PMID- 24867614
OWN - NLM
STAT- MEDLINE
DCOM- 20150415
LR  - 20140818
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 189
DP  - 2014 Aug 30
TI  - Infectious hypodermal and hematopoietic necrosis virus from Brazil: Sequencing, 
      comparative analysis and PCR detection.
PG  - 136-46
LID - S0168-1702(14)00212-3 [pii]
LID - 10.1016/j.virusres.2014.05.008 [doi]
AB  - A 3739 nucleotide fragment of Infectious hypodermal and hematopoietic necrosis 
      virus (IHHNV) from Brazil was amplified and sequenced. This fragment contains the 
      entire coding sequences of viral proteins, the full 3' untranslated region 
      (3'UTR) and a partial sequence of 5' untranslated region (5'UTR). The genome 
      organization of IHHNV revealed the three typical major coding domains: a left 
      ORF1 of 2001 bp that codes NS1, a left ORF2 (NS2) of 1091 bp that codes NS2 and a 
      right ORF3 of 990 bp that codes VP. Nucleotide and amino acid sequences of the 
      three viral proteins were compared with putative amino acid sequences of viruses 
      reported from different regions. Comparisons among genomes from different 
      geographic locations reveal 31 nucleotide regions that are 100% similar, 
      distributed throughout the genome. An analysis of secondary structure of UTR 
      regions, revealed regions with high probability to form hairpins, that may be 
      involved in mechanisms of viral replication. Additionally, a maximum likelihood 
      analysis indicates that Brazilian IHHNV belongs to lineage III, in the infectious 
      IHHNV group, and is clustered with IHHNV isolates from Hawaii, China, Taiwan, 
      Vietnam and South Korea. A new nested PCR targeting conserved nucleotide regions 
      is proposed to detect IHHNV.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Silva, Douglas C D
AU  - Silva DC
AD  - Laboratorio de Biologia Molecular Aplicada - LAPLIC, Departamento de Bioquimica, 
      Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
FAU - Nunes, Allan R D
AU  - Nunes AR
AD  - Laboratorio de Biologia Molecular Aplicada - LAPLIC, Departamento de Bioquimica, 
      Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
FAU - Teixeira, Darlio I A
AU  - Teixeira DI
AD  - Escola Agricola de Jundiai, Universidade Federal do Rio Grande do Norte, Brazil.
FAU - Lima, Joao Paulo M S
AU  - Lima JP
AD  - Laboratorio de Glicobiologia Molecular, Departamento de Bioquimica, Universidade 
      Federal do Rio Grande do Norte, Natal, Brazil; Programa de Pos-Graduacao em 
      Bioquimica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
FAU - Lanza, Daniel C F
AU  - Lanza DC
AD  - Laboratorio de Biologia Molecular Aplicada - LAPLIC, Departamento de Bioquimica, 
      Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil. Electronic 
      address: danielclanza@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140524
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - 3' Untranslated Regions
MH  - 5' Untranslated Regions
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brazil
MH  - Cluster Analysis
MH  - DNA, Viral/*chemistry/*genetics
MH  - Densovirinae/*classification/genetics/*isolation & purification
MH  - Gene Order
MH  - *Genome, Viral
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Phylogeny
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
MH  - Viral Proteins/genetics
OTO - NOTNLM
OT  - Brevidensovirus
OT  - Penaeus stitlirostris densovirus
OT  - Shrimp nanism
OT  - White Spot Syndrome
EDAT- 2014/05/29 06:00
MHDA- 2015/04/16 06:00
CRDT- 2014/05/29 06:00
PHST- 2014/01/15 00:00 [received]
PHST- 2014/05/07 00:00 [revised]
PHST- 2014/05/11 00:00 [accepted]
PHST- 2014/05/29 06:00 [entrez]
PHST- 2014/05/29 06:00 [pubmed]
PHST- 2015/04/16 06:00 [medline]
AID - S0168-1702(14)00212-3 [pii]
AID - 10.1016/j.virusres.2014.05.008 [doi]
PST - ppublish
SO  - Virus Res. 2014 Aug 30;189:136-46. doi: 10.1016/j.virusres.2014.05.008. Epub 2014 
      May 24.