PMID- 24872620
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140529
LR  - 20211021
IS  - 0972-124X (Print)
IS  - 0975-1580 (Electronic)
IS  - 0972-124X (Linking)
VI  - 18
IP  - 2
DP  - 2014 Mar
TI  - mRNA expression of pattern recognition receptors and their signaling mediators in 
      healthy and diseased gingival tissues.
PG  - 150-4
LID - 10.4103/0972-124X.131309 [doi]
AB  - BACKGROUND: Gingivitis and periodontitis are initiated by inflammation caused by 
      microorganisms. Pathogen-associated molecular patterns (PAMPs) from these 
      microorganisms are recognized through various toll-like receptors (TLRs) and 
      NOD-like receptors (NLRs). In this study, we have chosen five TLRs and two NLRs 
      as representatives taking part in the recognition and inflammation process, along 
      with a few of their signaling mediators including CD14, MYD88, and TRIF to 
      compare their mRNA expression levels between healthy and diseased gingival 
      tissues. This will provide deeper insight into the mechanisms underlying 
      gingivitis and periodontitis. Understanding the mechanisms involved in the onset 
      and progression of the periodontal diseases could greatly help in establishing 
      effective ways for prevention and treatment of these diseases besides decreasing 
      the risk factor for relevant systemic disorders. MATERIALS AND METHODS: Gingival 
      tissue samples for mRNA extraction and cDNA synthesis were taken from patients 
      with gingivitis and periodontitis and from healthy control subjects. Messenger 
      RNA expression of all genes was assessed using real-time polymerase chain 
      reaction (PCR). RESULTS: Among the genes studied in different groups, only MYD88 
      mRNA expression was significantly higher in the periodontitis group compared to 
      that of the controls. The expression level of this molecule was also 
      significantly higher in patients with severe periodontitis compared to other 
      patients and also compared to healthy individuals. In different tissues, positive 
      significant correlations were observed between the mRNA expression levels of some 
      genes. CONCLUSIONS: Elevated mRNA levels of MYD88 in periodontitis might have a 
      key role in the pathogenesis of this disease. Therefore, MYD88 may be a useful 
      target for the therapy of this disease.
FAU - Ghaderi, Hamid
AU  - Ghaderi H
AD  - Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, 
      Shiraz, Iran ; Department of Periodontology, School of Dentistry, Shiraz 
      University of Medical Sciences, Shiraz, Iran.
FAU - Kiany, Farin
AU  - Kiany F
AD  - Department of Periodontology, School of Dentistry, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Razmkhah, Mahboobeh
AU  - Razmkhah M
AD  - Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, 
      Shiraz, Iran.
FAU - Dadras, Somayeh
AU  - Dadras S
AD  - Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, 
      Shiraz, Iran.
FAU - Chenari, Noushafarin
AU  - Chenari N
AD  - Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, 
      Shiraz, Iran.
FAU - Hosseini, Ahmad
AU  - Hosseini A
AD  - Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, 
      Shiraz, Iran.
FAU - Younesi, Vahid
AU  - Younesi V
AD  - Department of Immunology, School of Medicine, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Ghaderi, Abbas
AU  - Ghaderi A
AD  - Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, 
      Shiraz, Iran ; Department of Immunology, School of Medicine, Shiraz University of 
      Medical Sciences, Shiraz, Iran.
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Indian Soc Periodontol
JT  - Journal of Indian Society of Periodontology
JID - 101499342
PMC - PMC4033878
OTO - NOTNLM
OT  - Gingivitis
OT  - inflammation
OT  - mRNA expression
OT  - periodontitis
OT  - toll-like receptor
COIS- Conflict of Interest: None declared.
EDAT- 2014/05/30 06:00
MHDA- 2014/05/30 06:01
PMCR- 2014/03/01
CRDT- 2014/05/30 06:00
PHST- 2013/06/23 00:00 [received]
PHST- 2013/10/07 00:00 [accepted]
PHST- 2014/05/30 06:00 [entrez]
PHST- 2014/05/30 06:00 [pubmed]
PHST- 2014/05/30 06:01 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - JISP-18-150 [pii]
AID - 10.4103/0972-124X.131309 [doi]
PST - ppublish
SO  - J Indian Soc Periodontol. 2014 Mar;18(2):150-4. doi: 10.4103/0972-124X.131309.