PMID- 24877714
OWN - NLM
STAT- MEDLINE
DCOM- 20150420
LR  - 20191210
IS  - 1618-095X (Electronic)
IS  - 0944-7113 (Linking)
VI  - 21
IP  - 10
DP  - 2014 Sep 15
TI  - Effect of Tenuifoliside A isolated from Polygala tenuifolia on the ERK and PI3K 
      pathways in C6 glioma cells.
PG  - 1178-88
LID - S0944-7113(14)00206-2 [pii]
LID - 10.1016/j.phymed.2014.04.022 [doi]
AB  - Tenuifoliside A (TFSA) is a bioactive oligosaccharide ester component of Polygala 
      tenuifolia Wild, a traditional Chinese medicine which was used to manage mental 
      disorders effectively. The neuroprotective and anti-apoptotic effects of TFSA 
      have been demonstrated in our previous studies. The present work was designed to 
      study the molecular mechanism of TFSA on promoting the viability of rat glioma 
      cells C6. We exposed C6 cells to TFSA (or combined with ERK, PI3K and TrkB 
      inhibitors) to examine the effects of TFSA on the cell viability and the 
      expression and phosphorylation of key proteins in the ERK and PI3K signaling 
      pathway. TFSA increased levels of phospho-ERK and phospho-Akt, enhanced release 
      of BDNF, which were blocked by ERK and PI3K inhibitors, respectively (U0126 and 
      LY294002). Moreover, the TFSA caused the enhanced phosphorylation of cyclic AMP 
      response element binding protein (CREB) at Ser133 site, the effect was revoked by 
      U0126, LY294002 and K252a. Furthermore, when C6 cells were pretreated with K252a, 
      a TrkB antagonist, known to significantly inhibit the activity of brain-derived 
      neurotrophic factor (BDNF), blocked the levels of phospho-ERK, phospho-Akt and 
      phosphor-CREB. Taking these results together, we suggested the neuroprotection of 
      TFSA might be mediated through BDNF/TrkB-ERK/PI3K-CREB signaling pathway in C6 
      glioma cells.
CI  - Copyright (c) 2014 Elsevier GmbH. All rights reserved.
FAU - Dong, Xian-zhe
AU  - Dong XZ
AD  - Department of Clinical Pharmacology, Chinese PLA General Hospital, Beijing 
      100853, China. Electronic address: dongxianzhe301@163.com.
FAU - Huang, Cui-li
AU  - Huang CL
AD  - Department of Clinical Pharmacology, Chinese PLA General Hospital, Beijing 
      100853, China.
FAU - Yu, Bing-ying
AU  - Yu BY
AD  - Department of Clinical Pharmacology, Chinese PLA General Hospital, Beijing 
      100853, China; Department of Pharmacy, Hebei North University, Zhangjiakou 
      075000, China.
FAU - Hu, Yuan
AU  - Hu Y
AD  - Department of Clinical Pharmacology, Chinese PLA General Hospital, Beijing 
      100853, China.
FAU - Mu, Li-hua
AU  - Mu LH
AD  - Department of Clinical Pharmacology, Chinese PLA General Hospital, Beijing 
      100853, China.
FAU - Liu, Ping
AU  - Liu P
AD  - Department of Clinical Pharmacology, Chinese PLA General Hospital, Beijing 
      100853, China. Electronic address: cpi301@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140528
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Butadienes)
RN  - 0 (Carbazoles)
RN  - 0 (Chromones)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indole Alkaloids)
RN  - 0 (Morpholines)
RN  - 0 (Nitriles)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (U 0126)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - 97161-97-2 (staurosporine aglycone)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (Crebbp protein, rat)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.2.1.- (Disaccharidases)
RN  - S36S8L9Y70 (tenuifoliside A)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/drug effects
MH  - Butadienes/pharmacology
MH  - CREB-Binding Protein/antagonists & inhibitors/drug effects
MH  - Carbazoles/pharmacology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Chromones/pharmacology
MH  - Disaccharidases/chemistry/isolation & purification/*pharmacology
MH  - Drugs, Chinese Herbal/chemistry/isolation & purification/*pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Indole Alkaloids/pharmacology
MH  - MAP Kinase Signaling System/*drug effects
MH  - Models, Biological
MH  - Morpholines/pharmacology
MH  - Nitriles/pharmacology
MH  - Phosphatidylinositol 3-Kinases/*drug effects
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphorylation/drug effects
MH  - Plant Roots/chemistry
MH  - Polygala/*chemistry
MH  - Rats
MH  - Receptor, trkB/drug effects
MH  - Signal Transduction/*drug effects
OTO - NOTNLM
OT  - C6 glioma cells
OT  - PI3K pathways
OT  - Polygala tenuifolia
EDAT- 2014/06/01 06:00
MHDA- 2015/04/22 06:00
CRDT- 2014/06/01 06:00
PHST- 2013/10/30 00:00 [received]
PHST- 2014/03/04 00:00 [revised]
PHST- 2014/04/20 00:00 [accepted]
PHST- 2014/06/01 06:00 [entrez]
PHST- 2014/06/01 06:00 [pubmed]
PHST- 2015/04/22 06:00 [medline]
AID - S0944-7113(14)00206-2 [pii]
AID - 10.1016/j.phymed.2014.04.022 [doi]
PST - ppublish
SO  - Phytomedicine. 2014 Sep 15;21(10):1178-88. doi: 10.1016/j.phymed.2014.04.022. 
      Epub 2014 May 28.