PMID- 24867422
OWN - NLM
STAT- MEDLINE
DCOM- 20150625
LR  - 20211021
IS  - 1573-904X (Electronic)
IS  - 0724-8741 (Linking)
VI  - 31
IP  - 11
DP  - 2014 Nov
TI  - Modulating protein release profiles by incorporating hyaluronic acid into PLGA 
      microparticles Via a spray dryer equipped with a 3-fluid nozzle.
PG  - 2940-51
LID - 10.1007/s11095-014-1387-2 [doi]
AB  - PURPOSE: The purpose of this study was to modulate the release profiles of the 
      model protein drug from spray dried poly(DL-lactic-co-glycolic acid) (PLGA) 
      microparticles by incorporating hyaluronic acid (HA) in the formulation. METHODS: 
      Bovine serum albumin (BSA)-loaded PLGA microparticles with or without HA were 
      prepared using a spray dryer equipped with a 3-fluid nozzle. The effects of HA on 
      the surface tension and the rheological behavior of the inner feed solution were 
      investigated. The physicochemical properties of the resulting microparticles were 
      characterized using scanning electron microscopy (SEM), laser diffraction (LD), 
      confocal laser scanning microscopy (CLSM) and X-ray photoelectron spectroscopy 
      (XPS). Circular dischoism (CD) was used to characterize conformational integrity 
      of BSA released from the microparticles. RESULTS: Spherical microparticles with 
      D50 of 5-10 mum were obtained. Addition of HA in inner feed solutions increased 
      the feed viscosity, but with no influence on the surface tension. All inner feed 
      solutions showed non-Newtonian shear thinning behavior and the rheological 
      properties were not time dependent. The CLSM and XPS analyses suggested a 
      core-shell like structure of the microparticles when HA was incorporated. The 
      release profiles of BSA were extended and the initial burst releases were 
      suppressed with an increase in HA in the microparticle formulations. In addition, 
      HA seemed to protect BSA from degradation upon the spray-drying process. 
      CONCLUSIONS: The present work demonstrates the potential of HA to modulate 
      protein release profile from PLGA microparticle formulations produced via spray 
      drying using 3-fluid nozzle.
FAU - Wan, Feng
AU  - Wan F
AD  - Department of Pharmacy, Faculty of Health and Medical Sciences, University of 
      Copenhagen, Universitetsparken 2, 2100, Copenhagen O, Denmark.
FAU - Maltesen, Morten Jonas
AU  - Maltesen MJ
FAU - Andersen, Sune Klint
AU  - Andersen SK
FAU - Bjerregaard, Simon
AU  - Bjerregaard S
FAU - Baldursdottir, Stefania G
AU  - Baldursdottir SG
FAU - Foged, Camilla
AU  - Foged C
FAU - Rantanen, Jukka
AU  - Rantanen J
FAU - Yang, Mingshi
AU  - Yang M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140528
PL  - United States
TA  - Pharm Res
JT  - Pharmaceutical research
JID - 8406521
RN  - 0 (Drug Carriers)
RN  - 0 (Proteins)
RN  - 0 (Solutions)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Chemistry, Pharmaceutical/methods
MH  - Drug Carriers/chemistry
MH  - Drug Compounding/*methods
MH  - Hyaluronic Acid/*chemistry
MH  - Lactic Acid/*chemistry
MH  - Microspheres
MH  - Particle Size
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Proteins/*chemistry
MH  - Rheology
MH  - Serum Albumin, Bovine/chemistry
MH  - Solutions/chemistry
MH  - Surface Tension
EDAT- 2014/05/29 06:00
MHDA- 2015/06/26 06:00
CRDT- 2014/05/29 06:00
PHST- 2014/01/17 00:00 [received]
PHST- 2014/04/07 00:00 [accepted]
PHST- 2014/05/29 06:00 [entrez]
PHST- 2014/05/29 06:00 [pubmed]
PHST- 2015/06/26 06:00 [medline]
AID - 10.1007/s11095-014-1387-2 [doi]
PST - ppublish
SO  - Pharm Res. 2014 Nov;31(11):2940-51. doi: 10.1007/s11095-014-1387-2. Epub 2014 May 
      28.