PMID- 24875310
OWN - NLM
STAT- MEDLINE
DCOM- 20150812
LR  - 20181202
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 22
IP  - 10
DP  - 2015 May
TI  - Microbial diversity in innovative mesophilic/thermophilic temperature-phased 
      anaerobic digestion of sludge.
PG  - 7339-48
LID - 10.1007/s11356-014-3061-y [doi]
AB  - Anaerobic digestion (AD) is one of the few sustainable technologies that both 
      produce energy and treat waste streams. Driven by a complex and diverse community 
      of microbes, AD may be affected by different factors, many of which also 
      influence the composition and activity of the microbial community. In this study, 
      the biodiversity of microbial populations in innovative mesophilic/thermophilic 
      temperature-phased AD of sludge was evaluated by means of fluorescence in situ 
      hybridization (FISH). The increase of digestion temperature drastically affected 
      the microbial composition and selected specialized biomass. Hydrogenotrophic 
      Methanobacteriales and the protein fermentative bacterium Coprothermobacter spp. 
      were identified in the thermophilic anaerobic biomass. Shannon-Weaver diversity 
      (H') and evenness (E) indices were calculated using FISH data. Species richness 
      was lower under thermophilic conditions compared with the values estimated in 
      mesophilic samples, and it was flanked by similar trend of the evenness 
      indicating that thermophilic communities may be therefore more susceptible to 
      sudden changes and less prompt to adapting to operative variations.
FAU - Gagliano, M C
AU  - Gagliano MC
AD  - Water Research Institute, IRSA-CNR, Via Salaria km 29,300, Monterotondo, RM, 
      00015, Italy.
FAU - Braguglia, C M
AU  - Braguglia CM
FAU - Gallipoli, A
AU  - Gallipoli A
FAU - Gianico, A
AU  - Gianico A
FAU - Rossetti, S
AU  - Rossetti S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140531
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Sewage)
SB  - IM
MH  - Anaerobiosis
MH  - Bacteria/genetics/growth & development/isolation & purification/*metabolism
MH  - Biodegradation, Environmental
MH  - *Biodiversity
MH  - Biomass
MH  - Bioreactors/microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Sewage/*microbiology
MH  - Temperature
EDAT- 2014/05/31 06:00
MHDA- 2015/08/13 06:00
CRDT- 2014/05/31 06:00
PHST- 2014/03/07 00:00 [received]
PHST- 2014/05/19 00:00 [accepted]
PHST- 2014/05/31 06:00 [entrez]
PHST- 2014/05/31 06:00 [pubmed]
PHST- 2015/08/13 06:00 [medline]
AID - 10.1007/s11356-014-3061-y [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2015 May;22(10):7339-48. doi: 
      10.1007/s11356-014-3061-y. Epub 2014 May 31.