PMID- 24877848
OWN - NLM
STAT- MEDLINE
DCOM- 20150407
LR  - 20140728
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 155
IP  - 1
DP  - 2014 Aug 8
TI  - Neuroprotective effect of 1-methoxyoctadecan-1-ol from Uncaria sinensis on 
      glutamate-induced hippocampal neuronal cell death.
PG  - 293-9
LID - S0378-8741(14)00396-1 [pii]
LID - 10.1016/j.jep.2014.05.027 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: We isolated a single compound, 
      1-methoxyoctadecan-1-ol (MOD), from dried hooks and stems of Uncaria sinensis, 
      which is used in traditional Korean medicine to provide relief from various 
      nervous related symptoms. MATERIALS AND METHODS: Neuroprotective effects of MOD 
      against glutamate-induced oxidative stress in HT22 cells were investigated by 
      analyzing cell viability, lactate dehydrogenase, flow cytometry, reactive oxygen 
      species (ROS) and Western blot assays. RESULTS: Exposure to glutamate alone 
      resulted in remarkable hippocampal neuronal cell death; however, pretreatment 
      with MOD resulted in suppression of neuronal death and ROS accumulation in 
      connection with cellular Ca2+ level after exposure to glutamate. Stimulation by 
      glutamate also caused significant protein level of phosphorylated p38 
      mitogen-activated protein kinases (MAPK), and dephosphorylated 
      phosphatidylinositol-3 kinase (PI3K), however, pretreatment with MOD resulted in 
      inhibition of these changes in protein level. Treatment with glutamate alone led 
      to suppressed protein level of mature brain-derived neurotrophic factor (BDNF) 
      and phosphorylated cAMP response element binding protein (CREB); however, 
      pretreatment with MOD resulted in significant enhancement of this level of 
      protein. Anti-oxidant N-acetyl-L-cysteine and both Ca2+ inhibitors, BAPTA and 
      EGTA, showed effects similar to those of MOD in all proteins examined, except 
      mature BDNF. CONCLUSIONS: Our results suggest that MOD mainly exerted 
      neuroprotective effects in suppression of ROS accumulation and up-regulation of 
      mature BDNF in association with p38 MAPK and PI3K signaling in hippocampal 
      neuronal cells.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - Ahn, Sung Min
AU  - Ahn SM
AD  - Department of Korean Medical Science, School of Korean Medicine, Pusan National 
      University, Yangsan 626-870, Republic of Korea.
FAU - Kim, Ha Neui
AU  - Kim HN
AD  - Department of Korean Medical Science, School of Korean Medicine, Pusan National 
      University, Yangsan 626-870, Republic of Korea.
FAU - Kim, Yu Ri
AU  - Kim YR
AD  - Department of Korean Medical Science, School of Korean Medicine, Pusan National 
      University, Yangsan 626-870, Republic of Korea.
FAU - Oh, Eun Young
AU  - Oh EY
AD  - Department of Korean Medical Science, School of Korean Medicine, Pusan National 
      University, Yangsan 626-870, Republic of Korea.
FAU - Choi, Young Whan
AU  - Choi YW
AD  - Department of Horticultural Bioscience, College of Natural Resource and Life 
      Science, Pusan National University, Miryang 626-706, Republic of Korea.
FAU - Shin, Hwa Kyoung
AU  - Shin HK
AD  - Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan 
      National University, Yangsan 626-870, Republic of Korea.
FAU - Choi, Byung Tae
AU  - Choi BT
AD  - Department of Korean Medical Science, School of Korean Medicine, Pusan National 
      University, Yangsan 626-870, Republic of Korea; Division of Meridian and 
      Structural Medicine, School of Korean Medicine, Pusan National University, 
      Yangsan 626-870, Republic of Korea. Electronic address: choibt@pusan.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140528
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (1-methoxyoctadecan-1-ol)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fatty Alcohols)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Death/drug effects
MH  - Fatty Alcohols/isolation & purification/*pharmacology
MH  - Flow Cytometry
MH  - Glutamic Acid/administration & dosage
MH  - Hippocampus/*drug effects/pathology
MH  - Medicine, Korean Traditional
MH  - Mice
MH  - Neuroprotective Agents/isolation & purification/*pharmacology
MH  - Oxidative Stress/drug effects
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
MH  - Uncaria/*chemistry
MH  - Up-Regulation/drug effects
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - 1-methoxyoctadecan-1-ol
OT  - Hippocampal cell
OT  - Oxidative stress
OT  - Protein kinases
OT  - Uncaria sinensis
EDAT- 2014/06/01 06:00
MHDA- 2015/04/08 06:00
CRDT- 2014/06/01 06:00
PHST- 2014/02/05 00:00 [received]
PHST- 2014/04/19 00:00 [revised]
PHST- 2014/05/19 00:00 [accepted]
PHST- 2014/06/01 06:00 [entrez]
PHST- 2014/06/01 06:00 [pubmed]
PHST- 2015/04/08 06:00 [medline]
AID - S0378-8741(14)00396-1 [pii]
AID - 10.1016/j.jep.2014.05.027 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2014 Aug 8;155(1):293-9. doi: 10.1016/j.jep.2014.05.027. Epub 
      2014 May 28.