PMID- 24890472
OWN - NLM
STAT- MEDLINE
DCOM- 20150522
LR  - 20140925
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 111
IP  - 11
DP  - 2014 Nov
TI  - Bacterial community structure and activity of sulfate reducing bacteria in a 
      membrane aerated biofilm analyzed by microsensor and molecular techniques.
PG  - 2155-62
LID - 10.1002/bit.25277 [doi]
AB  - The activities and vertical spatial distribution of sulfate reducing bacteria 
      (SRB) in an oxygen (O2 )-based membrane aerated biofilm (MAB) were investigated 
      using microsensor (O2 and H2 S) measurements and molecular techniques (polymerase 
      chain reaction-denaturing gradient gel electrophoresis [PCR-DGGE] and 
      fluorescence in situ hybridization [FISH]). The O2 concentration profile revealed 
      that O2 penetrated from the bottom (substratum) of the gas permeable membrane, 
      and was gradually consumed within the biofilm until it was completely depleted 
      near the biofilm/bulk liquid interface, indicating oxic and anoxic zone in the 
      MAB. The H2 S concentration profile showed that H2 S production was found in the 
      upper 285 microm of the biofilm, indicating a high activity of SRB in this region. 
      The results from DGGE of the PCR-amplified dissimilatory sulfite reductase 
      subunit B (dsrB) gene and FISH showed an uneven spatial distribution of SRB. The 
      maximum SRB biomass was located in the upper biofilm. The information from the 
      molecular analysis can be supplemented with that from microsensor measurements to 
      better understand the microbial community and activity of SRB in the MAB.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Liu, Hong
AU  - Liu H
AD  - Department of Civil and Environmental Engineering, University of Alberta, 
      Edmonton, Alberta, Canada, T6G 2W2.
FAU - Tan, Shuying
AU  - Tan S
FAU - Sheng, Zhiya
AU  - Sheng Z
FAU - Liu, Yang
AU  - Liu Y
FAU - Yu, Tong
AU  - Yu T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140704
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Sulfates)
RN  - S88TT14065 (Oxygen)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Aerobiosis
MH  - Bacteria, Aerobic/*classification/*isolation & purification
MH  - Biofilms/*growth & development
MH  - *Biota
MH  - Denaturing Gradient Gel Electrophoresis
MH  - *Environmental Microbiology
MH  - Hydrogen Sulfide/analysis
MH  - In Situ Hybridization, Fluorescence
MH  - Membranes
MH  - Oxidation-Reduction
MH  - Oxygen/analysis
MH  - Polymerase Chain Reaction
MH  - Sulfates/*metabolism
OTO - NOTNLM
OT  - membrane aerated biofilm
OT  - microsensor
OT  - molecular technique
OT  - sulfate reducing bacteria
EDAT- 2014/06/04 06:00
MHDA- 2015/05/23 06:00
CRDT- 2014/06/04 06:00
PHST- 2014/01/13 00:00 [received]
PHST- 2014/04/23 00:00 [revised]
PHST- 2014/04/29 00:00 [accepted]
PHST- 2014/06/04 06:00 [entrez]
PHST- 2014/06/04 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
AID - 10.1002/bit.25277 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2014 Nov;111(11):2155-62. doi: 10.1002/bit.25277. Epub 2014 
      Jul 4.