PMID- 24867354
OWN - NLM
STAT- MEDLINE
DCOM- 20160308
LR  - 20221207
IS  - 1369-1635 (Electronic)
IS  - 0953-7104 (Linking)
VI  - 26
IP  - 5
DP  - 2015
TI  - Platelet-derived RANK ligand enhances CCL17 secretion from dendritic cells 
      mediated by thymic stromal lymphopoietin.
PG  - 425-31
LID - 10.3109/09537104.2014.920081 [doi]
AB  - Dendritic cells (DCs) play an integral role in cellular cascade that initiate and 
      maintain Th2 responses in allergy. In this study, we examined the interaction 
      between platelets and DCs to determine the role of platelets in the intervention 
      of immune responses through modulation of DC functions. Blood-purified myeloid 
      DCs, which had been stimulated with thymic stromal lymphopoietin (TSLP-DCs), 
      formed aggregates with activated platelets. TSLP-DC maturation was induced after 
      the interaction with TRAP6-activated platelets as indicated by an increase in the 
      expression of CD86, CD40, and CD83. In addition, production of a Th2 
      cell-attracting chemokine, CCL17, was clearly upregulated by coculture of 
      TSLP-DCs with TRAP6-activated platelets. We further found that an expression of 
      RANK ligand (RANKL) on platelets was upregulated by the TRAP6 activation, and 
      that, using the neutralizing antibody against RANKL, the platelet-derived RANKL 
      induces the activation of TSLP-DCs. Thus, activated platelets can intervene in 
      adaptive immune responses through induction of functional modulation of TSLP-DCs. 
      Platelets have the ability to enhance the DC-mediated Th2 response and may 
      contribute to the allergic inflammation. In conclusion, our study provides new 
      insights in platelet functions and the possible mechanism of allergic responses 
      that stem from DCs.
FAU - Nakanishi, Takahisa
AU  - Nakanishi T
AD  - First Department of Internal Medicine, Kansai Medical University , Osaka , Japan.
FAU - Inaba, Muneo
AU  - Inaba M
FAU - Inagaki-Katashiba, Noriko
AU  - Inagaki-Katashiba N
FAU - Tanaka, Akihiro
AU  - Tanaka A
FAU - Vien, Phan Thi Xuan
AU  - Vien PT
FAU - Kibata, Kayoko
AU  - Kibata K
FAU - Ito, Tomoki
AU  - Ito T
FAU - Nomura, Shosaku
AU  - Nomura S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140527
PL  - England
TA  - Platelets
JT  - Platelets
JID - 9208117
RN  - 0 (Antigens, Surface)
RN  - 0 (Chemokine CCL17)
RN  - 0 (Cytokines)
RN  - 0 (RANK Ligand)
RN  - 0 (Receptor Activator of Nuclear Factor-kappa B)
RN  - GT0IL38SP4 (Thymic Stromal Lymphopoietin)
SB  - IM
MH  - Adult
MH  - Antigens, Surface/metabolism
MH  - Blood Platelets/*metabolism
MH  - Cell Adhesion
MH  - Chemokine CCL17/*metabolism
MH  - Cytokines/*metabolism
MH  - Dendritic Cells/drug effects/*immunology/*metabolism
MH  - Female
MH  - Healthy Volunteers
MH  - Humans
MH  - Immunophenotyping
MH  - Male
MH  - Middle Aged
MH  - Platelet Activation
MH  - Platelet Aggregation
MH  - RANK Ligand/*metabolism/pharmacology
MH  - Receptor Activator of Nuclear Factor-kappa B/metabolism
MH  - Th2 Cells/immunology/metabolism
MH  - Thymic Stromal Lymphopoietin
OTO - NOTNLM
OT  - CCL17
OT  - RANK ligand
OT  - dendritic cells
OT  - platelets
OT  - thymic stromal lymphopoietin
EDAT- 2014/05/29 06:00
MHDA- 2016/03/10 06:00
CRDT- 2014/05/29 06:00
PHST- 2014/05/29 06:00 [entrez]
PHST- 2014/05/29 06:00 [pubmed]
PHST- 2016/03/10 06:00 [medline]
AID - 10.3109/09537104.2014.920081 [doi]
PST - ppublish
SO  - Platelets. 2015;26(5):425-31. doi: 10.3109/09537104.2014.920081. Epub 2014 May 
      27.