PMID- 24874199
OWN - NLM
STAT- MEDLINE
DCOM- 20150415
LR  - 20191210
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 189
DP  - 2014 Aug 30
TI  - The interaction between host Annexin A2 and viral Nsp9 is beneficial for 
      replication of porcine reproductive and respiratory syndrome virus.
PG  - 106-13
LID - S0168-1702(14)00219-6 [pii]
LID - 10.1016/j.virusres.2014.05.015 [doi]
AB  - Non-structural protein 9 (Nsp9), a RNA-dependent RNA polymerase (RdRp) of the 
      porcine reproductive and respiratory syndrome virus (PRRSV), is necessary for 
      PRRSV replication. However, the binding partners of Nsp9 have not been 
      identified. In this study, seven host proteins were identified as Nsp9-binding 
      proteins using yeast two-hybrid (Y2H). Among of them, we confirmed the 
      interaction of Nsp9 with Annexin A2 (ANXA2) using Y2H, Co-immunoprecipitation 
      (Co-IP), GST pulldown and immunofluorescence assay (IFA). We found that only 
      full-length ANXA2 could bind with Nsp9 in vitro and Nsp9 interacted with 
      endogenous ANXA2 in PRRSV-infected MARC-145 cells. In addition, we found that the 
      Nsp9-ANXA2 interaction was partially reduced by RNase A treatment. Furthermore, 
      PRRSV growth was significantly hindered in ANXA2-knockdown MARC-145 cells. Taken 
      together, these results indicate that Nsp9 binding partner ANXA2 is beneficial 
      for PRRSV replication.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Li, Jiangnan
AU  - Li J
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China.
FAU - Guo, Dongwei
AU  - Guo D
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing 163319, Heilongjiang, China.
FAU - Huang, Li
AU  - Huang L
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China.
FAU - Yin, Manman
AU  - Yin M
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China.
FAU - Liu, Qingfang
AU  - Liu Q
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China.
FAU - Wang, Yan
AU  - Wang Y
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China.
FAU - Yang, Chunmei
AU  - Yang C
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China.
FAU - Liu, Yuanyuan
AU  - Liu Y
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China.
FAU - Zhang, Lijie
AU  - Zhang L
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China.
FAU - Tian, Zhijun
AU  - Tian Z
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China.
FAU - Cai, Xuehui
AU  - Cai X
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China.
FAU - Yu, Liyun
AU  - Yu L
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing 163319, Heilongjiang, China.
FAU - Weng, Changjiang
AU  - Weng C
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, Heilongjiang, China. Electronic address: wengcj@hvri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140526
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Annexin A2)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Annexin A2/*metabolism
MH  - Cell Line
MH  - Centrifugation
MH  - Chlorocebus aethiops
MH  - Fluorescent Antibody Technique
MH  - *Host-Pathogen Interactions
MH  - Immunoprecipitation
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - *Protein Interaction Mapping
MH  - Two-Hybrid System Techniques
MH  - Viral Nonstructural Proteins/*metabolism
MH  - *Virus Replication
OTO - NOTNLM
OT  - Annexin A2 (ANXA2)
OT  - Non-structural protein 9 (Nsp9)
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Viral replication
OT  - Yeast two-hybrid (Y2H)
EDAT- 2014/05/31 06:00
MHDA- 2015/04/16 06:00
CRDT- 2014/05/31 06:00
PHST- 2014/01/29 00:00 [received]
PHST- 2014/05/06 00:00 [revised]
PHST- 2014/05/07 00:00 [accepted]
PHST- 2014/05/31 06:00 [entrez]
PHST- 2014/05/31 06:00 [pubmed]
PHST- 2015/04/16 06:00 [medline]
AID - S0168-1702(14)00219-6 [pii]
AID - 10.1016/j.virusres.2014.05.015 [doi]
PST - ppublish
SO  - Virus Res. 2014 Aug 30;189:106-13. doi: 10.1016/j.virusres.2014.05.015. Epub 2014 
      May 26.