PMID- 24879005
OWN - NLM
STAT- MEDLINE
DCOM- 20140831
LR  - 20211021
IS  - 1678-9946 (Electronic)
IS  - 0036-4665 (Print)
IS  - 0036-4665 (Linking)
VI  - 56
IP  - 3
DP  - 2014 May-Jun
TI  - Dose response effect of Paracoccidioides brasiliensis in an experimental model of 
      arthritis.
PG  - 259-64
LID - S0036-46652014000300259 [pii]
AB  - Paracoccidioidomycosis (PCM) is caused by the dimorphic fungus Paracoccidioides 
      brasiliensis (Pb) and corresponds to prevalent systemic mycosis in Latin America. 
      The aim of the present work was to evaluate the dose response effect of the 
      fungal yeast phase for the standardization of an experimental model of septic 
      arthritis. The experiments were performed with groups of 14 rats that received 
      doses of 103, 104 or 105 P. brasiliensis (Pb18) cells. The fungi were injected in 
      50 microL of phosphate-buffered saline (PBS) directly into the knee joints of the 
      animals. The following parameters were analyzed in this work: the formation of 
      swelling in knees infused with yeast cells and the radiological and 
      anatomopathological alterations, besides antibody titer by ELISA. After 15 days 
      of infection, signs of inflammation were evident. At 45 days, some features of 
      damage and necrosis were observed in the articular cartilage. The systemic 
      dissemination of the fungus was observed in 11% of the inoculated animals, and it 
      was concluded that the experimental model is able to mimic articular PCM in 
      humans and that the dose of 105 yeast cells can be used as standard in this 
      model.
FAU - Loth, Eduardo Alexandre
AU  - Loth EA
AD  - Centro de Ciencias Biologicas e da Saude, Universidade Estadual do Oeste do 
      Parana, Cascavel, Parana, PR, Brazil.
FAU - Biazim, Samia Khalil
AU  - Biazim SK
AD  - Centro de Ciencias Biologicas e da Saude, Universidade Estadual do Oeste do 
      Parana, Cascavel, Parana, PR, Brazil.
FAU - Dos Santos, Jose Henrique Fermino Ferreira
AU  - Dos Santos JH
AD  - Centro de Ciencias Biologicas e da Saude, Universidade Estadual do Oeste do 
      Parana, Cascavel, Parana, PR, Brazil.
FAU - Puccia, Rosana
AU  - Puccia R
AD  - Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal 
      de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Brancalhao, Rosimeire Costa
AU  - Brancalhao RC
AD  - Centro de Ciencias Biologicas e da Saude, Universidade Estadual do Oeste do 
      Parana, Cascavel, Parana, PR, Brazil.
FAU - Chasco, Lucineia de Fatima
AU  - Chasco Lde F
AD  - Centro de Ciencias Biologicas e da Saude, Universidade Estadual do Oeste do 
      Parana, Cascavel, Parana, PR, Brazil.
FAU - Gandra, Rinaldo Ferreira
AU  - Gandra RF
AD  - Centro de Ciencias Medicas e Farmaceuticas, Hospital Universitario do Oeste do 
      Parana, Universidade Estadual do Oeste do Parana, Cascavel, PR, Brazil.
FAU - Simao, Rita de Cassia Garcia
AU  - Simao Rde C
AD  - Centro de Ciencias Medicas e Farmaceuticas, Hospital Universitario do Oeste do 
      Parana, Universidade Estadual do Oeste do Parana, Cascavel, PR, Brazil.
FAU - de Franco, Marcello Fabiano
AU  - de Franco MF
AD  - Departamento de Patologia, Universidade Federal de Sao Paulo, Sao Paulo, SP, 
      Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Rev Inst Med Trop Sao Paulo
JT  - Revista do Instituto de Medicina Tropical de Sao Paulo
JID - 7507484
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/*microbiology/pathology
MH  - Arthritis, Infectious/*microbiology/pathology
MH  - Arthrography
MH  - Histocytochemistry
MH  - Male
MH  - Paracoccidioides/*pathogenicity
MH  - Paracoccidioidomycosis/*microbiology/pathology
MH  - Rats
MH  - Rats, Wistar
PMC - PMC4085864
EDAT- 2014/06/01 06:00
MHDA- 2014/09/01 06:00
PMCR- 2014/05/01
CRDT- 2014/06/01 06:00
PHST- 2013/08/02 00:00 [received]
PHST- 2013/10/23 00:00 [accepted]
PHST- 2014/06/01 06:00 [entrez]
PHST- 2014/06/01 06:00 [pubmed]
PHST- 2014/09/01 06:00 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - S0036-46652014000300259 [pii]
AID - 10.1590/s0036-46652014000300012 [doi]
PST - ppublish
SO  - Rev Inst Med Trop Sao Paulo. 2014 May-Jun;56(3):259-64. doi: 
      10.1590/s0036-46652014000300012.