PMID- 24867521
OWN - NLM
STAT- MEDLINE
DCOM- 20150406
LR  - 20181202
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 18
IP  - 9
DP  - 2014
TI  - Epimorphin-induced differentiation of human umbilical cord mesenchymal stem cells 
      into sweat gland cells.
PG  - 1404-10
LID - 7366 [pii]
AB  - OBJECTIVES: Mesenchymal stem cells (MSCs) have the potential for 
      multi-directional differentiation and can be induced to differentiate into sweat 
      gland cells under certain conditions. Epimorphin (EPM) plays an important role in 
      the promotion of epithelial cell morphogenesis; however, its effect on sweat 
      gland-cell differentiation of MSCs remains unknown. The purpose of this study was 
      to investigate how EPM regulates sweat gland cell differentiation of human 
      umbilical cord mesenchymal stem cells (hUCMSCs). MATERIALS AND METHODS: hUCMSCs 
      were labeled with 5-bromo-2-deoxyuridine (BrdU) before differentiation induction; 
      were cultured in common culture medium, conditioned medium, or EPM-conditioned 
      medium; and then induced to differentiate into sweat gland cells. Five days after 
      induction, the expression rates of the sweat gland-cell antigens cytokeratin 14 
      (CK14), cytokeratin 19 (CK19), and carcinoembryonic antigen (CEA) in hUCMSCs were 
      detected by flow cytometry, and the messenger ribonucleic acid (mRNA) and protein 
      levels of CK14, CK19, and CEA were determined by reverse transcription polymerase 
      chain reaction (RT-PCR) and western blot, respectively. RESULTS: hUCMSCs can be 
      induced to differentiate into sweat gland cells in conditioned medium, and 
      expression of CEA was detected by immunofluorescence assay. Flow cytometry 
      results showed that the expression rate of the sweat gland-cell antigens CK14, 
      CK19, and CEA in the conditioned medium were significantly lower than that in the 
      EPM conditioned medium (p < 0.05). RT-PCR and western blot results showed that 
      the mRNA and protein levels of CK14, CK19, and CEA in the conditioned medium were 
      all significantly lower than that in the EPM-conditioned medium (p < 0.01). 
      CONCLUSIONS: These results suggest that EPM can effectively induce the 
      differentiation of hUCMSCs into sweat gland cells.
FAU - Tao, R
AU  - Tao R
AD  - Department of Plastic Surgery, PLA General Hospital, Beijing, People's Republic 
      of China. dochyf@163.com.
FAU - Sun, T-j
AU  - Sun TJ
FAU - Han, Y-q
AU  - Han YQ
FAU - Xu, G
AU  - Xu G
FAU - Liu, J
AU  - Liu J
FAU - Han, Y-f
AU  - Han YF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Biomarkers)
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (KRT14 protein, human)
RN  - 0 (Keratin-14)
RN  - 0 (Keratin-19)
RN  - 0 (Qa-SNARE Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Carcinoembryonic Antigen/genetics/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Shape
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Keratin-14/genetics/metabolism
MH  - Keratin-19/genetics/metabolism
MH  - Mesenchymal Stem Cells/*drug effects/metabolism
MH  - Phenotype
MH  - Qa-SNARE Proteins/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Sweat Glands/cytology/*drug effects/metabolism
MH  - Time Factors
MH  - Umbilical Cord/cytology/*drug effects/metabolism
EDAT- 2014/05/29 06:00
MHDA- 2015/04/07 06:00
CRDT- 2014/05/29 06:00
PHST- 2014/05/29 06:00 [entrez]
PHST- 2014/05/29 06:00 [pubmed]
PHST- 2015/04/07 06:00 [medline]
AID - 7366 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2014;18(9):1404-10.