PMID- 24886296
OWN - NLM
STAT- MEDLINE
DCOM- 20150223
LR  - 20211021
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 12
DP  - 2014 May 11
TI  - Repair of orbital bone defects in canines using grafts of enriched autologous 
      bone marrow stromal cells.
PG  - 123
LID - 10.1186/1479-5876-12-123 [doi]
AB  - BACKGROUND: Bone tissue engineering is a new approach for the repair of orbital 
      defects. The aim of the present study was to explore the feasibility of 
      tissue-engineered bone constructed using bone marrow stromal cells (BMSCs) that 
      were rapidly isolated and concentrated from bone marrow (BM) by the red cell 
      lysis method, then combined with beta-tricalcium phosphate (beta-TCP) to create grafts 
      used to restore orbital bone defects in canines. METHODS: In the experimental 
      group, grafts were constructed using BMSCs obtained by red cell lysis from 20 ml 
      bone marrow, combined with beta-TCP and BM via the custom-made stem cell-scaffold 
      device, then used to repair 10 mm diameter medial orbital wall bony defects in 
      canines. Results were compared with those in groups grafted with BM/beta-TCP or 
      beta-TCP alone, or with defects left untreated as controls. The enrichment of BMSCs 
      and nucleated cells (NCs) in the graft was calculated from the number in 
      untreated bone marrow and in suspensions after red cell lysis. Spiral computed 
      tomography (CT) scans were performed 1, 4, 12 and 24 weeks after implantation in 
      all groups. Gross examination, micro-CT and histological measurements were 
      performed 24 weeks after surgery. The results were analyzed to evaluate the 
      efficacy of bone repair. RESULTS: The number of NCs and of colony-forming units 
      within the scaffolds were increased 54.8 times and 53.4 times, respectively, 
      compared with untreated bone marrow. In the BMSC-BM/beta-TCP group, CT examination 
      revealed that the scaffolds were gradually absorbed and the bony defects were 
      restored. Micro-CT and histological examination confirmed that the implantations 
      led to good repair of the defects, with 6 out 8 orbital defects completely 
      restored in the experimental group, while by contrast, the grafts in the control 
      groups did not fully repair the bony defects, a difference which was 
      statistically significant (p<0.05). CONCLUSIONS: Tissue-engineered bone, 
      constructed using BMSCs isolated by red cell lysis of BM, can restore 
      critical-sized orbital wall defects in canines.
FAU - Wang, Yefei
AU  - Wang Y
FAU - Bi, Xiaoping
AU  - Bi X
FAU - Zhou, Huifang
AU  - Zhou H
FAU - Deng, Yuan
AU  - Deng Y
FAU - Sun, Jing
AU  - Sun J
FAU - Xiao, Caiwen
AU  - Xiao C
FAU - Gu, Ping
AU  - Gu P
FAU - Fan, Xianqun
AU  - Fan X
AD  - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China. fanxq@sh163.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140511
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
SB  - IM
MH  - Animals
MH  - Cuspid/*abnormalities/diagnostic imaging
MH  - Dogs
MH  - Mesenchymal Stem Cells/*cytology
MH  - Orbit/*abnormalities/diagnostic imaging
MH  - *Stem Cell Transplantation
MH  - Tomography, Spiral Computed
MH  - X-Ray Microtomography
PMC - PMC4036112
EDAT- 2014/06/03 06:00
MHDA- 2015/02/24 06:00
PMCR- 2014/05/11
CRDT- 2014/06/03 06:00
PHST- 2014/02/13 00:00 [received]
PHST- 2014/04/28 00:00 [accepted]
PHST- 2014/06/03 06:00 [entrez]
PHST- 2014/06/03 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PHST- 2014/05/11 00:00 [pmc-release]
AID - 1479-5876-12-123 [pii]
AID - 10.1186/1479-5876-12-123 [doi]
PST - epublish
SO  - J Transl Med. 2014 May 11;12:123. doi: 10.1186/1479-5876-12-123.