PMID- 24867429
OWN - NLM
STAT- MEDLINE
DCOM- 20150417
LR  - 20240326
IS  - 1879-1123 (Electronic)
IS  - 1044-0305 (Print)
IS  - 1044-0305 (Linking)
VI  - 25
IP  - 7
DP  - 2014 Jul
TI  - NanoLC/ESI+ HRMS3 quantitation of DNA adducts induced by 1,3-butadiene.
PG  - 1124-35
LID - 10.1007/s13361-014-0916-x [doi]
AB  - Human exposure to 1,3-butadiene (BD) present in automobile exhaust, cigarette 
      smoke, and forest fires is of great concern because of its potent 
      carcinogenicity. The adverse health effects of BD are mediated by its epoxide 
      metabolites such as 3,4-epoxy-1-butene (EB), which covalently modify genomic DNA 
      to form promutagenic nucleobase adducts. Because of their direct role in cancer, 
      BD-DNA adducts can be used as mechanism-based biomarkers of BD exposure. In the 
      present work, a mass spectrometry-based methodology was developed for accurate, 
      sensitive, and precise quantification of EB-induced N-7-(1-hydroxy-3-buten-2-yl) 
      guanine (EB-GII) DNA adducts in vivo. In our approach, EB-GII adducts are 
      selectively released from DNA backbone by neutral thermal hydrolysis, followed by 
      ultrafiltration, offline HPLC purification, and isotope dilution 
      nanoLC/ESI(+)-HRMS(3) analysis on an Orbitrap Velos mass spectrometer. Following 
      method validation, EB-GII lesions were quantified in human fibrosarcoma (HT1080) 
      cells treated with micromolar concentrations of EB and in liver tissues of rats 
      exposed to sub-ppm concentrations of BD (0.5-1.5 ppm). EB-GII concentrations 
      increased linearly from 1.15 +/- 0.23 to 10.11 +/- 0.45 adducts per 10(8) nucleotides 
      in HT1080 cells treated with 0.5-10 muM EB. EB-GII concentrations in DNA of 
      laboratory rats exposed to 0.5, 1.0, and 1.5 ppm BD were 0.17 +/- 0.05, 
      0.33 +/- 0.08, and 0.50 +/- 0.04 adducts per 10(8) nucleotides, respectively 
      [corrected]. We also used the new method to determine the in vivo half-life of 
      EB-GII adducts in rat liver DNA (2.20 +/- 0.12 d) and to detect EB-GII in human 
      blood DNA. To our knowledge, this is the first application of 
      nanoLC/ESI(+)-HRMS(3) Orbitrap methodology to quantitative analysis of DNA 
      adducts in vivo.
FAU - Sangaraju, Dewakar
AU  - Sangaraju D
AD  - Department of Medicinal Chemistry and the Masonic Cancer Center, University of 
      Minnesota, Minneapolis, MN, 55455, USA.
FAU - Villalta, Peter W
AU  - Villalta PW
FAU - Wickramaratne, Susith
AU  - Wickramaratne S
FAU - Swenberg, James
AU  - Swenberg J
FAU - Tretyakova, Natalia
AU  - Tretyakova N
LA  - eng
GR  - CA-77598/CA/NCI NIH HHS/United States
GR  - P30 CA077598/CA/NCI NIH HHS/United States
GR  - S10 RR024618/RR/NCRR NIH HHS/United States
GR  - 1P01 CA138338/CA/NCI NIH HHS/United States
GR  - HHSN261200644002/PHS HHS/United States
GR  - N01 CN064402/CN/NCI NIH HHS/United States
GR  - S10-RR-024618/RR/NCRR NIH HHS/United States
GR  - P01 CA138338/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140528
PL  - United States
TA  - J Am Soc Mass Spectrom
JT  - Journal of the American Society for Mass Spectrometry
JID - 9010412
RN  - 0 (Butadienes)
RN  - 0 (DNA Adducts)
RN  - 0 (Epoxy Compounds)
RN  - 478ERR5NKR (3,4-epoxy-1-butene)
RN  - JSD5FGP5VD (1,3-butadiene)
SB  - IM
EIN - J Am Soc Mass Spectrom. 2014 Sep;25(9):1674
MH  - Adult
MH  - Animals
MH  - Butadienes/*chemistry/metabolism
MH  - Cell Line, Tumor
MH  - DNA Adducts/*analysis/*chemistry/metabolism
MH  - Environmental Exposure/*analysis
MH  - Epoxy Compounds/analysis/chemistry/metabolism
MH  - Female
MH  - Humans
MH  - Liver/chemistry/drug effects
MH  - Male
MH  - Middle Aged
MH  - Rats
MH  - Rats, Inbred F344
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Young Adult
PMC - PMC5076477
MID - NIHMS822244
EDAT- 2014/05/29 06:00
MHDA- 2015/04/18 06:00
PMCR- 2016/10/24
CRDT- 2014/05/29 06:00
PHST- 2014/01/16 00:00 [received]
PHST- 2014/04/14 00:00 [accepted]
PHST- 2014/04/11 00:00 [revised]
PHST- 2014/05/29 06:00 [entrez]
PHST- 2014/05/29 06:00 [pubmed]
PHST- 2015/04/18 06:00 [medline]
PHST- 2016/10/24 00:00 [pmc-release]
AID - 10.1007/s13361-014-0916-x [doi]
PST - ppublish
SO  - J Am Soc Mass Spectrom. 2014 Jul;25(7):1124-35. doi: 10.1007/s13361-014-0916-x. 
      Epub 2014 May 28.