PMID- 24863696
OWN - NLM
STAT- MEDLINE
DCOM- 20150818
LR  - 20220321
IS  - 1573-7217 (Electronic)
IS  - 0167-6806 (Print)
IS  - 0167-6806 (Linking)
VI  - 146
IP  - 1
DP  - 2014 Jul
TI  - The metastatic potential of triple-negative breast cancer is decreased via 
      caloric restriction-mediated reduction of the miR-17~92 cluster.
PG  - 41-50
LID - 10.1007/s10549-014-2978-7 [doi]
AB  - Caloric restriction (CR) has been shown to cause tumor regression in models of 
      triple-negative breast cancer (TNBC), and the regression is augmented when 
      coupled with ionizing radiation (IR). In this study, we sought to determine if 
      the molecular interaction between CR and IR could be mediated by microRNA (miR). 
      miR arrays revealed 3 miRs in the miR-17~92 cluster as most significantly down 
      regulated when CR is combined with IR. In vivo, CR and IR down regulated 
      miR-17/20 in 2 TNBC models. To elucidate the mechanism by which this cluster 
      regulates the response to CR, cDNA arrays were performed and the top 5 
      statistically significant gene ontology terms with high fold changes were all 
      associated with extracellular matrix (ECM) and metastases. In silico analysis 
      revealed 4 potential targets of the miR-17~92 cluster related to ECM: collagen 4 
      alpha 3, laminin alpha 3, and metallopeptidase inhibitors 2 and 3, which were 
      confirmed by luciferase assays. The overexpression or silencing of miR-17/20a 
      demonstrated that those miRs directly affected the ECM proteins. Furthermore, we 
      found that CR-mediated inhibition of miR-17/20a can regulate the expression of 
      ECM proteins. Functionally, we demonstrate that CR decreases the metastatic 
      potential of cells which further demonstrates the importance of the ECM. In 
      conclusion, CR can be used as a potential treatment for cancer because it may 
      alter many molecular targets concurrently and decrease metastatic potential for 
      TNBC.
FAU - Jin, Lianjin
AU  - Jin L
AD  - Department of Radiation Oncology, Thomas Jefferson University, Phiadelphia, PA, 
      19107, USA.
FAU - Lim, Meng
AU  - Lim M
FAU - Zhao, Shuping
AU  - Zhao S
FAU - Sano, Yuri
AU  - Sano Y
FAU - Simone, Brittany A
AU  - Simone BA
FAU - Savage, Jason E
AU  - Savage JE
FAU - Wickstrom, Eric
AU  - Wickstrom E
FAU - Camphausen, Kevin
AU  - Camphausen K
FAU - Pestell, Richard G
AU  - Pestell RG
FAU - Simone, Nicole L
AU  - Simone NL
LA  - eng
GR  - P30 CA056036/CA/NCI NIH HHS/United States
GR  - ZIA BC010873-03/Intramural NIH HHS/United States
GR  - P30-CA56036/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140527
PL  - Netherlands
TA  - Breast Cancer Res Treat
JT  - Breast cancer research and treatment
JID - 8111104
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-3)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Caloric Restriction
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Disease Models, Animal
MH  - Extracellular Matrix Proteins/chemistry/genetics/metabolism
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Mice
MH  - MicroRNAs/chemistry/*genetics
MH  - *Multigene Family
MH  - Neoplasm Metastasis
MH  - RNA, Messenger/chemistry/genetics
MH  - Radiation, Ionizing
MH  - Sequence Alignment
MH  - Tissue Inhibitor of Metalloproteinase-2/genetics/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-3/genetics/metabolism
MH  - Triple Negative Breast Neoplasms/*genetics/metabolism/*pathology
PMC - PMC4157915
MID - NIHMS613371
COIS- Conflict of interest The authors declare that they have no conflict of interest.
EDAT- 2014/05/28 06:00
MHDA- 2015/08/19 06:00
PMCR- 2015/07/01
CRDT- 2014/05/28 06:00
PHST- 2014/02/01 00:00 [received]
PHST- 2014/04/19 00:00 [accepted]
PHST- 2014/05/28 06:00 [entrez]
PHST- 2014/05/28 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - 10.1007/s10549-014-2978-7 [doi]
PST - ppublish
SO  - Breast Cancer Res Treat. 2014 Jul;146(1):41-50. doi: 10.1007/s10549-014-2978-7. 
      Epub 2014 May 27.