratorio de Estudos sobre o Sistema Purinergico, Programa de Pos-Graduacao em 
      Ciencias Biologicas/Bioquimica, Porto Alegre, RS 90035-003, Brazil. Electronic 
      address: loporciuncula@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140524
PL  - United States
TA  - Int J Dev Neurosci
JT  - International journal of developmental neuroscience : the official journal of the 
      International Society for Developmental Neuroscience
JID - 8401784
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Central Nervous System Stimulants)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Shh protein, mouse)
RN  - 3G6A5W338E (Caffeine)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/*metabolism/pathology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Caffeine/metabolism/*toxicity
MH  - Central Nervous System Stimulants/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Embryo, Mammalian
MH  - Female
MH  - Fetal Development/*drug effects
MH  - Hedgehog Proteins/genetics/metabolism
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Neurons/metabolism/pathology
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects/chemically induced/pathology/physiopathology
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/drug effects
MH  - Synapses/drug effects/*metabolism
OTO - NOTNLM
OT  - BDNF
OT  - Brain development
OT  - Caffeine
OT  - Shh
OT  - Synaptic proteins
EDAT- 2014/05/28 06:00
MHDA- 2015/03/03 06:00
CRDT- 2014/05/28 06:00
PHST- 2014/03/12 00:00 [received]
PHST- 2014/04/23 00:00 [revised]
PHST- 2014/04/30 00:00 [accepted]
PHST- 2014/05/28 06:00 [entrez]
PHST- 2014/05/28 06:00 [pubmed]
PHST- 2015/03/03 06:00 [medline]
AID - S0736-5748(14)00071-9 [pii]
AID - 10.1016/j.ijdevneu.2014.04.006 [doi]
PST - ppublish
SO  - Int J Dev Neurosci. 2014 Aug;36:45-52. doi: 10.1016/j.ijdevneu.2014.04.006. Epub 
      2014 May 24.