PMID- 24881272
OWN - NLM
STAT- MEDLINE
DCOM- 20150716
LR  - 20140602
IS  - 0005-9366 (Print)
IS  - 0005-9366 (Linking)
VI  - 127
IP  - 5-6
DP  - 2014 May-Jun
TI  - Comparative evaluation of serum, FTA filter-dried blood and oral fluid as sample 
      material for PRRSV diagnostics by RT-qPCR in a small-scale experimental study.
PG  - 216-21
AB  - Recently, research into alternative sample materials, such as oral fluid or 
      filter-dried blood has been intensified, in order to facilitate cost-effective 
      and animal-friendly sampling of individuals or groups of pigs for diagnostic 
      purposes. The objective of this study was to compare the sensitivity of porcine 
      reproductive and respiratory syndrome virus (PRRSV)-RNA detection by reverse 
      transcription quantitative real-time PCR (RT-qPCR) in serum, FTA filter-dried 
      blood and oral fluid sampled from individual pigs. Ten PRRSV negative pigs were 
      injected with an EU-type PRRSV live vaccine. Blood and oral fluid samples were 
      taken from each pig before, and 4, 7, 14 and 21 days after vaccination. All 
      samples were then analyzed by PRRSV RT-qPCR. In serum, eight often pigs tested 
      RT-qPCR positive at different time points post infection. Absolute quantification 
      showed low serum PRRSV-RNA loads in most samples. In comparison to serum, 
      sensitivity of PRRSV-RNA detection was strongly reduced in matched FTA 
      filter-dried blood and in oral fluid from the same pigs. These results indicate 
      that with low PRRSV-RNA loads the diagnostic sensitivity of PRRSV-RNA detection 
      by RT-qPCR achieved with serum is currently unmatched by either FTA filter-dried 
      blood or oral fluid.
FAU - Steinrigl, Adolf
AU  - Steinrigl A
FAU - Revilla-Fernandez, Sandra
AU  - Revilla-Fernandez S
FAU - Wodak, Eveline
AU  - Wodak E
FAU - Schmoll, Friedrich
AU  - Schmoll F
FAU - Sattler, Tatjana
AU  - Sattler T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Berl Munch Tierarztl Wochenschr
JT  - Berliner und Munchener tierarztliche Wochenschrift
JID - 0003163
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Blood Specimen Collection/methods/standards/*veterinary
MH  - Cost-Benefit Analysis
MH  - Dried Blood Spot Testing/standards/*veterinary
MH  - Enzyme-Linked Immunosorbent Assay/standards/veterinary
MH  - Female
MH  - Male
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - RNA, Viral/blood/*isolation & purification
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Saliva/*virology
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2014/06/03 06:00
MHDA- 2015/07/17 06:00
CRDT- 2014/06/03 06:00
PHST- 2014/06/03 06:00 [entrez]
PHST- 2014/06/03 06:00 [pubmed]
PHST- 2015/07/17 06:00 [medline]
PST - ppublish
SO  - Berl Munch Tierarztl Wochenschr. 2014 May-Jun;127(5-6):216-21.