PMID- 24884700
OWN - NLM
STAT- MEDLINE
DCOM- 20150124
LR  - 20240210
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 11
DP  - 2014 May 20
TI  - Mesoniviruses are mosquito-specific viruses with extensive geographic 
      distribution and host range.
PG  - 97
LID - 10.1186/1743-422X-11-97 [doi]
AB  - BACKGROUND: The family Mesoniviridae (order Nidovirales) comprises of a group of 
      positive-sense, single-stranded RNA ([+]ssRNA) viruses isolated from mosquitoes. 
      FINDINGS: Thirteen novel insect-specific virus isolates were obtained from 
      mosquitoes collected in Indonesia, Thailand and the USA. By electron microscopy, 
      the virions appeared as spherical particles with a diameter of ~50 nm. Their 
      20,129 nt to 20,777 nt genomes consist of positive-sense, single-stranded RNA 
      with a poly-A tail. Four isolates from Houston, Texas, and one isolate from Java, 
      Indonesia, were identified as variants of the species Alphamesonivirus-1 which 
      also includes Nam Dinh virus (NDiV) from Vietnam and Cavally virus (CavV) from 
      Cote d'Ivoire. The eight other isolates were identified as variants of three new 
      mesoniviruses, based on genome organization and pairwise evolutionary distances: 
      Karang Sari virus (KSaV) from Java, Bontag Baru virus (BBaV) from Java and 
      Kalimantan, and Kamphaeng Phet virus (KPhV) from Thailand. In comparison with 
      NDiV, the three new mesoniviruses each contained a long insertion (180 - 588 nt) 
      of unknown function in the 5' region of ORF1a, which accounted for much of the 
      difference in genome size. The insertions contained various short imperfect 
      repeats and may have arisen by recombination or sequence duplication. 
      CONCLUSIONS: In summary, based on their genome organizations and phylogenetic 
      relationships, thirteen new viruses were identified as members of the family 
      Mesoniviridae, order Nidovirales. Species demarcation criteria employed 
      previously for mesoniviruses would place five of these isolates in the same 
      species as NDiV and CavV (Alphamesonivirus-1) and the other eight isolates would 
      represent three new mesonivirus species (Alphamesonivirus-5, Alphamesonivirus-6 
      and Alphamesonivirus-7). The observed spatiotemporal distribution over widespread 
      geographic regions and broad species host range in mosquitoes suggests that 
      mesoniviruses may be common in mosquito populations worldwide.
FAU - Vasilakis, Nikos
AU  - Vasilakis N
AD  - Department of Pathology and Center for Biodefense and Emerging Infectious 
      Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, USA. 
      nivasila@utmb.edu.
FAU - Guzman, Hilda
AU  - Guzman H
FAU - Firth, Cadhla
AU  - Firth C
FAU - Forrester, Naomi L
AU  - Forrester NL
FAU - Widen, Steven G
AU  - Widen SG
FAU - Wood, Thomas G
AU  - Wood TG
FAU - Rossi, Shannan L
AU  - Rossi SL
FAU - Ghedin, Elodie
AU  - Ghedin E
FAU - Popov, Vsevolov
AU  - Popov V
FAU - Blasdell, Kim R
AU  - Blasdell KR
FAU - Walker, Peter J
AU  - Walker PJ
FAU - Tesh, Robert B
AU  - Tesh RB
LA  - eng
SI  - GENBANK/KC807166
SI  - GENBANK/KC807167
SI  - GENBANK/KC807168
SI  - GENBANK/KC807169
SI  - GENBANK/KC807170
SI  - GENBANK/KC807171
SI  - GENBANK/KC807172
SI  - GENBANK/KC807173
SI  - GENBANK/KC807174
SI  - GENBANK/KC807175
SI  - GENBANK/KC807176
SI  - GENBANK/KC807177
SI  - GENBANK/KC807178
GR  - HHSN272201000040I/HHSN27200004/D04/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140520
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Culicidae/*virology
MH  - Gene Order
MH  - *Host Specificity
MH  - Indonesia
MH  - Microscopy, Electron, Transmission
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Nidovirales/genetics/*isolation & purification/*physiology/ultrastructure
MH  - Nucleic Acid Conformation
MH  - *Phylogeography
MH  - RNA, Viral/genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Spatio-Temporal Analysis
MH  - Thailand
MH  - United States
MH  - Virion/ultrastructure
PMC - PMC4038087
EDAT- 2014/06/03 06:00
MHDA- 2015/01/27 06:00
PMCR- 2014/05/20
CRDT- 2014/06/03 06:00
PHST- 2014/02/03 00:00 [received]
PHST- 2014/05/05 00:00 [accepted]
PHST- 2014/06/03 06:00 [entrez]
PHST- 2014/06/03 06:00 [pubmed]
PHST- 2015/01/27 06:00 [medline]
PHST- 2014/05/20 00:00 [pmc-release]
AID - 1743-422X-11-97 [pii]
AID - 10.1186/1743-422X-11-97 [doi]
PST - epublish
SO  - Virol J. 2014 May 20;11:97. doi: 10.1186/1743-422X-11-97.