PMID- 24867433
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20140721
IS  - 1600-079X (Electronic)
IS  - 0742-3098 (Linking)
VI  - 57
IP  - 1
DP  - 2014 Aug
TI  - Melatonin improves mitochondrial function in inguinal white adipose tissue of 
      Zucker diabetic fatty rats.
PG  - 103-9
LID - 10.1111/jpi.12147 [doi]
AB  - Mitochondrial dysfunction in adipose tissue may contribute to obesity-related 
      metabolic derangements such as type 2 diabetes mellitus (T2DM). Because 
      mitochondria are a target for melatonin action, the goal of this study was to 
      investigate the effects of melatonin on mitochondrial function in white (WAT) and 
      beige inguinal adipose tissue of Zucker diabetic fatty (ZDF) rats, a model of 
      obesity-related T2DM. In this experimental model, melatonin reduces obesity and 
      improves the metabolic profile. At 6 wk of age, ZDF rats and lean littermates 
      (ZL) were subdivided into two groups, each composed of four rats: control (C-ZDF 
      and C-ZL) and treated with oral melatonin in the drinking water (10 mg/kg/day) 
      for 6 wk (M-ZDF and M-ZL). After the treatment period, animals were sacrificed, 
      tissues dissected, and mitochondrial function assessed in isolated organelles. 
      Melatonin increased the respiratory control ratio (RCR) in mitochondria from 
      white fat of both lean (by 26.5%, P < 0.01) and obese (by 34.5%, P < 0.01) rats 
      mainly through a reduction of proton leaking component of respiration (state 4) 
      (28% decrease in ZL, P < 0.01 and 35% in ZDF, P < 0.01). However, melatonin 
      treatment lowered the RCR in beige mitochondria of both lean (by 7%, P < 0.05) 
      and obese (by 13%, P < 0.05) rats by maintaining high rates of uncoupled 
      respiration. Melatonin also lowered mitochondrial oxidative status by reducing 
      nitrite levels and by increasing superoxide dismutase activity. Moreover, 
      melatonin treatment also caused a profound inhibition of Ca-induced opening of 
      mPTP in isolated mitochondria from both types of fat, white and beige, in both 
      lean and obese rats. These results demonstrate that chronic oral melatonin 
      improves mitochondrial respiration and reduces the oxidative status and 
      susceptibility to apoptosis in white and beige adipocytes. These melatonin 
      effects help to prevent mitochondrial dysfunction and thereby to improve 
      obesity-related metabolic disorders such as diabetes and dyslipidemia of ZDF 
      rats.
CI  - (c) 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Jimenez-Aranda, Aroa
AU  - Jimenez-Aranda A
AD  - Department of Pharmacology and Neurosciences Institute, School of Medicine, 
      University of Granada, Granada, Spain.
FAU - Fernandez-Vazquez, Gumersindo
AU  - Fernandez-Vazquez G
FAU - Mohammad A-Serrano, Maria
AU  - Mohammad A-Serrano M
FAU - Reiter, Russel J
AU  - Reiter RJ
FAU - Agil, Ahmad
AU  - Agil A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140710
PL  - England
TA  - J Pineal Res
JT  - Journal of pineal research
JID - 8504412
RN  - JL5DK93RCL (Melatonin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adipose Tissue, White/*drug effects/*metabolism
MH  - Animals
MH  - Calcium/pharmacology
MH  - Cell Respiration/drug effects
MH  - Male
MH  - Melatonin/*pharmacology
MH  - Mitochondria/*drug effects
MH  - Rats
MH  - Rats, Zucker
OTO - NOTNLM
OT  - Zucker diabetic fatty rats
OT  - beige adipose tissue
OT  - melatonin
OT  - mitochondrial function
OT  - white adipose tissue
EDAT- 2014/05/29 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/05/29 06:00
PHST- 2014/04/08 00:00 [received]
PHST- 2014/05/23 00:00 [accepted]
PHST- 2014/05/29 06:00 [entrez]
PHST- 2014/05/29 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - 10.1111/jpi.12147 [doi]
PST - ppublish
SO  - J Pineal Res. 2014 Aug;57(1):103-9. doi: 10.1111/jpi.12147. Epub 2014 Jul 10.