PMID- 24882274
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20140726
IS  - 1879-2472 (Electronic)
IS  - 0049-3848 (Linking)
VI  - 134
IP  - 2
DP  - 2014 Aug
TI  - oxLDL/beta2GPI/anti-beta2GPI complex induced macrophage differentiation to foam cell 
      involving TLR4/NF-kappa B signal transduction pathway.
PG  - 384-92
LID - S0049-3848(14)00282-5 [pii]
LID - 10.1016/j.thromres.2014.05.017 [doi]
AB  - Macrophage-derived foam cell formation is a hallmark of atherosclerosis. It has 
      been reported that oxidized low density lipoprotein (oxLDL) inducing formation of 
      foam cells and expression of inflammatory molecules are partly mediated by 
      toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-kappaB) pathway. However, 
      whether oxLDL/beta2-glycoprotein I/anti-beta2-glycoprotein I (oxLDL/beta2GPI/anti-beta2GPI) 
      complex enhanced formation of foam cells involving TLR4/NF-kappaB pathway or not has 
      never been explored. In the current study, we focused on investigating the 
      transformation of peritoneal macrophages from BALB/c mice into foam cells induced 
      by the three complexes, and the involvement of TLR4 as well as its downstream 
      signal molecule NF-kappaB. The results showed that treatment of macrophages with 
      oxLDL/beta2GPI/anti-beta2GPI complex could markedly increase intracellular lipid 
      loading and expression of TLR4, phosphorylated NF-kappaB p65 (p-NF-kappaB p65), monocyte 
      chemoattractant protein-1 (MCP-1), as well as tissue factor (TF). The oxLDL and 
      oxLDL/beta2GPI/anti-beta2GPI complex induced formation of foam cells and expression of 
      p-NF-kappaB p65 were significantly reduced, while macrophages were pre-treated with 
      TLR4 inhibitor TAK-242. Meanwhile, both TAK-242 and NF-kappaB inhibitor PDTC could 
      remarkably inhibit oxLDL, oxLDL/beta2GPI/anti-beta2GPI complex, as well as LPS 
      increased MCP-1 and TF levels. Nevertheless, beta2GPI/anti-beta2GPI complex-induced 
      MCP-1 and TF mRNA expression were inhibited by TAK-242 rather than PDTC, although 
      TF activity was significantly reduced by both of the inhibitors. In conclusion, 
      our results indicate that oxLDL/beta2GPI/anti-beta2GPI complex could enhance the 
      conversion of macrophages into foam cells and the process may be at least partly 
      mediated by TLR4/NF-kappaB pathway, which may contribute to the accelerated 
      development of atherosclerosis in APS.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Xu, Ya
AU  - Xu Y
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, School of 
      Medical Science and Laboratory Medicine of Jiangsu University, Zhenjiang, Jiangsu 
      212013, PR China; Department of Clinical Laboratory and Hematology, School of 
      Medical Science and Laboratory Medicine of Jiangsu University, Zhenjiang, Jiangsu 
      212013, PR China; The First Affiliated Hospital of Soochow University, Suzhou 
      First People's Hospital.
FAU - Kong, Xiangmin
AU  - Kong X
AD  - Department of Clinical Laboratory and Hematology, School of Medical Science and 
      Laboratory Medicine of Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, School of 
      Medical Science and Laboratory Medicine of Jiangsu University, Zhenjiang, Jiangsu 
      212013, PR China; Department of Clinical Laboratory and Hematology, School of 
      Medical Science and Laboratory Medicine of Jiangsu University, Zhenjiang, Jiangsu 
      212013, PR China. Electronic address: hongzhou@ujs.edu.cn.
FAU - Zhang, Xiaolei
AU  - Zhang X
AD  - Department of Clinical Laboratory and Hematology, School of Medical Science and 
      Laboratory Medicine of Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
FAU - Liu, Jingjing
AU  - Liu J
AD  - Department of Clinical Laboratory and Hematology, School of Medical Science and 
      Laboratory Medicine of Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
FAU - Yan, Jinchuan
AU  - Yan J
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, School of 
      Medical Science and Laboratory Medicine of Jiangsu University, Zhenjiang, Jiangsu 
      212013, PR China. Electronic address: yanjinchuan@hotmail.com.
FAU - Xie, Hongxiang
AU  - Xie H
AD  - Department of Clinical Laboratory and Hematology, School of Medical Science and 
      Laboratory Medicine of Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
FAU - Xie, Yachao
AU  - Xie Y
AD  - Department of Clinical Laboratory and Hematology, School of Medical Science and 
      Laboratory Medicine of Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140520
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (NF-kappa B)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (beta 2-Glycoprotein I)
RN  - 0 (oxidized low density lipoprotein)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chemokine CCL2/immunology
MH  - Foam Cells/cytology/*immunology
MH  - Lipoproteins, LDL/*immunology
MH  - Macrophages, Peritoneal/cytology/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - NF-kappa B/*immunology
MH  - Signal Transduction
MH  - Toll-Like Receptor 4/*immunology
MH  - beta 2-Glycoprotein I/*immunology
OTO - NOTNLM
OT  - MCP-1
OT  - NF-kappaB
OT  - TF
OT  - TLR4
OT  - foam cell
OT  - oxLDL/beta2GPI/anti-beta2GPI complex
EDAT- 2014/06/03 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/06/03 06:00
PHST- 2013/12/30 00:00 [received]
PHST- 2014/04/11 00:00 [revised]
PHST- 2014/05/08 00:00 [accepted]
PHST- 2014/06/03 06:00 [entrez]
PHST- 2014/06/03 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - S0049-3848(14)00282-5 [pii]
AID - 10.1016/j.thromres.2014.05.017 [doi]
PST - ppublish
SO  - Thromb Res. 2014 Aug;134(2):384-92. doi: 10.1016/j.thromres.2014.05.017. Epub 
      2014 May 20.