PMID- 24863266 OWN - NLM STAT- MEDLINE DCOM- 20160407 LR - 20181202 IS - 1099-0496 (Electronic) IS - 1099-0496 (Linking) VI - 50 IP - 7 DP - 2015 Jul TI - Val66Met polymorphism in the BDNF gene in children with bronchial asthma. PG - 631-7 LID - 10.1002/ppul.23065 [doi] AB - OBJECTIVES: Bronchial asthma is a chronic respiratory disease characterized by airway inflammation. There is increasing evidence that neurotrophins play an important role in the development and maintenance of neurogenic airway inflammation in chronic allergic diseases. WORKING HYPOTHESIS: Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family and has several important functions in the airways. There are only a few reports on the association between genetic variations in the BDNF gene and various allergic diseases, and the results are generally conflicting. Therefore, we aimed to study the functional polymorphism Val66Met (also called rs6265 or G196A) in the BDNF gene in a group of asthmatic children and healthy controls. STUDY DESIGN, PATIENT-SELECTION, AND METHODOLOGY: We studied 248 asthmatic patients (aged 12.28 +/- 0.24 years) and 249 healthy children (aged 13.14 +/- 0.48 years). Analysis of the Val66Met polymorphism of the BDNF gene was performed by polymerase chain reaction (PCR) and PCR products were digested by PmlI. RESULTS: The prevalence of the Val66Met polymorphisms (Val/Val, Val/Met, and Met/Met) was 61.7%, 33.5%, and 4.8% in asthmatics, respectively, and 47.0%, 51.8%, and 1.2% in healthy subjects, respectively. We observed a significant association of the Met/Met variant genotype with asthmatics (OR = 4.17, 95% CI = 1.16-14.96, P = 0.018). The Val/Met genotype was protective against bronchial asthma (OR = 0.69, 95% CI = 0.48-0.99, P = 0.045), especially in girls (OR = 0.34, 95% CI = 0.20-0.59, P = 0.001). CONCLUSION: Specific BDNF gene polymorphism may contribute to bronchial asthma susceptibility. Our study suggested the positive association between selected functional BDNF polymorphism (rs6265) and asthma in children. CI - (c) 2014 Wiley Periodicals, Inc. FAU - Jesenak, Milos AU - Jesenak M AD - Department of Paediatrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia. FAU - Babusikova, Eva AU - Babusikova E AD - Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia. FAU - Evinova, Andrea AU - Evinova A AD - Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia. FAU - Banovcin, Peter AU - Banovcin P AD - Department of Paediatrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia. FAU - Dobrota, Dusan AU - Dobrota D AD - Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140526 PL - United States TA - Pediatr Pulmonol JT - Pediatric pulmonology JID - 8510590 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 7171WSG8A2 (BDNF protein, human) SB - IM MH - Adolescent MH - Asthma/*genetics MH - Brain-Derived Neurotrophic Factor/*genetics MH - Case-Control Studies MH - Child MH - Child, Preschool MH - Female MH - Genetic Predisposition to Disease MH - Genotype MH - Humans MH - Male MH - *Polymorphism, Genetic OTO - NOTNLM OT - brain-derived neurotrophic factor OT - bronchial asthma OT - children OT - gene polymorphism OT - neurogenic inflammation OT - neurotrophins EDAT- 2014/05/28 06:00 MHDA- 2016/04/08 06:00 CRDT- 2014/05/28 06:00 PHST- 2013/05/06 00:00 [received] PHST- 2014/04/21 00:00 [accepted] PHST- 2014/05/28 06:00 [entrez] PHST- 2014/05/28 06:00 [pubmed] PHST- 2016/04/08 06:00 [medline] AID - 10.1002/ppul.23065 [doi] PST - ppublish SO - Pediatr Pulmonol. 2015 Jul;50(7):631-7. doi: 10.1002/ppul.23065. Epub 2014 May 26.