PMID- 24879443
OWN - NLM
STAT- MEDLINE
DCOM- 20150512
LR  - 20211021
IS  - 1567-2387 (Electronic)
IS  - 1567-2379 (Linking)
VI  - 45
IP  - 5
DP  - 2014 Oct
TI  - Expression of epidermal fatty acid binding protein (E-FABP) in septoclasts in the 
      growth plate cartilage of mice.
PG  - 507-18
LID - 10.1007/s10735-014-9576-1 [doi]
AB  - n-3 Polyunsaturated fatty acids play a role in regulating the growth of the long 
      bones. Fatty acid-binding proteins (FABPs) bind and transport hydrophobic 
      long-chain fatty acids intracellularly, and epidermal-type FABP (E-FABP) has an 
      affinity for n-3 fatty acids. This study aimed to clarify the localization of 
      E-FABP in the growth plate of the mouse tibia. At the chondro-osseous junction 
      (COJ) of the growth plate, E-FABP-immunoreactivity was exclusively localized in 
      mononuclear, spindle-shaped cells with several long processes. These 
      E-FABP-immunoreactive cells were identified as being septoclasts, i.e., cells 
      that resorb uncalcified transverse septa. The processes of these immunoreactive 
      septoclasts terminated between the longitudinal and transverse septa. 
      E-FABP-immunoreactivity was found in the entire cytoplasm and on the 
      mitochondrial outer membrane. In ontogeny, immunoreactive septoclasts were 
      observed immediately after emergence of the primary ossifying center and were 
      distributed not only at the COJ but also in the metaphysis near the COJ. The 
      number of septoclasts increased at the postnatal age of 1 week (P1w)-P2w, and 
      thereafter gradually decreased; and the cells became concentrated at the COJ 
      after P3w-P4w. The immunoreactivity for peroxisome proliferator-activated 
      receptor (PPAR)beta/delta was detected in these E-FABP-immunoreactive septoclasts. The 
      present results suggest that fatty acids, preferably n-3 ones, are 
      intracellularly transported by E-FABP to various targets, including mitochondria 
      and nucleus, in which PPARbeta/delta may play functional roles in the transcriptional 
      regulation of genes involved in the endochondral ossification.
FAU - Bando, Yasuhiko
AU  - Bando Y
AD  - Division of Anatomy, Meikai University School of Dentistry, 1-1 Keyakidai, 
      Sakado, Saitama, 3500283, Japan, y-bando@dent.meikai.ac.jp.
FAU - Yamamoto, Miyuki
AU  - Yamamoto M
FAU - Sakiyama, Koji
AU  - Sakiyama K
FAU - Inoue, Katsuyuki
AU  - Inoue K
FAU - Takizawa, Shota
AU  - Takizawa S
FAU - Owada, Yuji
AU  - Owada Y
FAU - Iseki, Shoichi
AU  - Iseki S
FAU - Kondo, Hisatake
AU  - Kondo H
FAU - Amano, Osamu
AU  - Amano O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140531
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - 0 (Fabp5 protein, mouse)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (PPAR delta)
RN  - 0 (PPAR gamma)
SB  - IM
MH  - Animals
MH  - Cytoplasm/metabolism
MH  - Fatty Acid-Binding Proteins/*genetics/metabolism
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Growth Plate/cytology/growth & development/*metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Microscopy, Confocal
MH  - Microscopy, Immunoelectron
MH  - Mitochondrial Membranes/metabolism
MH  - Neoplasm Proteins/*genetics/metabolism
MH  - PPAR delta/metabolism
MH  - PPAR gamma/metabolism
MH  - Pregnancy
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tibia/cytology/growth & development/*metabolism
MH  - Time Factors
EDAT- 2014/06/01 06:00
MHDA- 2015/05/13 06:00
CRDT- 2014/06/01 06:00
PHST- 2014/05/08 00:00 [received]
PHST- 2014/05/21 00:00 [accepted]
PHST- 2014/06/01 06:00 [entrez]
PHST- 2014/06/01 06:00 [pubmed]
PHST- 2015/05/13 06:00 [medline]
AID - 10.1007/s10735-014-9576-1 [doi]
PST - ppublish
SO  - J Mol Histol. 2014 Oct;45(5):507-18. doi: 10.1007/s10735-014-9576-1. Epub 2014 
      May 31.