PMID- 24871532
OWN - NLM
STAT- MEDLINE
DCOM- 20150423
LR  - 20151119
IS  - 1569-9285 (Electronic)
IS  - 1569-9285 (Linking)
VI  - 19
IP  - 3
DP  - 2014 Sep
TI  - Effects of human adipose-derived stem cells on the regeneration of damaged 
      visceral pleural mesothelial cells: a morphological study in a rabbit model.
PG  - 363-7
LID - 10.1093/icvts/ivu124 [doi]
AB  - OBJECTIVES: Although an alveolar air leak (AAL) after pulmonary resection is a 
      troublesome complication that diminishes a patient's quality of life and 
      increases medical costs, current treatment and preventive methods for AAL are not 
      effective. Therefore, we transplanted adipose-derived stem cells (ASCs) to the 
      damaged visceral pleura to facilitate the regeneration of mesothelial cells and 
      investigated the possibility of cell therapy as a treatment option for AAL. 
      METHODS: Stem cells were isolated and cultured from discarded fat tissues that 
      were collected after liposuction procedures. Flow cytometry analysis was 
      performed to evaluate their suitability as mesenchymal stem cells. Cultured stem 
      cells were seeded onto polyglycolic acid (PGA) sheets and incubated for 5 days. 
      Under general anaesthesia, 10 New Zealand rabbits underwent thoracotomies. After 
      the visceral pleura was damaged, PGA sheets containing ASCs were transplanted 
      into 5 rabbits (ASC group) and PGA sheets without cells were transplanted into 
      the other 5 rabbits (control group). Rethoracotomies were performed after 4 
      weeks, and the transplanted areas in the visceral pleura were excised for 
      analysis. Haematoxylin and eosin and Azan staining were performed. In addition, 
      electron microscopic examinations were performed to investigate the 
      ultrastructure of the regenerating mesothelium. RESULTS: Cultured stem cells were 
      positive for the surface proteins CD13, CD29, CD49d, CD90 and CD105, whereas they 
      were negative for CD34, CD45 and human leukocyte antigen (HLA)-DR. The adhesions 
      between the transplanted visceral pleura and parietal pleura were weaker in the 
      ASC group than in the control group. On histological examination, the mesothelial 
      cell monolayer of the visceral pleura was well preserved in the ASC group, 
      whereas it was frequently lost in the control group. Electron microscopy 
      demonstrated that the mesothelial cell monolayer and its abundant microvilli were 
      well preserved in the ASC group, but were absent or disintegrated in the control 
      group. CONCLUSIONS: Transplantation of ASCs to the damaged visceral pleura can 
      contribute to the treatment and prevention of AAL by improving the regeneration 
      of mesothelial cells.
CI  - (c) The Author 2014. Published by Oxford University Press on behalf of the European 
      Association for Cardio-Thoracic Surgery. All rights reserved.
FAU - Kim, Young-Du
AU  - Kim YD
AD  - Department of Thoracic and Cardiovascular Surgery, College of Medicine, The 
      Catholic University of Korea, Seoul, Korea.
FAU - Jun, Young Joon
AU  - Jun YJ
AD  - Department of Plastic and Reconstruction Surgery, College of Medicine, The 
      Catholic University of Korea, Seoul, Korea.
FAU - Kim, Jeana
AU  - Kim J
AD  - Department of Pathology, College of Medicine, The Catholic University of Korea, 
      Seoul, Korea.
FAU - Kim, Chi Kyung
AU  - Kim CK
AD  - Department of Thoracic and Cardiovascular Surgery, College of Medicine, The 
      Catholic University of Korea, Seoul, Korea kckmd@hanmail.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140528
PL  - England
TA  - Interact Cardiovasc Thorac Surg
JT  - Interactive cardiovascular and thoracic surgery
JID - 101158399
RN  - 0 (Biomarkers)
RN  - 26009-03-0 (Polyglycolic Acid)
SB  - IM
CIN - Interact Cardiovasc Thorac Surg. 2014 Sep;19(3):367. PMID: 25125567
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Communication
MH  - Cell Culture Techniques
MH  - *Cell Proliferation
MH  - Cell Shape
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Epithelial Cells/metabolism/*pathology
MH  - Humans
MH  - Pleura/injuries/metabolism/pathology/*surgery
MH  - Pneumonectomy
MH  - Polyglycolic Acid
MH  - Rabbits
MH  - *Regeneration
MH  - *Stem Cell Transplantation
MH  - Stem Cells/metabolism/*physiology
MH  - Subcutaneous Fat, Abdominal/*cytology
MH  - Time Factors
MH  - Tissue Scaffolds
OTO - NOTNLM
OT  - Adipose-derived stem cell
OT  - Alveolar air leak
OT  - Mesothelial cell
EDAT- 2014/05/30 06:00
MHDA- 2015/04/24 06:00
CRDT- 2014/05/30 06:00
PHST- 2014/05/30 06:00 [entrez]
PHST- 2014/05/30 06:00 [pubmed]
PHST- 2015/04/24 06:00 [medline]
AID - ivu124 [pii]
AID - 10.1093/icvts/ivu124 [doi]
PST - ppublish
SO  - Interact Cardiovasc Thorac Surg. 2014 Sep;19(3):363-7. doi: 10.1093/icvts/ivu124. 
      Epub 2014 May 28.