PMID- 24889884
OWN - NLM
STAT- MEDLINE
DCOM- 20171214
LR  - 20220310
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 11
IP  - 2
DP  - 2017 Feb
TI  - Extracorporeal shockwaves (ESWs) enhance the osteogenic medium-induced 
      differentiation of adipose-derived stem cells into osteoblast-like cells.
PG  - 390-399
LID - 10.1002/term.1922 [doi]
AB  - Human adipose-derived stem cells (hASCs) are a promising cell type for bone 
      tissue engineering, given their potential to differentiate into osteoblast-like 
      cells. Interactions among biochemical and mechanical signals result in bone 
      formation and repair. In this process stem cells have a crucial role. 
      Extracorporeal shockwaves (ESWs) are acoustic waves capable of enhancing bone 
      regeneration, suggesting that ESWs may induce some signals for mesenchymal 
      progenitor maturation. The aim of the present work is to investigate the effects 
      of ESW treatment on the differentiation of hASCs into osteoblast-like cells and 
      to better clarify the mechanisms involved. The hASCs were treated with ESWs and 
      osteogenic medium, and the effects in terms of gene expression, alkaline 
      phosphatase (ALP) activity and calcium deposition were then evaluated. Moreover, 
      to investigate the mechanisms of ESW action, reactive oxygen species (ROS) 
      production, extracellular-signal-regulated kinase (ERK) and small 'mothers 
      against' decapentaplegic (Smad) phosphorylation, and bone morphogenetic protein 2 
      (BMP2) expression were assessed. The ESW treatment increased Runt-related 
      transcription factor 2 (Runx2), ALP and BMP2 expression, as well as ALP activity 
      and calcium deposits with respect to untreated cells. Moreover ESWs induced ROS 
      formation, and both ERK and Smad phosphorylation. The present study shows the 
      effects of ESWs on osteogenic differentiation in an in vitro model using hASCs 
      and defines the mechanisms involved in this process. The observations suggest 
      that the combination of autologous hASCs and ESW treatment may improve bone 
      tissue repair in tissue engineering procedures. Copyright (c) 2014 John Wiley & 
      Sons, Ltd.
CI  - Copyright (c) 2014 John Wiley & Sons, Ltd.
FAU - Catalano, Maria Graziella
AU  - Catalano MG
AD  - Department of Medical Sciences, University of Turin, Turin, Italy.
FAU - Marano, Francesca
AU  - Marano F
AD  - Department of Medical Sciences, University of Turin, Turin, Italy.
FAU - Rinella, Letizia
AU  - Rinella L
AD  - Department of Medical Sciences, University of Turin, Turin, Italy.
FAU - de Girolamo, Laura
AU  - de Girolamo L
AD  - Orthopaedic Biotechnologies Laboratory, IRCCS Istituto Ortopedico Galeazzi, 
      Milan, Italy.
FAU - Bosco, Ornella
AU  - Bosco O
AD  - Department of Medical Sciences, University of Turin, Turin, Italy.
FAU - Fortunati, Nicoletta
AU  - Fortunati N
AD  - Oncological Endocrinology, AO Citta della Salute e della Scienza di Torino, 
      Turin, Italy.
FAU - Berta, Laura
AU  - Berta L
AD  - Med&Sport 2000 Srl, Turin, Italy.
FAU - Frairia, Roberto
AU  - Frairia R
AD  - Department of Medical Sciences, University of Turin, Turin, Italy.
LA  - eng
PT  - Journal Article
DEP - 20140601
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Culture Media)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Alkaline Phosphatase/metabolism
MH  - Bone Morphogenetic Protein 2/metabolism
MH  - *Bone Regeneration
MH  - Bone and Bones/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Culture Media/chemistry
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - *High-Energy Shock Waves
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - Osteoblasts/*cytology/drug effects
MH  - Osteogenesis/*drug effects
MH  - Phosphorylation
MH  - Reactive Oxygen Species/metabolism
MH  - Tissue Engineering/methods
OTO - NOTNLM
OT  - adipose-derived stem cells (ASCs)
OT  - bone
OT  - extracorporeal shockwaves (ESWs)
OT  - osteogenic differentiation
EDAT- 2014/06/04 06:00
MHDA- 2017/12/15 06:00
CRDT- 2014/06/04 06:00
PHST- 2013/06/07 00:00 [received]
PHST- 2014/03/07 00:00 [revised]
PHST- 2014/04/24 00:00 [accepted]
PHST- 2014/06/04 06:00 [pubmed]
PHST- 2017/12/15 06:00 [medline]
PHST- 2014/06/04 06:00 [entrez]
AID - 10.1002/term.1922 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2017 Feb;11(2):390-399. doi: 10.1002/term.1922. Epub 2014 
      Jun 1.