PMID- 24875922
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20140719
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 73
DP  - 2014 Aug
TI  - Proteolytic activity of Porphyromonas gingivalis attenuates MCP-1 mRNA expression 
      in LPS-stimulated THP-1 cells.
PG  - 13-8
LID - S0882-4010(14)00071-0 [pii]
LID - 10.1016/j.micpath.2014.05.004 [doi]
AB  - Bacteria can modulate cytokine production of host cells. In this study, we 
      examined effects of Porphyromonas gingivalis, an important periodontal pathogen, 
      on the cytokine production in lipopolysaccharide (LPS)-stimulated THP-1 
      macrophagic cells. A wide range of doses of P. gingivalis increased the tumor 
      necrosis factor-alpha (TNF-alpha) production. However, monocyte chemoattractant protein-1 
      (MCP-1) production was substantially suppressed by high doses of P. gingivalis 
      and this effect was demonstrated at the mRNA level. Challenges with a congenic 
      protease mutant strain did not significantly attenuate the MCP-1 mRNA expression 
      and addition of leupeptin, a protease inhibitor, to the cultures largely 
      prevented the inhibition of MCP-1 expression by P. gingivalis. Transwell 
      experiments showed that direct contact of P. gingivalis with THP-1 cells was not 
      required for the MCP-1 inhibition. Furthermore, blockade of internalization of P. 
      gingivalis into THP-1 cells had no effect on the MCP-1 inhibition by P. 
      gingivalis. Finally, degradation of MCP-1 mRNA in LPS-stimulated THP-1 cells was 
      accelerated in the presence of P. gingivalis. These results suggest that the 
      proteolytic activity of P. gingivalis attenuate MCP-1 mRNA expression by 
      promoting the decay of MCP-1 mRNA in LPS-stimulated THP-1 cells.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Choi, Eun-Kyoung
AU  - Choi EK
AD  - Department of Oral Microbiology, School of Dentistry, Chonnam National 
      University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
FAU - Kim, Sang-Yong
AU  - Kim SY
AD  - Department of Oral Microbiology, School of Dentistry, Chonnam National 
      University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
FAU - Kim, So-Hee
AU  - Kim SH
AD  - Department of Oral Microbiology, School of Dentistry, Chonnam National 
      University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
FAU - Paek, Yun-Woong
AU  - Paek YW
AD  - Department of Physical Therapy, Gwangju Health University, Gwangju 506-701, 
      Republic of Korea.
FAU - Kang, In-Chol
AU  - Kang IC
AD  - Department of Oral Microbiology, School of Dentistry, Chonnam National 
      University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea. 
      Electronic address: ickang@jnu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140527
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/*biosynthesis
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Immune Evasion
MH  - Lipopolysaccharides/*immunology
MH  - Monocytes/drug effects/*immunology/*microbiology
MH  - Porphyromonas gingivalis/*enzymology/*immunology
MH  - Proteolysis
MH  - RNA, Messenger/biosynthesis
OTO - NOTNLM
OT  - Lipopolysaccharide
OT  - Monocyte chemoattractant protein-1
OT  - Periodontitis
OT  - Porphyromonas gingivalis
OT  - Protease
OT  - THP-1
EDAT- 2014/05/31 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/05/31 06:00
PHST- 2014/04/03 00:00 [received]
PHST- 2014/05/14 00:00 [revised]
PHST- 2014/05/16 00:00 [accepted]
PHST- 2014/05/31 06:00 [entrez]
PHST- 2014/05/31 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - S0882-4010(14)00071-0 [pii]
AID - 10.1016/j.micpath.2014.05.004 [doi]
PST - ppublish
SO  - Microb Pathog. 2014 Aug;73:13-8. doi: 10.1016/j.micpath.2014.05.004. Epub 2014 
      May 27.