PMID- 24876407
OWN - NLM
STAT- MEDLINE
DCOM- 20150220
LR  - 20211021
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 91
IP  - 1
DP  - 2014 Jul
TI  - Age- and species-dependent infiltration of macrophages into the testis of rats 
      and mice exposed to mono-(2-Ethylhexyl) phthalate (MEHP).
PG  - 18
LID - 10.1095/biolreprod.113.115527 [doi]
LID - 18
AB  - The mechanism by which noninfectious testicular inflammation results in 
      infertility is poorly understood. Here the infiltration of CD11b+ immunoreactive 
      testicular interstitial cells (neutrophil, macrophages, dendritic cells) in 
      immature (Postnatal Day [PND] 21, 28, and 35) and adult (PND 56) Fischer rats is 
      described at 12, 24, and 48 h after an oral dose of 1 g/kg mono-(2-ethylhexyl) 
      phthalate (MEHP), a well-described Sertoli cell toxicant. Increases of CD11b+ 
      cells are evident 12 h after MEHP exposure in PND 21 and 28 rats. In PND 28 rats, 
      CD11b+ cells remained significantly elevated at 48 h, while in PND 21 rats, it 
      returned to control levels by 24 h. The peak number of CD11b+ cells in PND 35 rat 
      testis is delayed until 24 h, but remains significantly elevated at 48 h. In PND 
      56 rats, no increase in CD11b+ cells occurs after MEHP exposure. In PND 21, 28, 
      and 35 rats, a significant increase in monocyte chemoattractant protein-1 (MCP-1) 
      by peritubular myoid cells occurs 12 h after MEHP. Interestingly, MEHP treatment 
      of C57BL/6J mice did not incite an infiltration of CD11b+ cells at either PND 21 
      or 28. The peak level of germ cell apoptosis observed 24 h after MEHP exposure in 
      young rats is not seen in mice at any age or in PND 56 rats. Taken together, 
      these findings implicate MCP-1 released by peritubular myoid cells in provoking 
      the migration of CD11b+ cells into the immature rat testis early after MEHP 
      exposure and point to a role for CD11b+ cells in triggering germ cell apoptosis 
      in an age- and species-dependent manner.
CI  - (c) 2014 by the Society for the Study of Reproduction, Inc.
FAU - Murphy, Caitlin J
AU  - Murphy CJ
AD  - Center for Molecular and Cellular Toxicology, College of Pharmacy, The University 
      of Texas at Austin, Austin, Texas.
FAU - Stermer, Angela R
AU  - Stermer AR
AD  - Center for Molecular and Cellular Toxicology, College of Pharmacy, The University 
      of Texas at Austin, Austin, Texas.
FAU - Richburg, John H
AU  - Richburg JH
AD  - Center for Molecular and Cellular Toxicology, College of Pharmacy, The University 
      of Texas at Austin, Austin, Texas john.richburg@austin.utexas.edu.
LA  - eng
GR  - ES007784/ES/NIEHS NIH HHS/United States
GR  - ES016591/ES/NIEHS NIH HHS/United States
GR  - R01 ES016591/ES/NIEHS NIH HHS/United States
GR  - P30 ES007784/ES/NIEHS NIH HHS/United States
GR  - T32 ES007247/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140529
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Chemokine CCL2)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
RN  - FU2EWB60RT (mono-(2-ethylhexyl)phthalate)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Cell Movement/physiology
MH  - Chemokine CCL2/metabolism
MH  - Diethylhexyl Phthalate/*analogs & derivatives/pharmacology
MH  - Macrophages/*drug effects/immunology/metabolism
MH  - Male
MH  - Mice
MH  - Rats
MH  - Sertoli Cells/*drug effects/immunology/metabolism
MH  - Species Specificity
MH  - Testis/*drug effects/immunology/metabolism
PMC - PMC4434960
OTO - NOTNLM
OT  - interstitial cells
OT  - macrophage
OT  - myoid cells
OT  - reproductive immunology
OT  - toxicology
EDAT- 2014/05/31 06:00
MHDA- 2015/02/24 06:00
PMCR- 2015/07/01
CRDT- 2014/05/31 06:00
PHST- 2014/05/31 06:00 [entrez]
PHST- 2014/05/31 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - biolreprod.113.115527 [pii]
AID - 10.1095/biolreprod.113.115527 [doi]
PST - ppublish
SO  - Biol Reprod. 2014 Jul;91(1):18. doi: 10.1095/biolreprod.113.115527. Epub 2014 May 
      29.