PMID- 24882496 OWN - NLM STAT- MEDLINE DCOM- 20150527 LR - 20181202 IS - 1879-0984 (Electronic) IS - 0166-0934 (Linking) VI - 206 DP - 2014 Sep TI - Enhanced immune responses in pigs by DNA vaccine coexpressing GP3 and GP5 of European type porcine reproductive and respiratory syndrome virus. PG - 27-37 LID - S0166-0934(14)00213-4 [pii] LID - 10.1016/j.jviromet.2014.05.021 [doi] AB - The European (EU) type of porcine reproductive and respiratory syndrome virus (PRRSV) has recently emerged in China. In this study, three recombinant DNA vaccines, pVAX1-EU-ORF3-ORF5 (coexpressing EU type PRRSV GP3 and GP5), pVAX1-EU-ORF3 and pVAX1-EU-ORF5, were constructed and evaluated for their abilities to induce humoral and cellular responses as well as to protect piglets against homologous virus challenge. All piglets were given booster vaccinations at 21 days after the initial inoculation and then challenged 14 days later. Pigs inoculated with pVAX1-EU-ORF3-ORF5 developed significantly higher (P<0.05) PRRSV-specific antibody responses, neutralizing antibodies and levels of IL-4 and IL-10 than those given pVAX1-EU-ORF3, pVAX1-EU-ORF5 or pVAX1. Moreover, pigs immunized with pVAX1-EU-ORF3-ORF5 had markedly increased levels of IFN-gamma and IL-2 in serum and T-lymphocytes (CD3(+)CD4(+) and CD3(+)CD8(+) T cells) in peripheral blood. Thus, EU-type PRRSV GP3 and GP5 proteins demonstrated good immunogenicity and reactogenicity and could induce cellular immunity in pigs. Following challenge with the Lelystad virus (LV) strain, piglets inoculated with pVAX1-EU-ORF3-ORF5 showed viremia and virus load distributed in organ tissues that were significantly lower (P<0.05) than those in the pVAX1-EU-ORF3 group and control group, and slightly lower than those in the pVAX1-EU-ORF5 group (P>0.05). As GP3 could enhance humoral- and cell-mediated immune responses to GP5, the results of this study suggested that these two proteins delivered by a vaccine can synergistically induce immunity against PRRSV. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Ren, Jingqiang AU - Ren J AD - College of Veterinary Medicine, Jilin University, Changchun 130062, China; Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China. Electronic address: rjq207@163.com. FAU - Lu, Huijun AU - Lu H AD - Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China. Electronic address: nakata0007@sina.com. FAU - Wen, Shubo AU - Wen S AD - Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. Electronic address: wen0516@126.com. FAU - Sun, Wenchao AU - Sun W AD - Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. Electronic address: sunwenchao131@163.com. FAU - Yan, Fulong AU - Yan F AD - Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. Electronic address: yanfulong.xbmu@163.com. FAU - Chen, Xing AU - Chen X AD - Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. Electronic address: chenxing6067@126.com. FAU - Jing, Jie AU - Jing J AD - College of Veterinary Medicine, Jilin University, Changchun 130062, China; Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China. Electronic address: jingjie_0915@163.com. FAU - Liu, Hao AU - Liu H AD - Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China; Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130122, China. Electronic address: liuhaowinner@126.com. FAU - Liu, Cunxia AU - Liu C AD - Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China. Electronic address: liucunxia9@163.com. FAU - Xue, Fei AU - Xue F AD - Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China. Electronic address: 2224767289@qq.com. FAU - Xiao, Pengpeng AU - Xiao P AD - College of Veterinary Medicine, Jilin University, Changchun 130062, China; Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China. Electronic address: nick1103771759@gmail.com. FAU - Xin, Shu AU - Xin S AD - Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. Electronic address: 253691662@qq.com. FAU - Jin, Ningyi AU - Jin N AD - College of Veterinary Medicine, Jilin University, Changchun 130062, China; Institute of Military Veterinary, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, China. Electronic address: jinningyi2000@aliyun.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140529 PL - Netherlands TA - J Virol Methods JT - Journal of virological methods JID - 8005839 RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (Cytokines) RN - 0 (Glycoproteins) RN - 0 (Vaccines, DNA) RN - 0 (Vaccines, Synthetic) RN - 0 (Viral Proteins) RN - 0 (Viral Vaccines) SB - IM MH - Animal Structures/virology MH - Animals MH - Antibodies, Neutralizing/blood MH - Antibodies, Viral/blood MH - China MH - Cytokines/blood/metabolism MH - Glycoproteins/genetics/*immunology MH - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & control MH - Porcine respiratory and reproductive syndrome virus/genetics/*immunology MH - Swine MH - T-Lymphocytes/immunology MH - Vaccination/*methods MH - Vaccines, DNA/administration & dosage/genetics/*immunology MH - Vaccines, Synthetic/administration & dosage/genetics/immunology MH - Viral Load MH - Viral Proteins/genetics/*immunology MH - Viral Vaccines/administration & dosage/genetics/*immunology OTO - NOTNLM OT - DNA vaccine OT - European PRRSV OT - GP3 OT - GP5 OT - Immune responses OT - Pigs EDAT- 2014/06/03 06:00 MHDA- 2015/05/28 06:00 CRDT- 2014/06/03 06:00 PHST- 2014/01/12 00:00 [received] PHST- 2014/04/20 00:00 [revised] PHST- 2014/05/06 00:00 [accepted] PHST- 2014/06/03 06:00 [entrez] PHST- 2014/06/03 06:00 [pubmed] PHST- 2015/05/28 06:00 [medline] AID - S0166-0934(14)00213-4 [pii] AID - 10.1016/j.jviromet.2014.05.021 [doi] PST - ppublish SO - J Virol Methods. 2014 Sep;206:27-37. doi: 10.1016/j.jviromet.2014.05.021. Epub 2014 May 29.