PMID- 24887492
OWN - NLM
STAT- MEDLINE
DCOM- 20150120
LR  - 20220409
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 6
DP  - 2014
TI  - Human umbilical cord-derived mesenchymal stem cells do not undergo malignant 
      transformation during long-term culturing in serum-free medium.
PG  - e98565
LID - 10.1371/journal.pone.0098565 [doi]
LID - e98565
AB  - BACKGROUND: Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are in 
      the foreground as a preferable application for treating diseases. However, the 
      safety of hUC-MSCs after long-term culturing in vitro in serum-free medium 
      remains unclear. METHODS: hUC-MSCs were separated by adherent tissue culture. 
      hUC-MSCs were cultured in serum-free MesenCult-XF medium and FBS-bases DMEM 
      complete medium. At the 1st, 3rd, 5th, 8th, 10th, and 15th passage, the 
      differentiation of MSCs into osteogenic, chondrogenic, and adipogenic cells was 
      detected, and MTT, surface antigens were measured. Tumorigenicity was analyzed at 
      the 15th passage. Conventional karyotyping was performed at passage 0, 8, and 15. 
      The telomerase activity of hUC-MSCs at passage 1-15 was analyzed. RESULTS: Flow 
      cytometry analysis showed that very high expression was detected for CD105, CD73, 
      and CD90 and very low expression for CD45, CD34, CD14, CD79a, and HLA-DR. MSCs 
      could differentiate into osteocytes, chondrocytes, and adipocytes in vitro. There 
      was no obvious chromosome elimination, displacement, or chromosomal imbalance as 
      determined from the guidelines of the International System for Human Cytogenetic 
      Nomenclature. Telomerase activity was down-regulated significantly when the 
      culture time was prolonged. Further, no tumors formed in rats injected with 
      hUC-MSCs (P15) cultured in serum-free and in serum-containing conditions. 
      CONCLUSION: Our data showed that hUC-MSCs met the International Society for 
      Cellular Therapy standards for conditions of long-term in vitro culturing at P15. 
      Since hUC-MSCs can be safely expanded in vitro and are not susceptible to 
      malignant transformation in serum-free medium, these cells are suitable for cell 
      therapy.
FAU - Chen, Gecai
AU  - Chen G
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu province, 
      China.
FAU - Yue, Aihuan
AU  - Yue A
AD  - Jiangsu Beike Bio-Technology Co., Ltd, Taizhou, Jiangsu province, China.
FAU - Ruan, Zhongbao
AU  - Ruan Z
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu province, 
      China.
FAU - Yin, Yigang
AU  - Yin Y
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu province, 
      China.
FAU - Wang, RuZhu
AU  - Wang R
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu province, 
      China.
FAU - Ren, Yin
AU  - Ren Y
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu province, 
      China.
FAU - Zhu, Li
AU  - Zhu L
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu province, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20140602
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Culture Media, Serum-Free)
SB  - IM
MH  - *Cell Transformation, Neoplastic
MH  - Cells, Cultured
MH  - Culture Media, Serum-Free
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - Karyotyping
MH  - Mesenchymal Stem Cells/*cytology/immunology
MH  - Umbilical Cord/*cytology
PMC - PMC4041760
COIS- Competing Interests: The authors have read the journal's policy and have the 
      following conflicts: Co-author Aihuan Yue is employed by Jiangsu Beike 
      Bio-Technology Co., Ltd. This does not alter the authors' adherence to PLOS ONE 
      policies on sharing data and materials.
EDAT- 2014/06/03 06:00
MHDA- 2015/01/21 06:00
PMCR- 2014/06/02
CRDT- 2014/06/03 06:00
PHST- 2014/02/12 00:00 [received]
PHST- 2014/05/05 00:00 [accepted]
PHST- 2014/06/03 06:00 [entrez]
PHST- 2014/06/03 06:00 [pubmed]
PHST- 2015/01/21 06:00 [medline]
PHST- 2014/06/02 00:00 [pmc-release]
AID - PONE-D-14-02137 [pii]
AID - 10.1371/journal.pone.0098565 [doi]
PST - epublish
SO  - PLoS One. 2014 Jun 2;9(6):e98565. doi: 10.1371/journal.pone.0098565. eCollection 
      2014.