PMID- 24880628
OWN - NLM
STAT- MEDLINE
DCOM- 20150512
LR  - 20140828
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 98
IP  - 18
DP  - 2014 Sep
TI  - Cultivation of nitrite-dependent anaerobic methane-oxidizing bacteria: impact of 
      reactor configuration.
PG  - 7983-91
LID - 10.1007/s00253-014-5835-z [doi]
AB  - Nitrite-dependent anaerobic methane oxidation (n-damo) is mediated by bacteria 
      that anaerobically oxidize methane coupled with nitrite reduction and is a 
      potential bioprocess for wastewater treatment. In this work, the effect of 
      reactor configuration on n-damo bacterial cultivation was investigated. A 
      magnetically stirred gas lift reactor (MSGLR), a sequencing batch reactor (SBR), 
      and a continuously stirred tank reactor (CSTR) were selected to cultivate the 
      bacteria. Microbial community was monitored by using quantitative PCR, 16S rRNA 
      gene sequencing, pmoA gene sequencing, and fluorescence in situ hybridization 
      (FISH). The effects of substrate inhibition, methane mass transfer, and biomass 
      washout in the three reactors were focused on. The results indicated that the 
      MSGLR had the best performance among the three reactor systems, with the highest 
      total and specific n-damo activities. Its maximum volumetric nitrogen removal 
      rate was up to 76.9 mg N L(-1) day(-1), which was higher than previously reported 
      values (5.1-37.8 mg N L(-1) d(-1)).
FAU - Hu, Baolan
AU  - Hu B
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China.
FAU - He, Zhanfei
AU  - He Z
FAU - Geng, Sha
AU  - Geng S
FAU - Cai, Chen
AU  - Cai C
FAU - Lou, Liping
AU  - Lou L
FAU - Zheng, Ping
AU  - Zheng P
FAU - Xu, Xinhua
AU  - Xu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140601
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Nitrites)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Anaerobiosis
MH  - Bioreactors/microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Methylococcaceae/genetics/*metabolism
MH  - Nitrites/*metabolism
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2014/06/02 06:00
MHDA- 2015/05/13 06:00
CRDT- 2014/06/02 06:00
PHST- 2014/03/25 00:00 [received]
PHST- 2014/05/15 00:00 [accepted]
PHST- 2014/05/14 00:00 [revised]
PHST- 2014/06/02 06:00 [entrez]
PHST- 2014/06/02 06:00 [pubmed]
PHST- 2015/05/13 06:00 [medline]
AID - 10.1007/s00253-014-5835-z [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2014 Sep;98(18):7983-91. doi: 
      10.1007/s00253-014-5835-z. Epub 2014 Jun 1.