PMID- 24893147
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20140722
IS  - 1096-0384 (Electronic)
IS  - 0003-9861 (Linking)
VI  - 557
DP  - 2014 Sep 1
TI  - Effects of the melanin precursor 5,6-dihydroxy-indole-2-carboxylic acid (DHICA) 
      on DNA damage and repair in the presence of reactive oxygen species.
PG  - 55-64
LID - S0003-9861(14)00189-1 [pii]
LID - 10.1016/j.abb.2014.05.024 [doi]
AB  - Eumelanin is a heterogeneous polymer composed of 5,6-dihydroxyindole-2-carboxylic 
      acid (DHICA) and 5,6-dihydroxyindole (DHI). Studies have shown that DHICA 
      promotes single strand breaks in plasmid DNA exposed to ultraviolet B radiation 
      (UVB, 313 nm) and in DNA from human keratinocytes exposed to ultraviolet A 
      radiation (UVA, 340-400 nm). Singlet molecular oxygen ((1)O2) is the main 
      reactive species formed by UVA radiation on the skin. In this context, we now 
      report that DHICA can cause single strand breaks in plasmid DNA even in the 
      absence of light radiation. Interestingly, when DHICA was pre-oxidized by (1)O2, 
      it lost this harmful capacity. It was also found that DHICA could interact with 
      DNA, disturbing Fpg activity and decreasing its recognition of lesions by  approximately 50%. 
      Additionally, the free nucleoside deoxyguanosine (dGuo) was used to evaluate 
      whether DHICA would interfere with the formation of 
      8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and spiroiminodihydantoin (dSp) 
      by (1)O2 or with the formation of 8-oxodGuo by hydroxyl radical (OH). We observed 
      that when dGuo was oxidized by (1)O2 in the presence of DHICA, 8-oxodGuo 
      formation was increased. However, when dGuo was oxidized by OH in the presence of 
      DHICA, 8-oxodGuo levels were lower than in the absence of the precursor. Overall, 
      our data reveal an important role for this eumelanin precursor in both the 
      promotion and the protection of DNA damage and imply that it can impair DNA 
      repair.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Pellosi, Maria Carolina
AU  - Pellosi MC
AD  - Programa de Pos-graduacao em Ciencias (Bioquimica), Departamento de Bioquimica e 
      Biologia Molecular, Universidade Federal do Parana, Curitiba, PR, Brazil.
FAU - Suzukawa, Andreia Akemi
AU  - Suzukawa AA
AD  - Programa de Pos-graduacao em Ciencias (Bioquimica), Departamento de Bioquimica e 
      Biologia Molecular, Universidade Federal do Parana, Curitiba, PR, Brazil.
FAU - Scalfo, Alexsandra Cristina
AU  - Scalfo AC
AD  - Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao 
      Paulo, SP, Brazil.
FAU - Di Mascio, Paolo
AU  - Di Mascio P
AD  - Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao 
      Paulo, SP, Brazil.
FAU - Martins Pereira, Carolina Parga
AU  - Martins Pereira CP
AD  - Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao 
      Paulo, SP, Brazil.
FAU - de Souza Pinto, Nadja Cristhina
AU  - de Souza Pinto NC
AD  - Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao 
      Paulo, SP, Brazil.
FAU - de Luna Martins, Daniela
AU  - de Luna Martins D
AD  - Instituto de Quimica, Universidade Federal Fluminense, Rio de Janeiro, RJ, 
      Brazil.
FAU - Martinez, Glaucia Regina
AU  - Martinez GR
AD  - Programa de Pos-graduacao em Ciencias (Bioquimica), Departamento de Bioquimica e 
      Biologia Molecular, Universidade Federal do Parana, Curitiba, PR, Brazil. 
      Electronic address: grmartinez@pq.cnpq.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140602
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Indoles)
RN  - 0 (Reactive Oxygen Species)
RN  - 4790-08-3 (5,6-dihydroxy-2-indolylcarboxylic acid)
SB  - IM
MH  - *DNA Damage
MH  - *DNA Repair
MH  - Indoles/*chemistry
MH  - Reactive Oxygen Species/*chemistry
OTO - NOTNLM
OT  - DHICA
OT  - DNA damage
OT  - DNA repair
OT  - Fpg
OT  - Hydroxyl radical
OT  - Singlet oxygen
OT  - Strand breaks
EDAT- 2014/06/04 06:00
MHDA- 2014/09/16 06:00
CRDT- 2014/06/04 06:00
PHST- 2014/01/14 00:00 [received]
PHST- 2014/05/15 00:00 [revised]
PHST- 2014/05/26 00:00 [accepted]
PHST- 2014/06/04 06:00 [entrez]
PHST- 2014/06/04 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
AID - S0003-9861(14)00189-1 [pii]
AID - 10.1016/j.abb.2014.05.024 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2014 Sep 1;557:55-64. doi: 10.1016/j.abb.2014.05.024. Epub 
      2014 Jun 2.