PMID- 24891509
OWN - NLM
STAT- MEDLINE
DCOM- 20141006
LR  - 20220129
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 289
IP  - 29
DP  - 2014 Jul 18
TI  - The nuclear export protein of H5N1 influenza A viruses recruits Matrix 1 (M1) 
      protein to the viral ribonucleoprotein to mediate nuclear export.
PG  - 20067-77
LID - 10.1074/jbc.M114.569178 [doi]
AB  - In influenza A virus-infected cells, replication and transcription of the viral 
      genome occurs in the nucleus. To be packaged into viral particles at the plasma 
      membrane, encapsidated viral genomes must be exported from the nucleus. 
      Intriguingly, the nuclear export protein (NEP) is involved in both processes. 
      Although NEP stimulates viral RNA synthesis by binding to the viral polymerase, 
      its function during nuclear export implicates interaction with viral 
      ribonucleoprotein (vRNP)-associated M1. The observation that both interactions 
      are mediated by the C-terminal moiety of NEP raised the question whether these 
      two features of NEP are linked functionally. Here we provide evidence that the 
      interaction between M1 and the vRNP depends on the NEP C terminus and its 
      polymerase activity-enhancing property for the nuclear export of vRNPs. This 
      suggests that these features of NEP are linked functionally. Furthermore, our 
      data suggest that the N-terminal domain of NEP interferes with the stability of 
      the vRNP-M1-NEP nuclear export complex, probably mediated by its highly flexible 
      intramolecular interaction with the NEP C terminus. On the basis of our data, we 
      propose a new model for the assembly of the nuclear export complex of Influenza A 
      vRNPs.
CI  - (c) 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
FAU - Brunotte, Linda
AU  - Brunotte L
AD  - From the Institute for Virology, University Medical Center Freiburg, 
      Hermann-Herder-Str. 11, 79104 Freiburg, Germany and.
FAU - Flies, Joe
AU  - Flies J
AD  - From the Institute for Virology, University Medical Center Freiburg, 
      Hermann-Herder-Str. 11, 79104 Freiburg, Germany and.
FAU - Bolte, Hardin
AU  - Bolte H
AD  - From the Institute for Virology, University Medical Center Freiburg, 
      Hermann-Herder-Str. 11, 79104 Freiburg, Germany and.
FAU - Reuther, Peter
AU  - Reuther P
AD  - From the Institute for Virology, University Medical Center Freiburg, 
      Hermann-Herder-Str. 11, 79104 Freiburg, Germany and.
FAU - Vreede, Frank
AU  - Vreede F
AD  - the Sir William Dunn School of Pathology, University of Oxford, South Parks Rd., 
      Oxford OX 3RE, United Kingdom.
FAU - Schwemmle, Martin
AU  - Schwemmle M
AD  - From the Institute for Virology, University Medical Center Freiburg, 
      Hermann-Herder-Str. 11, 79104 Freiburg, Germany and 
      martin.schwemmle@uniklinik-freiburg.de.
LA  - eng
GR  - G1100138/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140602
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (M1 protein, Influenza A virus)
RN  - 0 (Nucleocytoplasmic Transport Proteins)
RN  - 0 (RNA, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - HEK293 Cells
MH  - Humans
MH  - Influenza A Virus, H5N1 Subtype/genetics/*metabolism
MH  - Models, Biological
MH  - Nucleocytoplasmic Transport Proteins/chemistry/genetics/*metabolism
MH  - Protein Interaction Domains and Motifs
MH  - RNA, Viral/biosynthesis/genetics
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Ribonucleoproteins/chemistry/genetics/*metabolism
MH  - Sequence Deletion
MH  - Transcription, Genetic
MH  - Viral Matrix Proteins/chemistry/genetics/*metabolism
MH  - Viral Proteins/chemistry/genetics/*metabolism
MH  - Virus Replication
PMC - PMC4106323
OTO - NOTNLM
OT  - Influenza Virus
OT  - Protein Export
OT  - Ribonucleoprotein (RNP)
OT  - Viral Polymerase
OT  - Viral Protein
EDAT- 2014/06/04 06:00
MHDA- 2014/10/07 06:00
PMCR- 2015/07/18
CRDT- 2014/06/04 06:00
PHST- 2014/06/04 06:00 [entrez]
PHST- 2014/06/04 06:00 [pubmed]
PHST- 2014/10/07 06:00 [medline]
PHST- 2015/07/18 00:00 [pmc-release]
AID - S0021-9258(20)47656-2 [pii]
AID - M114.569178 [pii]
AID - 10.1074/jbc.M114.569178 [doi]
PST - ppublish
SO  - J Biol Chem. 2014 Jul 18;289(29):20067-77. doi: 10.1074/jbc.M114.569178. Epub 
      2014 Jun 2.