PMID- 24878561
OWN - NLM
STAT- MEDLINE
DCOM- 20150527
LR  - 20211021
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 21
IP  - 18
DP  - 2014 Sep
TI  - Environmental concentrations of 3,4-methylenedioxymethamphetamine (MDMA)-induced 
      cellular stress and modulated antioxidant enzyme activity in the zebra mussel.
PG  - 11099-106
LID - 10.1007/s11356-014-3094-2 [doi]
AB  - Recent monitoring studies showed measurable levels of the 
      3,4-methylenedioxymethamphetamine (MDMA) in aquatic environments. However, no 
      information is currently available on its potential hazard to aquatic non-target 
      organisms. The aim of this study was to investigate the potential sub-lethal 
      effects induced by 14-day exposures to low MDMA concentrations (0.05 and 0.5 
      mug/L) to zebra mussel (Dreissena polymorpha) specimens through the application of 
      a biomarker suite. The trypan blue exclusion method and the neutral red retention 
      assay (NRRA) were used to assess MDMA cytotoxicity. The activity of superoxide 
      dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione 
      S-transferase (GST), as well as the lipid peroxidation (LPO) and protein carbonyl 
      content (PCC), were measured as oxidative stress indexes. The single cell gel 
      electrophoresis (SCGE) assay, the DNA diffusion assay, and the micronucleus test 
      (MN test) were applied to investigate DNA damage, while filtration rate was 
      measured as physiological parameter. Despite significant decrease in lysosome 
      membrane stability, hemocyte viability and imbalances in CAT and GST activities 
      pointed out at the end of the exposure to 0.5 mug/L, no significant variations for 
      the other end points were noticed at both the treatments, suggesting that 
      environmentally relevant MDMA concentrations did not induce deleterious effects 
      to the zebra mussel.
FAU - Parolini, Marco
AU  - Parolini M
AD  - Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, 
      Italy, marco.parolini@unimi.it.
FAU - Magni, Stefano
AU  - Magni S
FAU - Binelli, Andrea
AU  - Binelli A
LA  - eng
PT  - Journal Article
DEP - 20140601
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Water Pollutants, Chemical)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Catalase/metabolism
MH  - Cell Survival
MH  - Cells, Cultured
MH  - DNA Damage
MH  - Dreissena/drug effects/*enzymology
MH  - Glutathione Peroxidase/metabolism
MH  - Glutathione Transferase/metabolism
MH  - Hemocytes/drug effects
MH  - Lipid Peroxidation/drug effects
MH  - N-Methyl-3,4-methylenedioxyamphetamine/analysis/*toxicity
MH  - Oxidative Stress
MH  - Protein Carbonylation
MH  - Superoxide Dismutase/metabolism
MH  - Water Pollutants, Chemical/analysis/*toxicity
MH  - Water Pollution, Chemical
EDAT- 2014/06/01 06:00
MHDA- 2015/05/28 06:00
CRDT- 2014/06/01 06:00
PHST- 2014/04/09 00:00 [received]
PHST- 2014/05/23 00:00 [accepted]
PHST- 2014/06/01 06:00 [entrez]
PHST- 2014/06/01 06:00 [pubmed]
PHST- 2015/05/28 06:00 [medline]
AID - 10.1007/s11356-014-3094-2 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2014 Sep;21(18):11099-106. doi: 
      10.1007/s11356-014-3094-2. Epub 2014 Jun 1.