PMID- 24869935
OWN - NLM
STAT- MEDLINE
DCOM- 20141118
LR  - 20240321
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 124
IP  - 4
DP  - 2014 Jul 24
TI  - Muscleblind-like 1 (Mbnl1) regulates pre-mRNA alternative splicing during 
      terminal erythropoiesis.
PG  - 598-610
LID - 10.1182/blood-2013-12-542209 [doi]
AB  - The scope and roles of regulated isoform gene expression during erythroid 
      terminal development are poorly understood. We identified hundreds of 
      differentiation-associated isoform changes during terminal erythropoiesis. 
      Sequences surrounding cassette exons of skipped exon events are enriched for 
      motifs bound by the Muscleblind-like (MBNL) family of splicing factors. Knockdown 
      of Mbnl1 in cultured murine fetal liver erythroid progenitors resulted in a 
      strong block in erythroid differentiation and disrupted the developmentally 
      regulated exon skipping of Ndel1 mRNA, which is bound by MBNL1 and critical for 
      erythroid terminal proliferation. These findings reveal an unanticipated scope of 
      the alternative splicing program and the importance of Mbnl1 during erythroid 
      terminal differentiation.
CI  - (c) 2014 by The American Society of Hematology.
FAU - Cheng, Albert W
AU  - Cheng AW
AD  - Whitehead Institute for Biomedical Research, Cambridge, MA; Computational and 
      Systems Biology Program, and.
FAU - Shi, Jiahai
AU  - Shi J
AUID- ORCID: 0000-0002-6467-8289
AD  - Whitehead Institute for Biomedical Research, Cambridge, MA;
FAU - Wong, Piu
AU  - Wong P
AD  - Whitehead Institute for Biomedical Research, Cambridge, MA;
FAU - Luo, Katherine L
AU  - Luo KL
AD  - Departments of Biology and Biological Engineering, Massachusetts Institute of 
      Technology, Cambridge, MA; and.
FAU - Trepman, Paula
AU  - Trepman P
AD  - Departments of Biology and Biological Engineering, Massachusetts Institute of 
      Technology, Cambridge, MA; and.
FAU - Wang, Eric T
AU  - Wang ET
AD  - Departments of Biology and Biological Engineering, Massachusetts Institute of 
      Technology, Cambridge, MA; and Harvard-MIT Division of Health Sciences and 
      Technology, Cambridge, MA.
FAU - Choi, Heejo
AU  - Choi H
AD  - Departments of Biology and Biological Engineering, Massachusetts Institute of 
      Technology, Cambridge, MA; and.
FAU - Burge, Christopher B
AU  - Burge CB
AD  - Departments of Biology and Biological Engineering, Massachusetts Institute of 
      Technology, Cambridge, MA; and.
FAU - Lodish, Harvey F
AU  - Lodish HF
AD  - Whitehead Institute for Biomedical Research, Cambridge, MA; Departments of 
      Biology and Biological Engineering, Massachusetts Institute of Technology, 
      Cambridge, MA; and.
LA  - eng
GR  - P01 HL032262/HL/NHLBI NIH HHS/United States
GR  - R01 DK068348/DK/NIDDK NIH HHS/United States
GR  - R01DK068348-07/DK/NIDDK NIH HHS/United States
GR  - P01 HL032262-25/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140528
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Mbnl1 protein, mouse)
RN  - 0 (Ndel1 protein, mouse)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
CIN - Blood. 2014 Jul 24;124(4):474-5. PMID: 25061168
MH  - *Alternative Splicing
MH  - Animals
MH  - Blotting, Western
MH  - Carrier Proteins/antagonists & inhibitors/*genetics/metabolism
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - DNA-Binding Proteins/antagonists & inhibitors/genetics/*metabolism
MH  - Erythropoiesis/*physiology
MH  - Exons/genetics
MH  - *Gene Expression Regulation
MH  - HEK293 Cells
MH  - Humans
MH  - Immunoprecipitation
MH  - Mice
MH  - Protein Isoforms
MH  - RNA Precursors/*genetics
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/genetics
MH  - RNA-Binding Proteins/antagonists & inhibitors/genetics/*metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC4110662
EDAT- 2014/05/30 06:00
MHDA- 2014/11/19 06:00
PMCR- 2015/07/24
CRDT- 2014/05/30 06:00
PHST- 2014/05/30 06:00 [entrez]
PHST- 2014/05/30 06:00 [pubmed]
PHST- 2014/11/19 06:00 [medline]
PHST- 2015/07/24 00:00 [pmc-release]
AID - S0006-4971(20)39941-9 [pii]
AID - 2013/542209 [pii]
AID - 10.1182/blood-2013-12-542209 [doi]
PST - ppublish
SO  - Blood. 2014 Jul 24;124(4):598-610. doi: 10.1182/blood-2013-12-542209. Epub 2014 
      May 28.